Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEN2W_RS22595 Genome accession   NZ_CP168246
Coordinates   2944200..2944706 (-) Length   168 a.a.
NCBI ID   WP_014617872.1    Uniprot ID   F6G3Z0
Organism   Ralstonia solanacearum strain 071-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2921344..2945495 2944200..2944706 within 0


Gene organization within MGE regions


Location: 2921344..2945495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN2W_RS22500 (ACEN2W_22490) - 2921344..2922105 (+) 762 WP_014617860.1 tyrosine-type recombinase/integrase -
  ACEN2W_RS22505 (ACEN2W_22495) - 2922153..2923363 (+) 1211 WP_086002887.1 IS3 family transposase -
  ACEN2W_RS22510 (ACEN2W_22500) - 2923329..2925011 (-) 1683 WP_014617868.1 AAA family ATPase -
  ACEN2W_RS22515 (ACEN2W_22505) - 2925011..2925694 (-) 684 WP_124994723.1 hypothetical protein -
  ACEN2W_RS22520 (ACEN2W_22510) - 2925806..2926570 (-) 765 WP_042567705.1 hypothetical protein -
  ACEN2W_RS22525 (ACEN2W_22515) - 2926624..2927190 (-) 567 WP_042567706.1 recombinase family protein -
  ACEN2W_RS22530 (ACEN2W_22520) - 2927444..2928106 (+) 663 WP_014617864.1 hypothetical protein -
  ACEN2W_RS22535 (ACEN2W_22525) - 2928109..2929311 (+) 1203 WP_014617863.1 ImmA/IrrE family metallo-endopeptidase -
  ACEN2W_RS22540 (ACEN2W_22530) - 2929928..2934919 (-) 4992 WP_014617862.1 type ISP restriction/modification enzyme -
  ACEN2W_RS22545 (ACEN2W_22535) - 2935082..2935660 (-) 579 WP_124994724.1 hypothetical protein -
  ACEN2W_RS22555 (ACEN2W_22545) - 2936978..2937700 (-) 723 WP_086002895.1 HNH endonuclease -
  ACEN2W_RS22565 (ACEN2W_22555) - 2939035..2939274 (-) 240 WP_014617869.1 hypothetical protein -
  ACEN2W_RS22570 (ACEN2W_22560) istA 2939673..2940689 (+) 1017 WP_039548595.1 IS21 family transposase -
  ACEN2W_RS22575 (ACEN2W_22565) istB 2940686..2941474 (+) 789 WP_014615828.1 IS21-like element ISRso19 family helper ATPase IstB -
  ACEN2W_RS22580 (ACEN2W_22570) - 2941526..2941819 (-) 294 WP_346824833.1 hypothetical protein -
  ACEN2W_RS22585 (ACEN2W_22575) moaC 2942201..2942302 (+) 102 Protein_2708 cyclic pyranopterin monophosphate synthase MoaC -
  ACEN2W_RS22590 (ACEN2W_22580) - 2942379..2944115 (-) 1737 WP_014617871.1 Wzy polymerase domain-containing protein -
  ACEN2W_RS22595 (ACEN2W_22585) pilA 2944200..2944706 (-) 507 WP_014617872.1 pilin Machinery gene
  ACEN2W_RS22600 (ACEN2W_22590) pilA 2944974..2945495 (-) 522 WP_014617873.1 pilin Machinery gene

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 16882.51 Da        Isoelectric Point: 9.9749

>NTDB_id=1043440 ACEN2W_RS22595 WP_014617872.1 2944200..2944706(-) (pilA) [Ralstonia solanacearum strain 071-2]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAANAQSDLSVGSSTFA
ATKNVSGLAINATAAPAGQITITYTTAAGNGTLALVPTSGGNALVVSSAPGAAIMWTCYSAGKATAASSVAASPAATLLP
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1043440 ACEN2W_RS22595 WP_014617872.1 2944200..2944706(-) (pilA) [Ralstonia solanacearum strain 071-2]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACCATTCGTGCACGCGTGACCGAAGGTCTGTCGCTGG
CTGCGCAGGCCAAGTCGCTGGTGGCTGAGAATGCTGCAAACGCTCAGAGCGACCTGTCTGTGGGCTCGTCGACGTTTGCT
GCTACCAAGAACGTGTCCGGCCTTGCTATTAACGCGACTGCGGCACCGGCCGGCCAAATCACGATTACGTACACCACTGC
AGCGGGTAACGGTACGCTTGCCCTTGTCCCGACTTCGGGCGGTAATGCTTTGGTCGTGAGTTCCGCTCCGGGCGCAGCTA
TCATGTGGACCTGCTATTCTGCTGGTAAGGCCACGGCTGCCAGCAGCGTTGCTGCGAGCCCCGCGGCGACCCTGCTGCCG
AAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(44-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6G3Z0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

79.167

100

0.792
  pilA2 Legionella pneumophila str. Paris

52.174

95.833

0.5
  pilA2 Legionella pneumophila strain ERS1305867

52.174

95.833

0.5
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.265

100

0.446
  comP Acinetobacter baylyi ADP1

42.353

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.095

100

0.363

Multiple sequence alignment