Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB8810_RS06155 Genome accession   NZ_CP166096
Coordinates   1425991..1427730 (+) Length   579 a.a.
NCBI ID   WP_369915848.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 3005     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1420991..1432730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8810_RS06135 (AB8810_06140) - 1421370..1422533 (+) 1164 WP_369915844.1 glycosyltransferase -
  AB8810_RS06140 (AB8810_06145) - 1422508..1423758 (-) 1251 WP_369915845.1 lipopolysaccharide biosynthesis protein -
  AB8810_RS06145 (AB8810_06150) - 1423755..1424957 (-) 1203 WP_369915846.1 glycosyltransferase -
  AB8810_RS06150 (AB8810_06155) - 1424981..1425838 (-) 858 WP_369915847.1 glycosyltransferase family 2 protein -
  AB8810_RS06155 (AB8810_06160) pilB 1425991..1427730 (+) 1740 WP_369915848.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8810_RS06160 (AB8810_06165) pilR 1427892..1429280 (-) 1389 WP_369915849.1 sigma-54-dependent transcriptional regulator Regulator
  AB8810_RS06165 (AB8810_06170) - 1429355..1430968 (-) 1614 WP_191824385.1 PAS domain-containing sensor histidine kinase -
  AB8810_RS06170 (AB8810_06175) sucC 1431248..1432411 (+) 1164 WP_191824384.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62780.97 Da        Isoelectric Point: 5.7951

>NTDB_id=1033352 AB8810_RS06155 WP_369915848.1 1425991..1427730(+) (pilB) [Xanthomonas sp. NCPPB 3005]
MNAAVSANLVGITGIARRLVQDGALEEAAARTAMAHAAEAKIPLPQWFSEKKLVTAAQLAAANAVEFGMPLLDVSVFDAS
QSAIKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDTIGNSLG
GDDEGMDNLEVGAGDEDMGGGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRLAPAM
PENALLKEGFTEAEIAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIGAIVLQGGNAMDIAEAAQKIGVKDLRQSAL
LKARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=1033352 AB8810_RS06155 WP_369915848.1 1425991..1427730(+) (pilB) [Xanthomonas sp. NCPPB 3005]
ATGAACGCAGCTGTCTCGGCCAACCTAGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGACGGCGCGCTAGAGGA
AGCTGCCGCGCGGACCGCCATGGCGCATGCCGCCGAGGCCAAGATTCCGCTGCCGCAGTGGTTCTCGGAGAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGCTGGATGTGTCCGTGTTCGACGCCAGC
CAGAGCGCGATCAAGCTGGTCAGCGAGGAGTTGTTGCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTGGGGGTCAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTGACCGTGGAACCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCGAGCAACGATACGATCGGCAACTCGCTGGGC
GGCGACGACGAGGGGATGGACAACCTCGAGGTCGGCGCCGGCGACGAGGACATGGGCGGCGGCGGCGATAGTGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGGTGCTGGTGGATGCGATTCGCCGCGGCGCTTCGGACA
TCCACTTCGAGCCGTACGAGGACGACTATCGGGTGCGGCTGCGCATCGATGGCCTGCTCAAGAGCGTGGCCAAGGCGCCG
GTCAAGCTCAATCAGCGCATCGCGGCGCGGTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
TGGGCGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGACTTCCGCGTCAGTACGCTGCCGACGCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACGGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACTGCCGAGGATCCGGTCGAAATCCGCCTGCCCG
GGGTCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCTTACAACATCA
CCAGCTCGGTGACGCTGGTGATCGCACAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGGCTGGCCCCGGCCATG
CCGGAGAACGCGCTGTTGAAGGAAGGCTTCACCGAGGCCGAAATCGCCGCCGGCATCCAGCTGTACGAGGCGGTTGGCTG
CGACGAATGCACGGAGGGCTACAAGGGCCGCACCGGCATCTACCAGGTGATGCCGATGAATGACGAGATCGGGGCGATCG
TGCTGCAGGGCGGCAATGCGATGGACATCGCCGAGGCGGCGCAGAAGATCGGCGTCAAGGACCTGCGCCAGTCGGCACTG
CTCAAGGCGCGCAACGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.323

98.964

0.547
  pilB Acinetobacter baylyi ADP1

54.912

98.446

0.541
  pilB Legionella pneumophila strain ERS1305867

52.548

98.273

0.516
  pilB Vibrio cholerae strain A1552

49.826

99.482

0.496
  pilF Neisseria gonorrhoeae MS11

48.944

98.1

0.48
  pilB Vibrio campbellii strain DS40M4

46.14

98.446

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.702

96.891

0.453
  pilF Thermus thermophilus HB27

40.684

90.846

0.37

Multiple sequence alignment