Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB1S55_RS08135 Genome accession   NZ_CP166840
Coordinates   1860121..1861824 (-) Length   567 a.a.
NCBI ID   WP_370981309.1    Uniprot ID   -
Organism   Agaribacterium sp. ZY112     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1855121..1866824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1S55_RS08105 (AB1S55_08100) argJ 1855502..1856719 (+) 1218 WP_370981303.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  AB1S55_RS08110 (AB1S55_08105) mutT 1856722..1857111 (+) 390 WP_370981304.1 8-oxo-dGTP diphosphatase MutT -
  AB1S55_RS08115 (AB1S55_08110) - 1857167..1857361 (-) 195 WP_370981305.1 DNA gyrase inhibitor YacG -
  AB1S55_RS08120 (AB1S55_08115) coaE 1857440..1858036 (-) 597 WP_370981306.1 dephospho-CoA kinase -
  AB1S55_RS08125 (AB1S55_08120) pilD 1858036..1858899 (-) 864 WP_370981307.1 A24 family peptidase Machinery gene
  AB1S55_RS08130 (AB1S55_08125) pilC 1858903..1860117 (-) 1215 WP_370981308.1 type II secretion system F family protein Machinery gene
  AB1S55_RS08135 (AB1S55_08130) pilB 1860121..1861824 (-) 1704 WP_370981309.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB1S55_RS08140 (AB1S55_08135) - 1862107..1862640 (+) 534 WP_370981310.1 pilin -
  AB1S55_RS08145 (AB1S55_08140) - 1862807..1863472 (-) 666 WP_370981311.1 hypothetical protein -
  AB1S55_RS08150 (AB1S55_08145) nadC 1863505..1864347 (-) 843 WP_370981312.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB1S55_RS08155 (AB1S55_08150) - 1864370..1864891 (-) 522 WP_370981313.1 TIGR02281 family clan AA aspartic protease -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62001.85 Da        Isoelectric Point: 4.7091

>NTDB_id=1035615 AB1S55_RS08135 WP_370981309.1 1860121..1861824(-) (pilB) [Agaribacterium sp. ZY112]
MILSGLARRLVNDELLTLEQAEELESAAKAKKHPFPIHLAQTSELSALKVAQIASEEFGTPLLDLSALNPDSPPKDLVDS
NLIQQHYALPILKRGNRLYIAVCDPTNLHGLDEIKFNTGLNTEAILVEAEQLQMAIEAYLNAAEDNLGDSFGGLDDNDLD
SLDIEAVDDSSESGDDSSNDDEAPIVRFVNKVLLDAIKSGASDIHFEPYEKVYRVRFRTDGILQEVARPPINLGTRLSAR
LKVMSKMDISERRVPQDGRIKMKITKSRAIDFRVNTLPTLFGEKVVLRILDPSSAKLGIDALGYEDFQKKLFMDALEQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVERNISTAEDPVEINLEGINQVNVSNKVGLTFAEALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNVATTVSLIIAQRLARRLCSECKKPVDLPDEVLKEEGID
DIGLEEIKLHEPVGCKACNNGYKGRVGVYEVVRITPPISRIIMEGGNAIQIADAAKEAGFFNLRTAALRKAAMGVTSLAE
ANRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1035615 AB1S55_RS08135 WP_370981309.1 1860121..1861824(-) (pilB) [Agaribacterium sp. ZY112]
GTGATATTAAGTGGCTTAGCCCGAAGACTTGTAAATGACGAGCTATTAACCTTAGAACAGGCCGAAGAGCTTGAATCTGC
AGCTAAAGCTAAAAAGCATCCTTTTCCTATTCACCTAGCACAAACCAGTGAATTGAGTGCCTTAAAAGTCGCCCAGATAG
CATCGGAAGAATTTGGAACTCCGCTGTTAGACTTAAGCGCACTTAATCCTGACAGCCCGCCTAAAGACCTTGTAGATTCA
AATTTAATTCAACAGCATTACGCACTTCCCATTCTAAAAAGGGGGAATCGTTTGTACATTGCTGTTTGTGACCCCACCAA
TCTCCACGGCCTAGACGAGATTAAATTTAACACTGGACTAAACACTGAAGCCATTCTCGTAGAGGCGGAACAACTTCAAA
TGGCCATTGAGGCCTACCTAAATGCGGCAGAAGACAACCTCGGAGACTCCTTTGGTGGCCTCGACGATAACGACCTAGAC
AGTTTAGATATTGAAGCCGTCGATGACTCATCAGAAAGCGGGGACGACAGCTCAAACGATGATGAGGCGCCAATTGTTCG
GTTCGTCAATAAAGTACTCTTGGATGCTATTAAAAGTGGGGCCTCAGACATTCATTTTGAGCCTTATGAAAAAGTCTATA
GAGTGCGATTTCGTACTGATGGAATATTACAAGAAGTCGCTCGCCCACCAATAAATCTGGGCACCCGCCTTTCTGCTCGC
TTAAAAGTAATGTCTAAAATGGACATATCAGAGCGGCGCGTCCCCCAGGATGGGCGCATCAAAATGAAAATAACCAAAAG
TCGAGCAATTGATTTTCGTGTCAACACCCTACCCACTCTCTTTGGTGAAAAAGTTGTTTTACGAATTCTGGATCCTAGCA
GCGCCAAACTTGGTATTGATGCGCTTGGCTATGAAGACTTTCAAAAGAAACTGTTTATGGATGCTCTAGAGCAGCCACAA
GGTATGATTCTGGTTACTGGACCTACCGGCTCCGGTAAAACGGTCTCCCTCTATACTGGGCTCAACATATTAAATACCGT
TGAGAGAAACATCAGTACAGCTGAAGACCCAGTGGAAATAAATCTCGAAGGAATCAATCAGGTTAACGTTAGCAACAAAG
TAGGGCTAACTTTTGCTGAGGCGCTTCGCTCTTTTCTTCGTCAAGACCCTGATATAGTGATGGTTGGCGAGATTCGAGAC
CTAGAGACTGCCGAAATTGCCATTAAAGCAGCACAAACGGGGCACTTGGTACTATCTACACTGCACACCAATAGTGCCCC
TGAGACCCTTACTCGACTCATGAATATGGGCGTACCTACATTCAATGTAGCGACAACAGTCAGCCTTATTATTGCGCAAC
GCCTAGCGAGACGCCTTTGCTCTGAATGTAAAAAACCAGTAGACCTACCTGACGAAGTGCTAAAGGAAGAGGGCATAGAT
GACATTGGCCTAGAAGAGATTAAACTCCATGAACCTGTTGGCTGTAAAGCATGTAATAACGGCTACAAAGGCCGCGTTGG
TGTTTATGAGGTGGTTCGCATCACCCCTCCTATTAGTCGTATTATAATGGAGGGAGGCAACGCAATTCAGATTGCTGATG
CAGCTAAAGAAGCCGGCTTCTTTAATTTACGAACTGCTGCATTACGAAAAGCAGCTATGGGCGTAACCAGCTTGGCAGAA
GCAAACCGAGTTACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-143)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.825

100

0.601
  pilB Acinetobacter baumannii D1279779

58.979

100

0.591
  pilB Legionella pneumophila strain ERS1305867

55.3

99.824

0.552
  pilB Vibrio cholerae strain A1552

50.697

100

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.159

98.942

0.506
  pilB Vibrio campbellii strain DS40M4

50.796

99.647

0.506
  pilF Neisseria gonorrhoeae MS11

48.944

100

0.49
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.245

93.474

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

41.683

88.007

0.367

Multiple sequence alignment