Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEN9J_RS26315 Genome accession   NZ_CP168540
Coordinates   5678264..5679997 (-) Length   577 a.a.
NCBI ID   WP_057592120.1    Uniprot ID   -
Organism   Variovorax sp. Varisp41 strain CT11-41     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5673264..5684997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9J_RS26295 (ACEN9J_26295) - 5673843..5675333 (-) 1491 WP_057592102.1 MBOAT family O-acyltransferase -
  ACEN9J_RS26300 (ACEN9J_26300) coaE 5675408..5676016 (-) 609 WP_172705374.1 dephospho-CoA kinase -
  ACEN9J_RS26305 (ACEN9J_26305) - 5676033..5676998 (-) 966 WP_296729628.1 prepilin peptidase -
  ACEN9J_RS26310 (ACEN9J_26310) pilC 5676998..5678233 (-) 1236 WP_057592099.1 type II secretion system F family protein Machinery gene
  ACEN9J_RS26315 (ACEN9J_26315) pilB 5678264..5679997 (-) 1734 WP_057592120.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN9J_RS26325 (ACEN9J_26325) - 5680240..5681232 (-) 993 WP_057592098.1 polyprenyl synthetase family protein -
  ACEN9J_RS26330 (ACEN9J_26330) rplU 5681417..5681728 (+) 312 WP_057592097.1 50S ribosomal protein L21 -
  ACEN9J_RS26335 (ACEN9J_26335) rpmA 5681741..5682001 (+) 261 WP_057592096.1 50S ribosomal protein L27 -
  ACEN9J_RS26340 (ACEN9J_26340) cgtA 5682073..5683149 (+) 1077 WP_413762468.1 Obg family GTPase CgtA -
  ACEN9J_RS26345 (ACEN9J_26345) proB 5683228..5684367 (+) 1140 WP_413762469.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62618.83 Da        Isoelectric Point: 5.8821

>NTDB_id=1044528 ACEN9J_RS26315 WP_057592120.1 5678264..5679997(-) (pilB) [Variovorax sp. Varisp41 strain CT11-41]
MAAAELPVKENPQVALPGLARALVSAGKLPAKTAEDLYQKSLSSRSSFIAELTGTGAVSAADLAHTLSNAFGAPLLDLDA
IDHQRLPKDLLDPKLCQAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMVEAAAASAAETI
NSIVGTEFEFDDVTADTAGDANEQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREVASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDADEKER
LLHAISRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCMTCRTPADIPR
EALIEAGFQEHEVDGSWKPYRPVGCAACTGGYKGRVGIYQVMPISEAIQEIILRDGSALDIAQQSEAEGVRSLRKSGLRK
VMQGLTSLEEVVAVTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1044528 ACEN9J_RS26315 WP_057592120.1 5678264..5679997(-) (pilB) [Variovorax sp. Varisp41 strain CT11-41]
ATGGCTGCAGCCGAACTTCCCGTTAAAGAAAACCCGCAAGTCGCCTTGCCAGGGCTTGCACGCGCGCTGGTTTCGGCCGG
CAAGCTGCCCGCGAAGACCGCGGAAGATCTCTATCAGAAGTCGCTGAGCAGCCGCTCCAGTTTCATCGCCGAGCTCACGG
GCACCGGCGCCGTCTCGGCCGCCGACCTCGCCCACACGCTCTCGAATGCGTTCGGCGCCCCGCTGCTCGACCTCGACGCC
ATCGACCACCAGCGCCTGCCGAAGGACCTGCTCGATCCCAAGCTCTGCCAGGCCTACCGCATCGTCGTCCTCAGCAAGCG
CAACAACCGCCTGATCGTCGCCACGGCCGACCCGTCCGACCAGCAGGCGGCCGAGAAGATCAAGTTCGCCTCCCAGATGG
GCGTGGACTGGGTCATCGCCGAGTACGACAAGCTCTCGCGCATGGTCGAGGCCGCCGCCGCGAGCGCGGCCGAAACCATC
AACAGCATCGTCGGTACCGAGTTCGAGTTCGACGACGTCACGGCCGACACCGCCGGCGACGCCAACGAACAGGCTATCGC
CGAGGTCGAGGACGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGCGGTCAGCATGCGCGCCTCGGACATCC
ACTTCGAGCCCTACGAACACCACTACCGCGTGCGCTTCCGGGTCGACGGCGAACTGCGCGAGGTCGCGAGCCCGCCCACC
GTGATCAAGGACAAGCTGGCGTCGCGCATCAAGGTCATCTCGCGGCTCGACATCTCCGAGAAGCGCGTGCCCCAGGACGG
GCGCATGAAGCTCAAGATCGGCCCCGACCGCGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGGTCCGGATTCTCGACCCCAGCAGCGCGCGCATGGGCATCGATGCGCTGGGCTACGACGCCGACGAGAAGGAACGC
CTGCTGCACGCGATCAGCCGGCCCTATGGCATGGTGCTCGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCGGGCGTGAACATCGCGACCGCCGAAGACCCGTCGGAAATCAACCTGCCGGGCG
TCAACCAGGTCAACGTGAACGAGAAGGCCGGCCTGACCTTCGCGGCCGCGCTGCGGGCCTTCCTGCGGCAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCT
GTCGACGCTGCACACCAACGACGCGCCCACCACCCTCACGCGGATGCGCAACATGGGCATCGCGCCCTTCAACATCGCAT
CGAGCGTGATCCTGATAACCGCCCAGCGGCTCGCGCGGCGACTGTGCATGACCTGCCGCACGCCCGCGGACATCCCGCGC
GAGGCGCTGATCGAGGCCGGTTTCCAGGAGCACGAGGTCGACGGCAGCTGGAAGCCCTACCGGCCCGTGGGCTGCGCGGC
CTGCACCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCCGAGGCGATCCAGGAGATCATCCTGC
GCGACGGCAGCGCGCTCGACATCGCGCAGCAGTCGGAAGCCGAAGGCGTGCGCTCGCTGCGCAAGTCGGGCTTGCGCAAG
GTGATGCAGGGCCTGACCTCGCTCGAGGAAGTGGTGGCGGTCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-156)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.545

97.227

0.53
  pilB Acinetobacter baylyi ADP1

53.263

98.267

0.523
  pilF Neisseria gonorrhoeae MS11

52.669

97.4

0.513
  pilB Legionella pneumophila strain ERS1305867

48.759

97.747

0.477
  pilB Vibrio campbellii strain DS40M4

45.745

97.747

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.907

97.4

0.447
  pilB Vibrio cholerae strain A1552

45.567

97.747

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.643

91.681

0.373
  pilF Thermus thermophilus HB27

37.566

98.267

0.369

Multiple sequence alignment