Detailed information NTG1034069

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB6A22_RS12310
• Genome accession: NZ_CP166297
• Coordinates: 2587524..2588138 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain FCW119-1M2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2582524..2593138

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB6A22_RS12280 (AB6A22_12300)	rpsT	2582824..2583090 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB6A22_RS12285 (AB6A22_12305)	holA	2583107..2584148 (-)	1042	Protein_2372	DNA polymerase III subunit delta	-
AB6A22_RS12290 (AB6A22_12310)	-	2584188..2584342 (-)	155	Protein_2373	hypothetical protein	-
AB6A22_RS12295 (AB6A22_12315)	-	2584383..2584517 (+)	135	WP_003152870.1	YqzM family protein	-
AB6A22_RS12300 (AB6A22_12320)	comEC	2584536..2586887 (-)	2352	WP_239819197.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB6A22_RS12305 (AB6A22_12325)	-	2586888..2587457 (-)	570	WP_003152868.1	ComE operon protein 2	-
AB6A22_RS12310 (AB6A22_12330)	comEA	2587524..2588138 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB6A22_RS12315 (AB6A22_12335)	comER	2588197..2589018 (+)	822	WP_115996552.1	late competence protein ComER	-
AB6A22_RS12320 (AB6A22_12340)	-	2589087..2589824 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
AB6A22_RS12325 (AB6A22_12345)	rsfS	2589821..2590177 (-)	357	WP_239819198.1	ribosome silencing factor	-
AB6A22_RS12330 (AB6A22_12350)	yqeK	2590174..2590755 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB6A22_RS12335 (AB6A22_12355)	-	2590745..2591314 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
AB6A22_RS12340 (AB6A22_12360)	yhbY	2591325..2591615 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AB6A22_RS12345 (AB6A22_12365)	aroE	2591609..2592451 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=1034069 AB6A22_RS12310 WP_043867297.1 2587524..2588138(-) (comEA) [Bacillus velezensis strain FCW119-1M2]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1034069 AB6A22_RS12310 WP_043867297.1 2587524..2588138(-) (comEA) [Bacillus velezensis strain FCW119-1M2]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363