Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABK045_RS20695 Genome accession   NZ_CP166498
Coordinates   4445503..4447233 (-) Length   576 a.a.
NCBI ID   WP_053506935.1    Uniprot ID   -
Organism   Stenotrophomonas pavanii strain 9978     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4440503..4452233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABK045_RS20680 (ABK045_20695) sucC 4440790..4441959 (-) 1170 WP_005418614.1 ADP-forming succinate--CoA ligase subunit beta -
  ABK045_RS20685 (ABK045_20700) - 4442367..4443980 (+) 1614 WP_049471427.1 PAS domain-containing sensor histidine kinase -
  ABK045_RS20690 (ABK045_20705) pilR 4444035..4445408 (+) 1374 WP_049471425.1 sigma-54 dependent transcriptional regulator Regulator
  ABK045_RS20695 (ABK045_20710) pilB 4445503..4447233 (-) 1731 WP_053506935.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABK045_RS20700 (ABK045_20715) - 4447394..4448227 (-) 834 WP_064239075.1 sterol desaturase family protein -
  ABK045_RS20705 (ABK045_20720) pilA/pilAI 4448422..4448850 (-) 429 WP_149901976.1 pilin Machinery gene
  ABK045_RS20710 (ABK045_20725) pilC 4449205..4450464 (+) 1260 WP_064239077.1 type II secretion system F family protein Machinery gene
  ABK045_RS20715 (ABK045_20730) - 4450468..4451331 (+) 864 WP_064239078.1 A24 family peptidase -
  ABK045_RS20720 (ABK045_20735) coaE 4451343..4451954 (+) 612 WP_064239079.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62227.11 Da        Isoelectric Point: 5.8722

>NTDB_id=1034803 ABK045_RS20695 WP_053506935.1 4445503..4447233(-) (pilB) [Stenotrophomonas pavanii strain 9978]
MNTVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGIGNLDVSGGDEDISGGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPANLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=1034803 ABK045_RS20695 WP_053506935.1 4445503..4447233(-) (pilB) [Stenotrophomonas pavanii strain 9978]
ATGAACACCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGCGCCCTGGACGA
GGCTTCTGCCCGTGACGCCATGGCCAAGGCCGCTGCGGCCCGCCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TCGGGGCGGCCCAGTTGGCGGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCAATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAATCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCGCTGGGCGGTGAC
GACGATGGCATTGGCAACCTGGATGTGTCCGGCGGTGACGAGGACATTTCCGGGGGCGGTGACTCCGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGACGACTACCGCGTGCGCCTGCGCATTGATGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTGAAG
CTGAACCAGCGTATCGCCGCACGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGGTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGACGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACGCGTAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCTTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCACTGCGCTCGTTCCTGCGACAGGACCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCCCAGACCGGCCACATGGTGCTGTC
CACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATTGCGCCGTACAACATCACCAGTT
CGGTGACGCTGGTGATTGCCCAGCGCCTGGCGCGCCGGTTGTGCAACAACTGCAAGCGCCCGGCCAACCTGCCGGAGCAC
GCGCTGCTGGCTGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGTATCGAACTGTACGAGGCGGTCGGCTGTGACGAATG
CACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGCTGGCCG
GTGGCAATGCGTTGCAGATTGCCGAGGCCGCGCAGCAGATCGGGGTGAACGACCTGCGCCAGTCGGCGTTGAAGAAGGCG
GCGGCCGGGATCACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.611

0.549
  pilB Acinetobacter baylyi ADP1

54.799

99.479

0.545
  pilB Legionella pneumophila strain ERS1305867

52.021

98.785

0.514
  pilF Neisseria gonorrhoeae MS11

48.944

98.611

0.483
  pilB Vibrio cholerae strain A1552

48.601

99.306

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.306

0.453
  pilB Vibrio campbellii strain DS40M4

45.518

98.785

0.45

Multiple sequence alignment