Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCQ01_RS16925 Genome accession   NZ_CP167840
Coordinates   3817778..3819511 (-) Length   577 a.a.
NCBI ID   WP_228431843.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 4037     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3812778..3824511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ01_RS16910 (ACCQ01_16910) - 3814153..3815763 (+) 1611 WP_228431845.1 PAS domain-containing sensor histidine kinase -
  ACCQ01_RS16915 (ACCQ01_16915) pilR 3815972..3817366 (+) 1395 WP_228431844.1 sigma-54 dependent transcriptional regulator Regulator
  ACCQ01_RS16925 (ACCQ01_16925) pilB 3817778..3819511 (-) 1734 WP_228431843.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCQ01_RS16930 (ACCQ01_16930) - 3819615..3820253 (-) 639 WP_228431842.1 methionine biosynthesis protein MetW -
  ACCQ01_RS16935 (ACCQ01_16935) - 3820246..3821292 (-) 1047 WP_081390738.1 glycosyltransferase family 2 protein -
  ACCQ01_RS16940 (ACCQ01_16940) - 3821289..3822083 (-) 795 WP_076057322.1 glycosyltransferase family A protein -
  ACCQ01_RS16945 (ACCQ01_16945) - 3822088..3822468 (-) 381 WP_228431841.1 SMR family transporter -
  ACCQ01_RS16950 (ACCQ01_16950) - 3822813..3823229 (+) 417 WP_228431840.1 pilin -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62857.00 Da        Isoelectric Point: 5.4508

>NTDB_id=1039749 ACCQ01_RS16925 WP_228431843.1 3817778..3819511(-) (pilB) [Xanthomonas sp. NCPPB 4037]
MNSVVTANLVGITGIARRLVQDGALDEGAARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANSVEFGMPLLDVSSFDPN
QNAIKLVSEELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDDEMGDLDVSTGDEEMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1039749 ACCQ01_RS16925 WP_228431843.1 3817778..3819511(-) (pilB) [Xanthomonas sp. NCPPB 4037]
ATGAATAGCGTCGTCACCGCCAACTTGGTTGGCATCACGGGCATTGCCCGGCGGCTTGTGCAGGACGGTGCTCTGGATGA
AGGCGCGGCGCGTGCTGCGATGGATCAGGCCGCTACTGCCAAGGTCCCGTTGCCGCAATGGTTCTCAGAGAAGAAGCTGG
TGACCGCTTCGCAATTAGCAGCTGCCAACTCAGTGGAGTTCGGGATGCCGCTACTTGATGTGTCATCGTTCGACCCTAAT
CAGAATGCAATCAAGTTGGTTAGCGAAGAACTGCTGCAAAAGTTTCAAGTGCTGCCCCTGTTCAAGCGCGGTAATCGGTT
GTTTGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTTCACACCAATTTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACATTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCTTCGATGGGC
GACGACGATGATGAAATGGGGGATCTGGACGTCTCCACCGGGGACGAGGAGATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCCTATGAAGATGATTACCGGGTGCGGTTGCGGATCGATGGGTTGTTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGTTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTTCGCGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGTAAGACGGTGTCGTT
GTACACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATTCGCTTGCCTG
GTGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACATTTGCCGCTGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGCTGCACACCAACGATGCGCCGCAGACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCA
CCTCCTCGGTGACCCTGGTCATCGCGCAGCGTTTGGCACGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCCGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAAGGCCGTACCGGCATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACTTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.44

0.548
  pilB Acinetobacter baylyi ADP1

55.634

98.44

0.548
  pilB Legionella pneumophila strain ERS1305867

51.408

98.44

0.506
  pilF Neisseria gonorrhoeae MS11

48.944

98.44

0.482
  pilB Vibrio cholerae strain A1552

49.546

95.494

0.473
  pilB Vibrio campbellii strain DS40M4

45.47

99.48

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.826

97.574

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

35.738

100

0.378

Multiple sequence alignment