Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEN9T_RS21885 Genome accession   NZ_CP168610
Coordinates   2764613..2765113 (-) Length   166 a.a.
NCBI ID   WP_413707048.1    Uniprot ID   -
Organism   Ralstonia sp. Ralssp110 strain CT11-110     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2759613..2770113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9T_RS21865 (ACEN9T_21860) - 2759787..2761490 (-) 1704 WP_413707045.1 M48 family metalloprotease -
  ACEN9T_RS21870 (ACEN9T_21865) moaC 2761596..2762072 (+) 477 WP_413707046.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACEN9T_RS21875 (ACEN9T_21870) pilA 2762132..2762578 (-) 447 WP_413708496.1 pilin Machinery gene
  ACEN9T_RS21880 (ACEN9T_21875) - 2762810..2764540 (-) 1731 WP_413707047.1 Wzy polymerase domain-containing protein -
  ACEN9T_RS21885 (ACEN9T_21880) pilA 2764613..2765113 (-) 501 WP_413707048.1 pilin Machinery gene
  ACEN9T_RS21890 (ACEN9T_21885) pilA 2765386..2765910 (-) 525 WP_413707049.1 pilin Machinery gene
  ACEN9T_RS21895 (ACEN9T_21890) - 2766096..2766794 (-) 699 WP_413708497.1 TerC family protein -
  ACEN9T_RS21900 (ACEN9T_21895) sucD 2766955..2767836 (-) 882 WP_009239052.1 succinate--CoA ligase subunit alpha -
  ACEN9T_RS21905 (ACEN9T_21900) sucC 2767925..2769091 (-) 1167 WP_092973140.1 ADP-forming succinate--CoA ligase subunit beta -
  ACEN9T_RS21910 (ACEN9T_21905) - 2769158..2769784 (-) 627 WP_169341807.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 166 a.a.        Molecular weight: 17117.80 Da        Isoelectric Point: 9.8719

>NTDB_id=1044616 ACEN9T_RS21885 WP_413707048.1 2764613..2765113(-) (pilA) [Ralstonia sp. Ralssp110 strain CT11-110]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKALVAENAANAQTDLSVGSSVFT
ATKNVSNMAIASGNGEIQLTYTQAAGNGTLVLKPAASGVALAAGTVPGTNIQWGCFAAGKAATSQVPAPTTYTYTLLAKY
APAECR

Nucleotide


Download         Length: 501 bp        

>NTDB_id=1044616 ACEN9T_RS21885 WP_413707048.1 2764613..2765113(-) (pilA) [Ralstonia sp. Ralssp110 strain CT11-110]
ATGAAATCGATGCGTCATCTGAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCCATCGT
CGGTATTCTGGCCGCCATCGCCATTCCGGCGTACCAGGATTACACGATCCGTGCACGCGTAACGGAAGGTCTGTCGCTGG
CTGCACAAGCTAAGGCATTGGTGGCTGAAAATGCTGCGAACGCTCAGACCGATTTGTCGGTCGGTTCCTCCGTTTTTACC
GCTACGAAAAACGTTTCTAACATGGCCATCGCTTCAGGCAACGGTGAGATTCAACTCACCTACACGCAGGCCGCTGGGAA
CGGCACCCTGGTCTTGAAGCCGGCAGCAAGCGGTGTTGCGCTGGCAGCGGGGACTGTCCCCGGTACCAACATTCAATGGG
GTTGCTTCGCTGCTGGTAAAGCTGCGACTTCGCAAGTGCCGGCTCCGACGACATACACGTACACGTTGCTGGCAAAGTAC
GCACCGGCAGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(44-166)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

71.598

100

0.729
  pilA2 Legionella pneumophila str. Paris

50.932

96.988

0.494
  pilA2 Legionella pneumophila strain ERS1305867

50.311

96.988

0.488
  comP Acinetobacter baylyi ADP1

42.105

100

0.434
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.392

100

0.392

Multiple sequence alignment