Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCQ28_RS16100 Genome accession   NZ_CP167233
Coordinates   3703009..3704745 (-) Length   578 a.a.
NCBI ID   WP_022558667.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. tamarindi strain NCPPB 584     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3698009..3709745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ28_RS16085 (ACCQ28_16085) sucC 3698051..3699220 (-) 1170 WP_007965356.1 ADP-forming succinate--CoA ligase subunit beta -
  ACCQ28_RS16090 (ACCQ28_16090) - 3699452..3701065 (+) 1614 WP_277573647.1 PAS domain-containing sensor histidine kinase -
  ACCQ28_RS16095 (ACCQ28_16095) pilR 3701333..3702787 (+) 1455 WP_011348337.1 sigma-54 dependent transcriptional regulator Regulator
  ACCQ28_RS16100 (ACCQ28_16100) pilB 3703009..3704745 (-) 1737 WP_022558667.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCQ28_RS16105 (ACCQ28_16105) - 3704809..3705243 (-) 435 WP_078590286.1 pilin -
  ACCQ28_RS16110 (ACCQ28_16110) pilE 3705321..3705761 (-) 441 WP_078590287.1 pilin Machinery gene
  ACCQ28_RS16115 (ACCQ28_16115) pilC 3706088..3707344 (+) 1257 WP_057685777.1 type II secretion system F family protein Machinery gene
  ACCQ28_RS16120 (ACCQ28_16120) - 3707351..3708214 (+) 864 WP_008576868.1 A24 family peptidase -
  ACCQ28_RS16125 (ACCQ28_16125) coaE 3708228..3708833 (+) 606 WP_372183618.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62578.67 Da        Isoelectric Point: 5.6006

>NTDB_id=1039058 ACCQ28_RS16100 WP_022558667.1 3703009..3704745(-) (pilB) [Xanthomonas axonopodis pv. tamarindi strain NCPPB 584]
MSVLSTANLVGITGIARRLVQDGALEESAARSAMDQAALAKVPLPQWFSEKKLVAASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1039058 ACCQ28_RS16100 WP_022558667.1 3703009..3704745(-) (pilB) [Xanthomonas axonopodis pv. tamarindi strain NCPPB 584]
ATGAGCGTTTTATCGACCGCAAATCTCGTTGGAATCACCGGAATTGCCCGCAGGCTGGTGCAGGATGGCGCATTGGAGGA
AAGCGCGGCTCGAAGCGCGATGGATCAGGCTGCTCTTGCGAAAGTTCCACTGCCTCAGTGGTTCTCGGAGAAAAAGCTTG
TTGCTGCCTCGCAGCTGGCGGCGGCCAATGCAGTCGAGTTCGGCATGCCACTACTGGACGTATCGGCATTCGACGCCAGC
CAGAACGCAGTAAAGCTCGTCAGTGAAGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAGGCGA
TCCTCGTCGACGAAGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCAGCGTTGCGCTCGTTCCTACGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCCGAAGGATTCACCCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGGAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.959

100

0.561
  pilB Acinetobacter baylyi ADP1

54.75

100

0.548
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

48.873

99.827

0.488
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

96.886

0.455
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45

Multiple sequence alignment