Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEN9T_RS21875 Genome accession   NZ_CP168610
Coordinates   2762132..2762578 (-) Length   148 a.a.
NCBI ID   WP_413708496.1    Uniprot ID   -
Organism   Ralstonia sp. Ralssp110 strain CT11-110     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2757132..2767578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9T_RS21850 (ACEN9T_21845) - 2757646..2758086 (+) 441 WP_024976550.1 YybH family protein -
  ACEN9T_RS21855 (ACEN9T_21850) - 2758121..2759197 (+) 1077 WP_413707043.1 YheT family hydrolase -
  ACEN9T_RS21860 (ACEN9T_21855) - 2759190..2759771 (+) 582 WP_413707044.1 DUF2946 family protein -
  ACEN9T_RS21865 (ACEN9T_21860) - 2759787..2761490 (-) 1704 WP_413707045.1 M48 family metalloprotease -
  ACEN9T_RS21870 (ACEN9T_21865) moaC 2761596..2762072 (+) 477 WP_413707046.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACEN9T_RS21875 (ACEN9T_21870) pilA 2762132..2762578 (-) 447 WP_413708496.1 pilin Machinery gene
  ACEN9T_RS21880 (ACEN9T_21875) - 2762810..2764540 (-) 1731 WP_413707047.1 Wzy polymerase domain-containing protein -
  ACEN9T_RS21885 (ACEN9T_21880) pilA 2764613..2765113 (-) 501 WP_413707048.1 pilin Machinery gene
  ACEN9T_RS21890 (ACEN9T_21885) pilA 2765386..2765910 (-) 525 WP_413707049.1 pilin Machinery gene
  ACEN9T_RS21895 (ACEN9T_21890) - 2766096..2766794 (-) 699 WP_413708497.1 TerC family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15483.60 Da        Isoelectric Point: 4.8282

>NTDB_id=1044615 ACEN9T_RS21875 WP_413708496.1 2762132..2762578(-) (pilA) [Ralstonia sp. Ralssp110 strain CT11-110]
MIVVAIIGVLASLAVPAYQDYTIRAKVTEGLTFAAQTKTRVAENAIAGQSDLSRGMSTFLATKNVSDLAVDKETGEIVVT
YDSRMVPTDKSKLVLTPYSGGTNLQAGSVPAAAIQWVCAASGKAPDDASVTQNTEPTLEAKYAPAECR

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1044615 ACEN9T_RS21875 WP_413708496.1 2762132..2762578(-) (pilA) [Ralstonia sp. Ralssp110 strain CT11-110]
ATGATAGTTGTTGCAATCATTGGGGTCCTAGCGTCCCTCGCCGTGCCGGCATATCAGGACTACACCATCCGTGCAAAGGT
TACAGAAGGGTTAACATTCGCCGCACAAACCAAGACTCGAGTTGCGGAGAATGCCATTGCCGGGCAGAGCGACTTATCCA
GGGGGATGTCTACATTCTTGGCAACCAAAAACGTTTCAGATTTAGCGGTCGACAAAGAGACCGGTGAAATTGTTGTGACC
TACGATTCCAGAATGGTGCCGACCGACAAGAGCAAGCTTGTTCTGACGCCGTACTCAGGCGGTACAAACTTGCAGGCTGG
GAGTGTTCCTGCCGCCGCAATTCAATGGGTCTGTGCTGCAAGTGGAAAGGCGCCTGACGACGCTAGTGTTACGCAAAATA
CTGAACCGACTCTGGAAGCCAAATACGCTCCAGCAGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(24-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50.331

100

0.514
  pilA2 Legionella pneumophila str. Paris

40.132

100

0.412
  pilA2 Legionella pneumophila strain ERS1305867

39.474

100

0.405

Multiple sequence alignment