Detailed information NTG1041563

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACDZ80_RS13500
• Genome accession: NZ_CP168027
• Coordinates: 2715729..2716343 (-)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CM35 isolate
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2710729..2721343

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACDZ80_RS13470 (ACDZ80_13470)	rpsT	2711029..2711295 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACDZ80_RS13475 (ACDZ80_13475)	holA	2711312..2712353 (-)	1042	Protein_2611	DNA polymerase III subunit delta	-
ACDZ80_RS13480 (ACDZ80_13480)	-	2712393..2712547 (-)	155	Protein_2612	hypothetical protein	-
ACDZ80_RS13485 (ACDZ80_13485)	-	2712588..2712722 (+)	135	WP_003152870.1	YqzM family protein	-
ACDZ80_RS13490 (ACDZ80_13490)	comEC	2712741..2715092 (-)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACDZ80_RS13495 (ACDZ80_13495)	-	2715093..2715662 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACDZ80_RS13500 (ACDZ80_13500)	comEA	2715729..2716343 (-)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACDZ80_RS13505 (ACDZ80_13505)	comER	2716402..2717223 (+)	822	WP_015417855.1	late competence protein ComER	-
ACDZ80_RS13510 (ACDZ80_13510)	-	2717292..2718029 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
ACDZ80_RS13515 (ACDZ80_13515)	rsfS	2718026..2718382 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACDZ80_RS13520 (ACDZ80_13520)	yqeK	2718400..2718960 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACDZ80_RS13525 (ACDZ80_13525)	-	2718950..2719519 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACDZ80_RS13530 (ACDZ80_13530)	yhbY	2719530..2719820 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACDZ80_RS13535 (ACDZ80_13535)	aroE	2719814..2720656 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=1041563 ACDZ80_RS13500 WP_053573180.1 2715729..2716343(-) (comEA) [Bacillus velezensis strain CM35 isolate]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1041563 ACDZ80_RS13500 WP_053573180.1 2715729..2716343(-) (comEA) [Bacillus velezensis strain CM35 isolate]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363