Detailed information NTG1036638

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB8617_RS05365
• Genome accession: NZ_CP166995
• Coordinates: 1141310..1142464 (-)
• Length: 384 a.a.
• NCBI ID: WP_409440065.1
• Uniprot ID: -
• Organism: Psychromonas sp. GE-S-Ul-11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1136310..1147464

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB8617_RS05320 (AB8617_05320)	-	1136965..1137699 (+)	735	WP_290038885.1	class I SAM-dependent methyltransferase	-
AB8617_RS05325 (AB8617_05325)	rnhA	1137696..1138157 (-)	462	WP_290038884.1	ribonuclease HI	-
AB8617_RS05330 (AB8617_05330)	dnaQ	1138250..1138966 (+)	717	WP_290038883.1	DNA polymerase III subunit epsilon	-
AB8617_RS05335 (AB8617_05335)	-	1138957..1140255 (+)	1299	WP_409440064.1	hypothetical protein	-
AB8617_RS05365 (AB8617_05365)	pilU	1141310..1142464 (-)	1155	WP_409440065.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB8617_RS05370 (AB8617_05370)	-	1143009..1143272 (+)	264	WP_290038880.1	DUF4212 domain-containing protein	-
AB8617_RS05375 (AB8617_05375)	-	1143287..1145005 (+)	1719	WP_290038879.1	sodium:solute symporter family protein	-
AB8617_RS05380 (AB8617_05380)	-	1145144..1146988 (+)	1845	WP_409440066.1	DUF294 nucleotidyltransferase-like domain-containing protein	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 42671.97 Da
• Isoelectric Point: 6.1936

>NTDB_id=1036638 AB8617_RS05365 WP_409440065.1 1141310..1142464(-) (pilU) [Psychromonas sp. GE-S-Ul-11]
MEFNAMLSQLSEQDGSDLYLATGAPPSAKFQGELIPLSDVSFKPGDVKAIAYDIMDPEQIEEFEHTLEMNLAISIGGIGR
FRVNIFKQRNDVSIVVRNIKINIPKFADLRLPEVLKKVVMAKNGLILFVGATGSGKSTSLAALIDHRNENSSGHIVTIED
PVEFVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDTETMEHALAFSETGHLVLSTLHANNANQAIDRI
INFFTEERRAQLLMDLGNNLRAFVSQRLIPTIDGKRCAAIEVMMGTLTIRDMIKRGHFESLKEVMEKSKELGMQTFDSAL
FDLVKEGLISEQEAIKNSDSPTNLALKFKLDKGSFAEPEVKPKSKSSLSLEPKQLGDINDLTYS

Nucleotide

• Length: 1155 bp

>NTDB_id=1036638 AB8617_RS05365 WP_409440065.1 1141310..1142464(-) (pilU) [Psychromonas sp. GE-S-Ul-11]
ATGGAATTTAATGCAATGTTAAGTCAATTATCAGAACAAGATGGTTCTGATCTTTATTTGGCCACAGGAGCACCTCCTTC
AGCTAAATTTCAAGGTGAGTTAATTCCCTTAAGTGATGTTTCTTTTAAACCAGGTGATGTTAAGGCGATAGCCTACGACA
TTATGGATCCAGAACAAATTGAAGAGTTTGAACATACCTTAGAAATGAATCTGGCGATTTCTATCGGTGGAATAGGGCGA
TTTCGAGTTAATATTTTTAAACAGCGAAATGACGTGTCAATCGTCGTGCGAAACATTAAAATTAACATTCCTAAGTTTGC
TGACTTAAGATTACCTGAAGTACTAAAAAAAGTGGTGATGGCTAAAAATGGTTTGATACTATTTGTCGGAGCGACTGGTT
CAGGTAAATCGACTTCACTAGCAGCCTTAATTGACCATCGCAATGAAAATTCATCCGGCCATATTGTCACTATCGAGGAT
CCTGTTGAGTTCGTTCATCGTCATAAAAAAAGTATTATTAACCAAAGAGAAGTTGGCGTTGATACGCGTAGCTTTCATGC
CGCACTTAAGAATACCCTAAGACAGGCACCAGACGTTATTCTAATTGGTGAAATACGCGATACAGAAACTATGGAGCATG
CATTAGCCTTTTCTGAGACTGGGCACCTAGTGCTTTCTACGTTACATGCCAATAATGCTAACCAAGCGATTGACCGTATC
ATTAACTTCTTTACAGAGGAAAGACGTGCTCAGTTGTTAATGGACCTAGGTAATAATTTAAGAGCCTTTGTTTCGCAACG
TTTAATTCCCACAATTGATGGCAAACGTTGTGCTGCGATTGAAGTGATGATGGGAACATTAACCATTCGAGACATGATTA
AGCGTGGACATTTCGAAAGCTTAAAAGAGGTAATGGAAAAGTCTAAAGAATTAGGTATGCAAACTTTTGATAGCGCTCTG
TTTGATTTGGTGAAAGAAGGTTTAATTAGCGAACAAGAAGCGATTAAAAACTCCGACTCTCCTACCAACTTAGCACTTAA
GTTTAAACTTGATAAAGGCAGTTTTGCAGAGCCGGAAGTGAAGCCAAAAAGTAAAAGTAGTTTAAGTTTAGAGCCGAAAC
AGCTAGGGGACATTAACGACTTAACTTATTCGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (59-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.226	96.875	0.516
pilU	Acinetobacter baylyi ADP1	53.541	91.927	0.492
pilU	Vibrio cholerae strain A1552	48.588	92.188	0.448
pilT	Pseudomonas aeruginosa PAK	40.988	89.583	0.367
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.056	88.802	0.365
pilT	Pseudomonas stutzeri DSM 10701	40.407	89.583	0.362