Detailed information NTG1043323

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACEN3H_RS13840
• Genome accession: NZ_CP168231
• Coordinates: 2971957..2973075 (+)
• Length: 372 a.a.
• NCBI ID: WP_031968809.1
• Uniprot ID: A0A829S4W4
• Organism: Acinetobacter lactucae strain 2024CK-01095
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2966957..2978075

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEN3H_RS13830 (ACEN3H_13830)	-	2970080..2970772 (-)	693	WP_373357915.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACEN3H_RS13835 (ACEN3H_13835)	pilT	2970899..2971936 (+)	1038	WP_081175033.1	type IV pilus twitching motility protein PilT	Machinery gene
ACEN3H_RS13840 (ACEN3H_13840)	pilU	2971957..2973075 (+)	1119	WP_031968809.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACEN3H_RS13845 (ACEN3H_13845)	fur	2973145..2973582 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
ACEN3H_RS13850 (ACEN3H_13850)	-	2973694..2974092 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
ACEN3H_RS13855 (ACEN3H_13855)	-	2974157..2974471 (-)	315	WP_016144536.1	RnfH family protein	-
ACEN3H_RS13860 (ACEN3H_13860)	-	2974471..2975538 (-)	1068	WP_373357917.1	hypothetical protein	-
ACEN3H_RS13865 (ACEN3H_13865)	-	2975692..2976147 (-)	456	WP_002121698.1	bacteriohemerythrin	-
ACEN3H_RS13870 (ACEN3H_13870)	-	2976399..2977247 (-)	849	WP_016144533.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.7278

>NTDB_id=1043323 ACEN3H_RS13840 WP_031968809.1 2971957..2973075(+) (pilU) [Acinetobacter lactucae strain 2024CK-01095]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIREIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1043323 ACEN3H_RS13840 WP_031968809.1 2971957..2973075(+) (pilU) [Acinetobacter lactucae strain 2024CK-01095]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGTGAAATTATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGAGATAAATCTGCC
CGTTTTCGTGTGAGTGCTTTTCAGCAACGTGACATGCCGGGCATGGTATTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTACAACTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGTATCATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACCTCATTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATTATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGTTCTCGTGAAGTAATGGACT
ACGCAATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTTGATCGT
ATTATTCACTTCTTTGAAAGTGATCGTCACAGCCAGCTCTACATGGACCTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCAACACCTGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCGTGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCGCTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGATTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A829S4W4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363