Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDH69_RS15690 Genome accession   NZ_CP167866
Coordinates   3647142..3648878 (-) Length   578 a.a.
NCBI ID   WP_338339702.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:581     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3646192..3656075 3647142..3648878 within 0


Gene organization within MGE regions


Location: 3646192..3656075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDH69_RS15690 (ACDH69_15690) pilB 3647142..3648878 (-) 1737 WP_338339702.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDH69_RS15695 (ACDH69_15695) galE 3648995..3649972 (-) 978 WP_372359089.1 UDP-glucose 4-epimerase GalE -
  ACDH69_RS15700 (ACDH69_15700) - 3650061..3651605 (-) 1545 WP_372359090.1 NAD(P)/FAD-dependent oxidoreductase -
  ACDH69_RS15705 (ACDH69_15705) - 3651598..3652068 (-) 471 WP_228317397.1 GtrA family protein -
  ACDH69_RS15710 (ACDH69_15710) - 3652071..3653972 (-) 1902 WP_230430545.1 hypothetical protein -
  ACDH69_RS15715 (ACDH69_15715) pilE 3654056..3654463 (-) 408 WP_372359091.1 pilin Machinery gene
  ACDH69_RS15720 (ACDH69_15720) pilC 3654816..3656075 (+) 1260 WP_228317394.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62440.55 Da        Isoelectric Point: 5.5986

>NTDB_id=1040027 ACDH69_RS15690 WP_338339702.1 3647142..3648878(-) (pilB) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:581]
MNAVSTANLVGITGLARRLVQDGALDEAVARDAMEKAASAKLPLPIWLAQNKVVGAAQLAAANAVEFGMPLLDVSAFDAS
QSAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1040027 ACDH69_RS15690 WP_338339702.1 3647142..3648878(-) (pilB) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:581]
ATGAACGCCGTCAGTACTGCCAACCTTGTCGGAATCACTGGTCTAGCGCGCCGCTTGGTCCAAGATGGAGCGCTGGACGA
AGCCGTCGCTCGCGACGCGATGGAGAAAGCGGCTTCAGCCAAGCTGCCGCTCCCCATCTGGCTTGCCCAGAACAAAGTTG
TAGGCGCGGCACAGTTAGCCGCAGCCAACGCGGTTGAGTTCGGTATGCCTTTGCTCGACGTATCAGCATTCGACGCCAGC
CAGAGTGCCGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAGGCGA
TCCTCGTTGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAAATGGGCGACCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACACCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGACTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTATGGCATGGTATTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATCCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCAGCGTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTTGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCCGAAGGATTCACGCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGCGTGACAAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.613

100

0.557
  pilB Acinetobacter baylyi ADP1

55.44

100

0.555
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilF Neisseria gonorrhoeae MS11

49.648

98.27

0.488
  pilB Vibrio cholerae strain A1552

50.832

93.599

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

46.29

97.924

0.453
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.065

92.561

0.362

Multiple sequence alignment