Detailed information NTG1038738

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACBW56_RS01340
• Genome accession: NZ_CP167196
• Coordinates: 265592..266206 (+)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain FRI-TD24
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 260592..271206

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACBW56_RS01305 (ACBW56_01305)	aroE	261279..262121 (+)	843	WP_025852995.1	shikimate dehydrogenase	-
ACBW56_RS01310 (ACBW56_01310)	yhbY	262115..262405 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACBW56_RS01315 (ACBW56_01315)	-	262416..262985 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACBW56_RS01320 (ACBW56_01320)	yqeK	262975..263556 (+)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACBW56_RS01325 (ACBW56_01325)	rsfS	263553..263909 (+)	357	WP_003152864.1	ribosome silencing factor	-
ACBW56_RS01330 (ACBW56_01330)	-	263906..264643 (+)	738	WP_025852989.1	class I SAM-dependent methyltransferase	-
ACBW56_RS01335 (ACBW56_01335)	comER	264712..265533 (-)	822	WP_014305449.1	late competence protein ComER	-
ACBW56_RS01340 (ACBW56_01340)	comEA	265592..266206 (+)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACBW56_RS01345 (ACBW56_01345)	-	266273..266842 (+)	570	WP_003152868.1	ComE operon protein 2	-
ACBW56_RS01350 (ACBW56_01350)	comEC	266843..269194 (+)	2352	WP_208557679.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACBW56_RS01355 (ACBW56_01355)	-	269213..269347 (-)	135	WP_003152870.1	YqzM family protein	-
ACBW56_RS01360 (ACBW56_01360)	-	269388..269542 (+)	155	Protein_268	hypothetical protein	-
ACBW56_RS01365 (ACBW56_01365)	holA	269582..270623 (+)	1042	Protein_269	DNA polymerase III subunit delta	-
ACBW56_RS01370 (ACBW56_01370)	rpsT	270640..270906 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=1038738 ACBW56_RS01340 WP_003152867.1 265592..266206(+) (comEA) [Bacillus velezensis strain FRI-TD24]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1038738 ACBW56_RS01340 WP_003152867.1 265592..266206(+) (comEA) [Bacillus velezensis strain FRI-TD24]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363