Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEI37_RS24860 Genome accession   NZ_CP168303
Coordinates   2942827..2944551 (+) Length   574 a.a.
NCBI ID   WP_373378572.1    Uniprot ID   -
Organism   Cupriavidus nantongensis strain HB4B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2937827..2949551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEI37_RS24825 (ACEI37_24825) obgE 2938387..2939484 (-) 1098 WP_062801793.1 GTPase ObgE -
  ACEI37_RS24830 (ACEI37_24830) rpmA 2939598..2939858 (-) 261 WP_012353931.1 50S ribosomal protein L27 -
  ACEI37_RS24835 (ACEI37_24835) rplU 2939898..2940209 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  ACEI37_RS24840 (ACEI37_24840) ispB 2940691..2941620 (+) 930 WP_092306243.1 octaprenyl diphosphate synthase -
  ACEI37_RS24850 (ACEI37_24850) - 2941865..2942185 (-) 321 WP_373378570.1 helix-turn-helix domain-containing protein -
  ACEI37_RS24855 (ACEI37_24855) - 2942166..2942582 (-) 417 WP_373378571.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ACEI37_RS24860 (ACEI37_24860) pilF 2942827..2944551 (+) 1725 WP_373378572.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEI37_RS24865 (ACEI37_24865) - 2944617..2945888 (+) 1272 WP_025583719.1 type II secretion system F family protein -
  ACEI37_RS24870 (ACEI37_24870) pilD 2945892..2946791 (+) 900 WP_373378573.1 A24 family peptidase Machinery gene
  ACEI37_RS24875 (ACEI37_24875) coaE 2946875..2947501 (+) 627 WP_373378574.1 dephospho-CoA kinase -
  ACEI37_RS24880 (ACEI37_24880) zapD 2947721..2948479 (+) 759 WP_373378575.1 cell division protein ZapD -
  ACEI37_RS24885 (ACEI37_24885) - 2948497..2948685 (+) 189 WP_373378576.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63299.92 Da        Isoelectric Point: 7.0955

>NTDB_id=1043784 ACEI37_RS24860 WP_373378572.1 2942827..2944551(+) (pilF) [Cupriavidus nantongensis strain HB4B5]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLAQYNLSKVPPALAGNREF
HAHRLLPLGRRDNRLVLALSDPSNQAGLDAIRDKYKLPVEAVVVEHDKLMKHVRAAGEALGTLKNISPVQAERKMIEYDP
VAAAANPRNRTTADNIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDKDAKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFATALRSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAAFNIASSVLMITAQRLARRLCSCKREGEIPREALL
EAGFREHDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQEIILAHGTALQIAEQARKDGVLSLREAGLLKVRQ
GVTSLEEVLATTNT

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1043784 ACEI37_RS24860 WP_373378572.1 2942827..2944551(+) (pilF) [Cupriavidus nantongensis strain HB4B5]
ATGACACTCGGACTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCGCGTGA
AAAGCAATCGCAGCTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCGCTGTTTGCCGCCGACA
AGTACCAGCTGCCGCTGCTGGACCTGGCCCAGTACAACCTTTCCAAGGTGCCGCCGGCGCTGGCCGGCAACCGTGAATTC
CATGCCCACCGGCTGCTGCCGCTGGGCCGGCGCGACAACCGCCTGGTGCTGGCGCTGTCCGATCCGTCCAACCAGGCCGG
CCTGGACGCGATCCGCGACAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCATGTGC
GCGCCGCCGGCGAGGCGCTGGGCACGCTGAAGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGATCCG
GTCGCCGCCGCCGCCAACCCGCGCAACCGCACCACGGCCGACAACATCGACGATGCCCCGGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAGGCCTTCCACCGCGGCGCCTCGGACCTGCACTTCGAACCGTTCGAAACCTTCTACCGCATCCGCTTTC
GTGTCGATGGCGTGCTGCAGGAGGTCGCGCGCCCGCCGCTGGATATCCGCGACAAGATCGCTACCCGGATCAAGGTGCTG
TCGCGGCTGGATATCTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGACAAGGA
TGCCAAGGAAACCGTCGAGAAGGCGGTGGACTTCCGCGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGC
GGATCCTGGAATCGTCGTCCGACAAGCTCGACATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGGCGCTGCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCAGCGGCAAGACCGTGTCGCTGTACACCTTCCT
GAACCTGCTGAACCAGGGCGACATCAATATCTCGACGGCCGAGGATCCTGCCGAAATCCAGCTGCCGGGCATCAACCAGG
TCAACGTCAACGACAAGGCCGGGCTGACCTTCGCCACCGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAGATCCGCGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCACAGACCGGCCACCTGGTGTTGTCGACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCCGCCTTCAATATTGCGTCGAGCGTGC
TGATGATCACGGCGCAGCGGCTGGCGCGGCGCCTGTGCAGCTGCAAGCGCGAGGGCGAGATCCCGCGCGAGGCCTTGCTG
GAAGCGGGCTTCCGCGAGCATGACCTGGACGGCAGCTGGCAGCCCTACCACCCGGTCGGCTGCGAGCGCTGCAACGGCAG
CGGCTACAAGGGCCGCTGCGGCATCTACCAGGTGATGCCGATCACCGAGGCCATGCAGGAGATCATCCTGGCGCACGGCA
CCGCGCTACAGATTGCCGAGCAGGCGCGCAAGGACGGCGTGCTATCGTTGCGCGAAGCGGGGTTGCTGAAGGTCAGGCAG
GGCGTCACGTCACTCGAAGAAGTGCTGGCGACCACGAACACGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

51.813

100

0.523
  pilB Acinetobacter baumannii D1279779

52.091

100

0.521
  pilB Acinetobacter baylyi ADP1

52.269

95.993

0.502
  pilB Legionella pneumophila strain ERS1305867

50.091

95.993

0.481
  pilB Vibrio cholerae strain A1552

45.391

100

0.455
  pilB Vibrio campbellii strain DS40M4

45.841

94.251

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

94.251

0.429
  pilF Thermus thermophilus HB27

38.687

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.312

96.167

0.378

Multiple sequence alignment