Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB8599_RS13655 Genome accession   NZ_CP166194
Coordinates   3070705..3072399 (+) Length   564 a.a.
NCBI ID   WP_407331441.1    Uniprot ID   -
Organism   Enterovibrio sp. 27052020O     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3065705..3077399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8599_RS13635 (AB8599_13640) pdhR 3067288..3068061 (-) 774 WP_407331437.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AB8599_RS13640 (AB8599_13645) ampD 3068362..3068913 (-) 552 WP_407331438.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB8599_RS13645 (AB8599_13650) nadC 3069108..3070013 (+) 906 WP_407331439.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB8599_RS13650 (AB8599_13655) - 3070244..3070681 (+) 438 WP_407331440.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB8599_RS13655 (AB8599_13660) pilB 3070705..3072399 (+) 1695 WP_407331441.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8599_RS13660 (AB8599_13665) pilC 3072411..3073634 (+) 1224 WP_407331442.1 type II secretion system F family protein Machinery gene
  AB8599_RS13665 (AB8599_13670) pilD 3073774..3074646 (+) 873 WP_407331443.1 prepilin peptidase Machinery gene
  AB8599_RS13670 (AB8599_13675) coaE 3074647..3075267 (+) 621 WP_407331444.1 dephospho-CoA kinase -
  AB8599_RS13675 (AB8599_13680) zapD 3075260..3076000 (+) 741 WP_407331445.1 cell division protein ZapD -
  AB8599_RS13680 (AB8599_13685) yacG 3076070..3076222 (+) 153 WP_407333228.1 DNA gyrase inhibitor YacG -
  AB8599_RS13685 (AB8599_13690) mutT 3076310..3076714 (-) 405 WP_407331446.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61296.89 Da        Isoelectric Point: 5.1517

>NTDB_id=1033642 AB8599_RS13655 WP_407331441.1 3070705..3072399(+) (pilB) [Enterovibrio sp. 27052020O]
MASSLVQLLKSHGFLNAELESILNREVIEAGESPASFIIRSGVYSSSELANLLSSIFSEPILNLQSVSKLPLNLAAISHS
LVLSTDSLPIEVNGKELILAISDPTNLEAQDEFRFATGLQIKPVLVDHQQLVGAIRKLYGKQIASGQGDAKSVSDSELAD
LAEFDDDANSDDLATDEAPVSRYIQQVLMDAVRKQASDIHFEPFEQRYQIRFRLDGILQTHGTPPVGISRRLSTRLKILA
KLNIAERRLPQDGRIKLTLADGKSIDMRVSTLPTLFGEKVVLRILDGGSVALDIDSLGYSEIQKQDYLTALDKPQGLILM
TGPTGSGKTVSLYTGLKYLNTAERNISTAEDPVEINLPGINQVNINNDIKLDFATALRAFLRQDPDVVMVGEMRDLETAE
IGIKAAQTGHLVLSTLHTNSAAESITRLANMGVAPYNLAASLTLIIAQRLARRLCQHCKVPHEVDDHQLTALAPLYPSLV
LNHVSQANSEGCDHCNNGYIGRVGIYEVMRIDRTLSEAIGNGASASQLENIARNNGMLTLSLSGIEKLNQGITSLAELQR
VISL

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1033642 AB8599_RS13655 WP_407331441.1 3070705..3072399(+) (pilB) [Enterovibrio sp. 27052020O]
TTGGCTAGTAGTTTAGTTCAGTTATTGAAATCTCACGGTTTCCTGAATGCTGAATTAGAAAGCATCCTAAACCGTGAGGT
GATAGAAGCTGGAGAGTCTCCAGCTTCTTTTATAATTCGTTCAGGAGTATACTCATCATCAGAATTAGCAAATTTGTTAA
GTTCCATTTTTAGCGAACCAATTCTAAATTTGCAAAGTGTATCCAAACTACCATTAAATTTGGCAGCAATCAGCCATAGC
CTGGTACTCAGTACGGATTCTCTTCCGATTGAAGTTAATGGCAAAGAGCTAATTCTTGCTATTAGCGACCCGACAAACCT
TGAAGCTCAAGATGAATTCCGCTTCGCTACTGGCCTGCAAATCAAACCCGTTCTGGTAGACCACCAACAACTGGTGGGCG
CGATTCGCAAACTTTATGGTAAGCAGATAGCATCAGGCCAAGGCGATGCAAAATCCGTCAGTGATTCGGAACTCGCTGAT
CTTGCCGAGTTCGATGACGATGCGAATTCCGATGACCTGGCCACCGACGAAGCCCCGGTTAGCCGCTATATTCAACAAGT
GCTAATGGACGCTGTGCGCAAGCAAGCGTCAGATATTCATTTCGAACCTTTTGAGCAACGCTACCAAATTCGTTTTCGTT
TAGACGGTATATTGCAAACCCACGGTACGCCCCCAGTGGGCATTTCACGTCGCCTTTCGACTCGTTTGAAAATCCTCGCC
AAGCTCAATATTGCTGAGCGCCGATTGCCACAAGATGGGCGGATTAAACTAACGCTGGCGGACGGCAAATCTATCGACAT
GCGCGTATCCACTTTACCAACCTTGTTTGGTGAAAAGGTGGTGCTGCGAATTTTGGATGGCGGCAGTGTCGCGCTGGACA
TTGATTCGCTGGGTTATAGCGAAATACAAAAACAAGATTACCTAACGGCGCTGGATAAACCCCAAGGGCTGATCTTAATG
ACTGGCCCAACCGGTAGCGGCAAAACCGTGTCGCTGTACACCGGCCTGAAGTATCTCAACACTGCCGAACGCAACATATC
CACAGCGGAAGATCCGGTAGAAATCAACTTGCCCGGCATCAATCAGGTCAATATCAATAACGACATCAAGCTCGATTTCG
CCACGGCGCTTCGTGCTTTTTTACGACAAGATCCTGACGTGGTGATGGTCGGTGAGATGCGCGATCTTGAAACCGCTGAA
ATTGGCATCAAGGCCGCACAAACTGGGCACTTGGTGTTATCAACGCTGCATACTAACTCGGCGGCAGAAAGCATTACCCG
CCTAGCGAATATGGGCGTCGCACCTTATAACCTCGCGGCATCACTGACATTGATCATCGCCCAGCGTCTGGCGCGTCGTT
TATGCCAACACTGTAAAGTGCCTCACGAAGTCGATGATCACCAACTGACCGCTTTGGCGCCGCTCTACCCCAGCTTAGTG
TTGAATCACGTTTCTCAAGCTAACTCGGAAGGATGTGACCATTGCAATAATGGCTATATTGGCCGAGTGGGCATTTACGA
GGTGATGCGTATCGATCGCACGCTATCGGAAGCCATTGGTAACGGCGCATCGGCTTCGCAACTTGAAAATATTGCCCGCA
ATAATGGCATGCTCACGCTCTCGCTTTCCGGTATTGAAAAACTCAATCAAGGCATCACCAGCCTCGCAGAACTTCAACGC
GTTATTAGTCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(186-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

58.304

100

0.585
  pilB Vibrio campbellii strain DS40M4

56.294

100

0.571
  pilB Vibrio cholerae strain A1552

56.437

100

0.567
  pilB Acinetobacter baumannii D1279779

48.352

96.809

0.468
  pilB Acinetobacter baylyi ADP1

45.534

100

0.461
  pilB Legionella pneumophila strain ERS1305867

46.904

94.504

0.443
  pilF Neisseria gonorrhoeae MS11

44.007

94.681

0.417

Multiple sequence alignment