Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACAY00_RS11205 Genome accession   NZ_CP166961
Coordinates   2504663..2505562 (+) Length   299 a.a.
NCBI ID   WP_371373523.1    Uniprot ID   -
Organism   Thalassotalea aquiviva strain 273M-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2499663..2510562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAY00_RS11190 - 2500933..2501397 (+) 465 WP_371373514.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACAY00_RS11195 pilB 2501658..2503352 (+) 1695 WP_371373517.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAY00_RS11200 pilC 2503369..2504613 (+) 1245 WP_371373520.1 type II secretion system F family protein Machinery gene
  ACAY00_RS11205 pilD 2504663..2505562 (+) 900 WP_371373523.1 prepilin peptidase Machinery gene
  ACAY00_RS11210 coaE 2505568..2506173 (+) 606 WP_371373527.1 dephospho-CoA kinase -
  ACAY00_RS11215 - 2506311..2507057 (+) 747 WP_371373530.1 cell division protein ZapD -
  ACAY00_RS11220 - 2507068..2508849 (-) 1782 WP_371373533.1 Wzy polymerase domain-containing protein -
  ACAY00_RS11225 - 2508887..2509975 (-) 1089 WP_371379711.1 Wzy polymerase domain-containing protein -
  ACAY00_RS11230 - 2510054..2510299 (-) 246 Protein_2133 O-antigen polymerase -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 33298.01 Da        Isoelectric Point: 8.2262

>NTDB_id=1036344 ACAY00_RS11205 WP_371373523.1 2504663..2505562(+) (pilD) [Thalassotalea aquiviva strain 273M-4]
MFAEILALFELYPITFYTTTLILGLVVGSFLNVVIYRLPKMLHNEWLCDCRELLADELAKTSNKASSKAKIESITLSKPA
STCPKCGHKIKAIENIPLLSWLFLKGKCSACKTPISIRYPLVELASGILSVVIGLHFGVTWLTLWMLILTWSLVALTMID
FDQMILPDQITLPLVWLGLLINLNSGFVSLNEAVIGAVVGYMSLWSIFWLFKLLTGKEGMGYGDFKLFAVFGAWFGWQLL
PLLILMASLVGAIIGIAMMVFNNHQGSKPIPFGPYLAIAGWITALWGNGMWNWYLNLVL

Nucleotide


Download         Length: 900 bp        

>NTDB_id=1036344 ACAY00_RS11205 WP_371373523.1 2504663..2505562(+) (pilD) [Thalassotalea aquiviva strain 273M-4]
GTGTTTGCTGAAATTTTGGCTCTATTTGAGCTCTACCCGATTACGTTTTATACAACCACCCTAATACTCGGTTTAGTTGT
TGGTAGCTTTTTAAATGTGGTGATTTATCGTTTGCCCAAAATGCTCCACAACGAGTGGCTGTGTGATTGTCGCGAACTGC
TTGCGGATGAGTTAGCTAAAACCAGTAACAAAGCAAGCTCTAAAGCTAAAATAGAGTCGATAACCTTGTCCAAACCCGCT
TCGACTTGCCCAAAGTGTGGCCATAAAATCAAAGCAATCGAAAACATCCCGCTGTTATCCTGGCTGTTTTTAAAAGGCAA
ATGTTCGGCTTGCAAAACACCAATTTCGATACGATATCCTTTGGTTGAACTGGCTAGCGGTATTTTATCTGTGGTTATTG
GCCTGCACTTTGGTGTCACTTGGTTAACGCTATGGATGTTAATCTTAACTTGGTCTTTGGTTGCTCTAACCATGATTGAC
TTTGACCAGATGATCCTGCCCGATCAAATTACACTGCCCTTAGTTTGGCTTGGTTTACTGATCAACTTAAATTCGGGATT
TGTCAGTCTTAATGAAGCTGTAATAGGCGCTGTGGTCGGCTATATGAGCTTATGGTCCATTTTTTGGCTCTTTAAGTTAC
TTACAGGCAAAGAAGGTATGGGCTATGGTGACTTTAAGCTGTTTGCGGTGTTTGGCGCATGGTTTGGCTGGCAACTGTTA
CCTTTGCTCATTTTAATGGCTTCGCTAGTTGGGGCAATTATTGGTATCGCTATGATGGTTTTCAACAATCACCAAGGCTC
TAAACCCATTCCATTTGGTCCTTACTTAGCCATTGCCGGTTGGATTACCGCTCTATGGGGTAATGGTATGTGGAACTGGT
ATTTAAATTTGGTGTTATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(148-256)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.195

97.993

0.502
  pilD Vibrio campbellii strain DS40M4

48.475

98.662

0.478
  pilD Acinetobacter nosocomialis M2

48.288

97.659

0.472
  pilD Acinetobacter baumannii D1279779

47.945

97.659

0.468
  pilD Neisseria gonorrhoeae MS11

47.842

92.977

0.445

Multiple sequence alignment