Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABRP17_RS13025 Genome accession   NZ_CP167826
Coordinates   3010148..3011872 (+) Length   574 a.a.
NCBI ID   WP_188326367.1    Uniprot ID   A0A7H1AP68
Organism   Stenotrophomonas sp. WHRI 8082     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3005148..3016872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRP17_RS13020 (ABRP17_013020) - 3006414..3009461 (+) 3048 WP_349985727.1 fused MFS/spermidine synthase -
  ABRP17_RS13025 (ABRP17_013025) pilB 3010148..3011872 (+) 1725 WP_188326367.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABRP17_RS13030 (ABRP17_013030) - 3012039..3012746 (+) 708 WP_192093984.1 YitT family protein -
  ABRP17_RS13035 (ABRP17_013035) - 3012956..3013492 (-) 537 WP_349985726.1 DNA-3-methyladenine glycosylase I -
  ABRP17_RS13040 (ABRP17_013040) - 3013489..3015285 (-) 1797 WP_349985725.1 DUF4153 domain-containing protein -
  ABRP17_RS13045 (ABRP17_013045) - 3015367..3015933 (+) 567 WP_188326371.1 YqgE/AlgH family protein -
  ABRP17_RS13050 (ABRP17_013050) ruvX 3015926..3016393 (+) 468 WP_188326372.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62336.37 Da        Isoelectric Point: 6.0417

>NTDB_id=1039645 ABRP17_RS13025 WP_188326367.1 3010148..3011872(+) (pilB) [Stenotrophomonas sp. WHRI 8082]
MNAVATANLVGITGIARRLVQDGALDEAGARDAMAKATAARLPLPQWFAQNKVVTASQLAAANALEFGMPLFDVSVFDAN
QSAISLVSEELLRKHHVLPLFKRGGKLYVGTSNPTHSLDEIKFHTNLVVESILVDDDQIRRALEQWQTSHDSMTSSMGDD
DEGMANLEIGTDDDMAASDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKLN
QRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQKLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRRTDLPAHAL
LAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEISTIILAGGNALQIADAAQAIGVNDLRQSALIKAGK
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1039645 ABRP17_RS13025 WP_188326367.1 3010148..3011872(+) (pilB) [Stenotrophomonas sp. WHRI 8082]
ATGAACGCAGTTGCCACCGCTAACCTCGTCGGTATCACCGGCATCGCGCGCCGTCTGGTGCAGGACGGTGCGTTGGATGA
AGCCGGCGCCCGTGATGCGATGGCCAAGGCGACCGCTGCGCGGTTGCCGCTGCCGCAATGGTTCGCCCAGAACAAGGTCG
TCACCGCCTCGCAGCTCGCCGCGGCCAACGCGCTCGAGTTCGGCATGCCGCTGTTCGATGTCAGCGTGTTCGACGCCAAC
CAGAGCGCGATCAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCATCACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTACGTTGGCACCAGCAATCCCACCCATTCGCTGGACGAAATCAAGTTCCACACCAACCTGGTGGTGGAGTCGATCCTGG
TCGATGACGATCAGATCCGCCGGGCGCTGGAGCAGTGGCAGACCAGCCACGATTCGATGACCTCGTCCATGGGCGACGAC
GACGAGGGCATGGCGAACCTCGAGATCGGCACCGACGACGACATGGCGGCCAGCGACTCGGGCATCGACGCCAAGGGCGA
CGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCAATCCGCAAGGGCGCATCGGATATCCATTTCGAGC
CGTACGAAGATGACTATCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTGGCGCGTGCGCCGGTCAAGTTGAAC
CAGCGCATCGCGGCGCGCCTGAAGGTCATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGATGGGCGCATCAA
GCTCAACCTGAGCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCCACGTTGTTCGGTGAAAAGGTGGTGCTGC
GTATCCTGGACGGCAGTGCGGCCAAGCTGGGTATCGACAAGCTGGGCTATGAGGCCGATCAGCAGAAACTGTTCCTGGAC
GCCATCCACAAGCCGTACGGCATGGTGCTGGTGACGGGACCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGCGCT
GGGCATCTTGAACGATGAAACGCGCAATATCTCCACGGCCGAAGATCCGGTGGAAATCCGCCTGCCCGGCGTGAACCAGG
TGCAGCAGAACAACAAGCGCGGCATGACGTTTGCCGCCGCGCTGCGTTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCCACGCT
GCATACCAATGACGCGCCGCAGACCATCGCACGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGTTCGGTCA
CGCTGGTGATCGCCCAGCGCCTGGCGCGTCGCCTGTGCAACAACTGCAAGCGCCGCACCGACCTGCCGGCGCATGCCTTG
CTGGCCGAGGGTTTCACCCAGGCACAGCTGGATGCGGGCATCGAACTGTATGAAGCCGTCGGCTGTGATGAGTGCACCGA
AGGCTACAAGGGCCGTACCGGCCTGTATCAGGTGATGCCGATGACCGACGAGATCTCGACGATCATCCTGGCTGGCGGCA
ACGCGCTGCAGATTGCCGATGCCGCCCAGGCGATCGGCGTGAATGACCTGCGCCAGTCCGCGTTGATCAAGGCTGGCAAG
GGCGTGACCAGCCTGGCTGAGATCAACCGCGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H1AP68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.459

100

0.557
  pilB Acinetobacter baylyi ADP1

55.124

98.606

0.544
  pilB Legionella pneumophila strain ERS1305867

53.439

98.78

0.528
  pilF Neisseria gonorrhoeae MS11

48.94

98.606

0.483
  pilB Vibrio cholerae strain A1552

49.265

94.774

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

46.057

97.213

0.448
  pilB Vibrio campbellii strain DS40M4

44.658

99.477

0.444
  pilB/pilB1 Synechocystis sp. PCC 6803

35.256

100

0.383

Multiple sequence alignment