Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB9L16_RS01670 Genome accession   NZ_CP168138
Coordinates   324012..326099 (+) Length   695 a.a.
NCBI ID   WP_056787342.1    Uniprot ID   -
Organism   Pseudomonas sp. LW8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 319012..331099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9L16_RS01650 (AB9L16_01650) pilM 321227..322291 (+) 1065 WP_056787331.1 pilus assembly protein PilM Machinery gene
  AB9L16_RS01655 (AB9L16_01655) - 322291..322857 (+) 567 WP_056787334.1 PilN domain-containing protein -
  AB9L16_RS01660 (AB9L16_01660) pilO 322854..323477 (+) 624 WP_041477570.1 type 4a pilus biogenesis protein PilO -
  AB9L16_RS01665 (AB9L16_01665) - 323474..323998 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  AB9L16_RS01670 (AB9L16_01670) pilQ 324012..326099 (+) 2088 WP_056787342.1 type IV pilus secretin PilQ Machinery gene
  AB9L16_RS01675 (AB9L16_01675) aroK 326104..326622 (+) 519 WP_024011270.1 shikimate kinase AroK -
  AB9L16_RS01680 (AB9L16_01680) aroB 326775..327875 (+) 1101 WP_073471604.1 3-dehydroquinate synthase -
  AB9L16_RS01685 (AB9L16_01685) - 327886..329472 (+) 1587 WP_041477574.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75138.44 Da        Isoelectric Point: 5.4106

>NTDB_id=1042492 AB9L16_RS01670 WP_056787342.1 324012..326099(+) (pilQ) [Pseudomonas sp. LW8]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQMANKNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAPRAATAATAAAAPARPAAQRSR
AIRGVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVE
PSGTFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AEAKVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNAS
GVNGSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1042492 AB9L16_RS01670 WP_056787342.1 324012..326099(+) (pilQ) [Pseudomonas sp. LW8]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCACCGGCACGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGATGGCCAACAAGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAGTT
GGCGCCTTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGACTGCGCCGCGCC
CGGCTGCCGTTGCACCGCGTGCCGCCACGGCCGCCACGGCCGCTGCGGCACCTGCCAGACCAGCCGCGCAACGCAGCAGG
GCGATTCGCGGCGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAATGTGGTCATCGATCTCTCTGATCCGACCATCGC
CCCGGATATCCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAACTGCCGGAAAAACTGCGCGTGCGCC
TCGACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGTACGGTGATCAGTGTCGAG
CCCAGCGGCACCTTCGACTATTCCACATTCCAGACCGACAACAAGCTCACCGTGAGCGTTCGTCCGATGAGCATCGACGA
CCTGCAAAAGCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGCT
CGGTGCTGCAACTGATCGCGGACTTCACCAATCTCAACCTGGTGGCGAGCGACACGGTGCAGGGCGGCATCACGTTGCGT
CTGCAAAACGTGCCGTGGGATCAGGCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGT
GCTGCTGGTCGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGG
CGCCCCTGCGTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATTGCCAAGCTGTTCCAGTCGGTGACCAGT
GCCGAGGCCAAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGA
TCGCCTCGACGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGCATCGTTG
AGGCCAACGTCGATTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCCTCC
GGGGTCAACGGTTCGTCGAGCACTATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGT
CAATAACACTTCGGGGATCGGCATTGCTTTCATCACCGACAACGTACTGCTGGATCTCGAACTGACGGCGATGGAAAAAA
CCGGCAACGGCGAAATCGTCTCACAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAG
ATTCCCTATCAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTCACCCC
GCAGATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGATTACCTGAACAAAGTGCAGG
ATGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTT
TTTTCAAATACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.688

100

0.74
  comQ Acinetobacter baylyi ADP1

41.32

100

0.414
  pilQ Acinetobacter baumannii strain A118

37.366

100

0.4
  pilQ Acinetobacter baumannii D1279779

37.366

100

0.4
  pilQ Legionella pneumophila strain Lp02

37.83

98.129

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.794

97.842

0.37

Multiple sequence alignment