Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QUE89_RS03480 Genome accession   NZ_AP028070
Coordinates   764569..766632 (+) Length   687 a.a.
NCBI ID   WP_286221855.1    Uniprot ID   -
Organism   Marinobacter sp. LA51     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 759569..771632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE89_RS03460 pilM 761715..762779 (+) 1065 WP_286221851.1 pilus assembly protein PilM Machinery gene
  QUE89_RS03465 - 762779..763345 (+) 567 WP_286221852.1 PilN domain-containing protein -
  QUE89_RS03470 pilO 763342..763953 (+) 612 WP_286221853.1 type 4a pilus biogenesis protein PilO -
  QUE89_RS03475 - 763956..764504 (+) 549 WP_286221854.1 pilus assembly protein PilP -
  QUE89_RS03480 pilQ 764569..766632 (+) 2064 WP_286221855.1 type IV pilus secretin PilQ Machinery gene
  QUE89_RS03485 aroK 766771..767337 (+) 567 WP_286221856.1 shikimate kinase AroK -
  QUE89_RS03490 aroB 767330..768424 (+) 1095 WP_286221857.1 3-dehydroquinate synthase -
  QUE89_RS03495 - 768470..769885 (+) 1416 WP_286221858.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 687 a.a.        Molecular weight: 73741.78 Da        Isoelectric Point: 4.3199

>NTDB_id=103556 QUE89_RS03480 WP_286221855.1 764569..766632(+) (pilQ) [Marinobacter sp. LA51]
MFKKLNVYVGVIAFGLLSGLANAVTLEDVSFSSLPGDRLEVKLAFDGQPPEPTGYTIERPARIAVDLSDTTNGLGSRSIP
LGSGNAQSMTVVETKDRTRLIFNLVELVPYDTVRSGNSLVMTLGGDASGSSSAGSTTSAPTAAPASNRPDALAGVDFRRG
NNGEGRVVVDLGSESTPVDLSELGGKIRLTMSGISVPENLRRRLDVTDFATPVNRIDTFVQDGNAIVEIRPEGNYDYIAY
QSGSQFTVSVEKLTEQEAESRREEKFPYTGEKLSLNFQDIEVRSVLQLIADFTGLNLVASDTVGGSITLRLQNVPWDQAL
DLILKTKGLDKRQIGNVLLVAPADEIAAREKLELETTKQIAELAPVRLDIIQVNYAKAADVVALIEADKELITDRGFVSS
DVRTNTISVRETTEKLEEIRRLVSTWDVPVRQVSIEARIVRAQTNVAEDLGIRWGGAAYDVSGDNVFSVGGSQSAVEEAR
QAAGGTNNTITFPGALAVDLGVTGEGASSFAIGWGSDDFLVDLELSALESDGQAEVVSQPRVVTADRQTASIKSGEEIPY
QEASSSGATSVSFKEAVLSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPSINTNEVTTQVLVGNGETVVLGGIFQSEV
ATQTTKTPFLGDIPYLGRLFKRTEHVDERSELLIFITPKILKNDLIR

Nucleotide


Download         Length: 2064 bp        

>NTDB_id=103556 QUE89_RS03480 WP_286221855.1 764569..766632(+) (pilQ) [Marinobacter sp. LA51]
ATGTTTAAAAAACTCAATGTATACGTCGGCGTGATTGCTTTTGGGTTGTTATCCGGCCTGGCCAACGCGGTCACGCTGGA
AGACGTGTCGTTTTCGTCGCTACCGGGAGATCGTCTGGAGGTGAAACTTGCCTTTGATGGGCAGCCCCCAGAACCGACGG
GATATACGATCGAGCGTCCGGCTCGTATCGCAGTAGACTTGAGCGATACTACTAACGGACTTGGAAGTCGCAGCATTCCG
CTAGGCTCCGGCAATGCTCAGAGCATGACAGTGGTCGAAACCAAGGATCGGACTCGCCTGATCTTTAACCTTGTCGAACT
GGTCCCTTATGACACGGTCCGGAGTGGTAATTCCCTGGTCATGACTCTGGGAGGAGATGCATCCGGGTCATCGTCTGCGG
GCTCGACCACCTCAGCACCGACAGCAGCGCCAGCGTCGAATAGACCGGATGCGCTAGCAGGTGTTGATTTCCGCCGTGGC
AACAACGGAGAAGGTCGGGTTGTAGTCGACTTGGGCAGCGAGAGCACCCCTGTAGACCTGTCTGAGTTGGGTGGGAAAAT
TCGTCTGACCATGTCAGGCATTTCGGTGCCCGAAAACCTCCGTCGTCGGCTGGACGTGACCGACTTTGCGACACCAGTCA
ATCGAATTGACACTTTCGTGCAGGATGGCAATGCCATTGTAGAAATCAGGCCGGAAGGGAACTACGACTACATTGCCTAT
CAGTCTGGCAGTCAGTTCACGGTCAGTGTTGAAAAGCTGACTGAGCAGGAAGCTGAGTCCCGCCGCGAAGAGAAGTTCCC
ATACACGGGTGAGAAGCTCTCTCTCAATTTCCAGGACATTGAAGTTCGCTCCGTGCTCCAGTTGATTGCCGACTTCACCG
GATTGAACCTGGTGGCCAGTGACACTGTTGGTGGAAGCATAACCCTGAGGCTCCAGAATGTTCCCTGGGATCAAGCGCTT
GATCTGATTCTTAAAACCAAGGGTTTGGATAAGCGTCAGATCGGTAACGTACTTCTTGTGGCGCCCGCTGATGAGATCGC
TGCTCGTGAGAAGCTTGAACTGGAAACCACCAAGCAAATTGCCGAACTTGCTCCGGTTCGTCTTGATATCATTCAGGTGA
ATTATGCCAAAGCTGCTGATGTGGTGGCGTTGATTGAAGCGGACAAGGAACTGATTACCGACCGAGGATTTGTCTCTTCG
GACGTGCGCACCAACACTATCAGTGTGCGTGAGACCACTGAAAAGTTGGAAGAAATTCGACGCTTGGTCTCGACCTGGGA
CGTGCCGGTTCGTCAGGTGTCTATAGAAGCTCGTATCGTTCGTGCCCAAACCAACGTGGCTGAAGATCTCGGTATTCGCT
GGGGTGGCGCAGCCTACGATGTGAGCGGCGACAACGTTTTCTCAGTCGGCGGCTCCCAGAGTGCGGTTGAAGAGGCCCGT
CAGGCAGCCGGAGGCACCAATAACACGATCACCTTCCCGGGAGCATTGGCAGTAGATCTCGGTGTAACGGGTGAAGGTGC
GTCTTCATTCGCCATCGGTTGGGGCAGTGACGACTTCCTGGTCGACCTGGAACTCTCCGCATTGGAAAGTGACGGTCAAG
CCGAAGTCGTATCACAGCCGCGAGTAGTGACTGCGGACCGCCAGACAGCGTCGATCAAGTCTGGCGAAGAAATTCCATAC
CAGGAAGCATCTTCAAGCGGTGCCACCTCTGTGTCCTTCAAGGAAGCGGTGTTGTCGCTTGAGGTAACTCCGCAGATCAC
GCCTGATGACAAGATCATCATGGACCTGGTGGTGAATCAGGATTCGCGTGGCGAGGTAACTGCGGGCATTCCGTCAATCA
ACACCAACGAAGTTACAACTCAGGTGCTGGTTGGCAACGGCGAAACCGTAGTGTTGGGCGGTATCTTCCAGTCGGAGGTG
GCCACCCAAACCACCAAGACACCGTTCCTGGGTGATATCCCGTACCTCGGTCGTCTGTTCAAGCGGACTGAGCATGTGGA
TGAGCGCAGTGAGCTGCTGATCTTCATCACGCCCAAGATCCTGAAGAACGATCTGATCCGCTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(296-342)

Bacterial type II and III secretion system protein (Secretin)

(527-682)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

51.227

100

0.517
  comQ Acinetobacter baylyi ADP1

40.935

100

0.421
  pilQ Acinetobacter baumannii D1279779

39.889

100

0.419
  pilQ Acinetobacter baumannii strain A118

39.889

100

0.419
  pilQ Legionella pneumophila strain ERS1305867

41.973

98.836

0.415
  pilQ Legionella pneumophila strain Lp02

41.778

98.253

0.41

Multiple sequence alignment