Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAY00_RS11195 Genome accession   NZ_CP166961
Coordinates   2501658..2503352 (+) Length   564 a.a.
NCBI ID   WP_371373517.1    Uniprot ID   -
Organism   Thalassotalea aquiviva strain 273M-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2496658..2508352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAY00_RS11170 ampE 2496761..2497612 (-) 852 WP_371373503.1 regulatory signaling modulator protein AmpE -
  ACAY00_RS11175 ampD 2497750..2498349 (-) 600 WP_371373505.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACAY00_RS11180 - 2498395..2498913 (+) 519 WP_371373508.1 TIGR02281 family clan AA aspartic protease -
  ACAY00_RS11185 nadC 2499017..2499892 (+) 876 WP_371373511.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACAY00_RS11190 - 2500933..2501397 (+) 465 WP_371373514.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACAY00_RS11195 pilB 2501658..2503352 (+) 1695 WP_371373517.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAY00_RS11200 pilC 2503369..2504613 (+) 1245 WP_371373520.1 type II secretion system F family protein Machinery gene
  ACAY00_RS11205 pilD 2504663..2505562 (+) 900 WP_371373523.1 prepilin peptidase Machinery gene
  ACAY00_RS11210 coaE 2505568..2506173 (+) 606 WP_371373527.1 dephospho-CoA kinase -
  ACAY00_RS11215 - 2506311..2507057 (+) 747 WP_371373530.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62280.14 Da        Isoelectric Point: 4.8043

>NTDB_id=1036342 ACAY00_RS11195 WP_371373517.1 2501658..2503352(+) (pilB) [Thalassotalea aquiviva strain 273M-4]
MNSLGSQSSILSALQRNELLPDDTLNEFNKAREEKSIIKFLLRKKAISSNRLATVLAQSFGLPLVNINDLELDNLPDKLL
NENLIQKHHALPIYLRGNMLYIAVSDPTQLEVLQEFQFNTGFSCELVLADANDLQSTIELLFDETSSLELTDLDEEELKQ
IDTNDGKQDDDEGAENPENDAPIVVYINKILLDAIRRGASDLHFEPFEHKFRIRFRIDGILTEIATPPVLLSSRLTARLK
VMSRLDIAERRVPQDGRIKLAVSKKKSIDFRVSTLPTMWGEKVVMRILDSSSAELGIDILGYEDEQKALYLEALSKPQGM
ILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVQINNKAGLTFSSALKSFLRQDPDIVMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPSYNVASSVSLIIAQRLARRLCNHCKAKDEITEQGLIEFGFDPS
EVSDITIYKPVGCSKCTNGFKGRVGIYEVVKISDVTAGLIMRGANSIDIAKQAQSEGYNDLRQSALLKAKAGITSLSEVI
RVTA

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1036342 ACAY00_RS11195 WP_371373517.1 2501658..2503352(+) (pilB) [Thalassotalea aquiviva strain 273M-4]
ATGAACAGTTTAGGATCGCAATCCAGTATCTTAAGTGCATTACAACGAAATGAGTTGTTACCCGATGACACCTTGAATGA
ATTTAACAAAGCCCGTGAAGAAAAGTCGATCATCAAGTTTTTACTTCGTAAAAAAGCCATTTCATCGAATCGCTTGGCAA
CTGTTTTAGCACAAAGCTTTGGCTTACCTCTTGTTAATATCAACGACCTTGAGCTTGATAATTTACCCGATAAACTTCTC
AACGAAAACCTGATTCAAAAGCATCATGCCCTGCCCATTTATTTAAGAGGGAATATGCTCTATATTGCGGTCTCCGATCC
GACCCAGTTAGAGGTATTACAAGAGTTTCAATTTAACACCGGATTCTCTTGTGAACTGGTTTTAGCCGATGCTAATGATC
TGCAATCGACTATAGAGCTGCTATTCGATGAAACCAGTTCGTTAGAGCTCACCGATTTAGATGAAGAAGAGCTTAAGCAA
ATCGATACCAACGATGGCAAGCAAGATGATGATGAGGGCGCTGAAAACCCCGAAAACGATGCGCCGATTGTTGTTTACAT
CAATAAAATCTTACTCGATGCGATCCGCCGTGGTGCCTCCGATTTACATTTTGAACCGTTTGAGCATAAATTTCGCATCC
GCTTTCGAATTGACGGTATTCTTACCGAAATCGCCACACCGCCGGTATTGCTTTCAAGCCGGTTAACGGCTCGTTTAAAG
GTTATGTCAAGGCTAGATATTGCTGAACGTCGAGTGCCACAAGATGGTCGCATAAAGTTAGCGGTATCAAAGAAAAAGTC
GATCGACTTTCGTGTCTCTACCCTACCAACCATGTGGGGCGAAAAAGTGGTAATGCGTATATTAGACTCGTCTTCAGCCG
AGCTCGGCATTGATATTCTGGGTTATGAAGATGAGCAAAAAGCGCTTTATTTAGAAGCCCTGTCTAAACCGCAAGGGATG
ATATTAGTAACCGGCCCCACAGGCTCGGGTAAAACCGTATCACTTTATACCGGTCTTAATATTTTAAATACCGAAGAGCG
CAATATCTCGACCGCCGAAGATCCGGTAGAGATTAACCTCGAAGGGATAAATCAGGTTCAAATCAATAATAAAGCCGGCC
TAACCTTTTCCAGCGCGCTAAAATCTTTCTTACGTCAAGATCCTGATATTGTTATGGTTGGTGAGATCCGAGATTTAGAA
ACCGCCGAGATAGCAATTAAAGCGGCGCAAACAGGTCACTTGGTTTTATCAACCCTGCATACCAATTCAGCGCCAGAAAC
CTTAACCCGTTTACTGAATATGGGCGTGCCCAGTTATAACGTCGCCAGCTCGGTCTCGTTAATAATTGCGCAGCGTCTTG
CTCGTCGATTATGCAATCATTGTAAAGCTAAGGATGAGATAACCGAGCAAGGCTTAATCGAATTTGGCTTCGACCCCAGT
GAAGTTAGCGATATCACCATTTATAAGCCGGTTGGTTGCAGTAAATGCACCAATGGTTTTAAAGGTCGAGTCGGTATTTA
TGAAGTGGTCAAAATCAGTGATGTCACCGCAGGCTTAATCATGAGAGGCGCCAATTCTATTGATATCGCTAAACAAGCGC
AAAGTGAAGGCTATAACGATTTGCGTCAATCCGCTTTATTAAAAGCCAAAGCTGGGATCACCAGTTTGTCCGAAGTAATT
AGGGTTACGGCCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-146)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.435

95.39

0.548
  pilB Acinetobacter baumannii D1279779

56.611

95.213

0.539
  pilB Acinetobacter baylyi ADP1

55.473

95.567

0.53
  pilB Vibrio cholerae strain A1552

54.206

94.858

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

53.962

93.972

0.507
  pilB Vibrio campbellii strain DS40M4

53.484

94.149

0.504
  pilF Neisseria gonorrhoeae MS11

50.746

95.035

0.482

Multiple sequence alignment