Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB8P72_RS00370 Genome accession   NZ_CP166085
Coordinates   81121..82755 (-) Length   544 a.a.
NCBI ID   WP_369957881.1    Uniprot ID   -
Organism   Psychrobacter sp. CLB018     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 76121..87755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8P72_RS00345 (AB8P72_00345) - 76511..76846 (+) 336 WP_068407914.1 pyrimidine/purine nucleoside phosphorylase -
  AB8P72_RS00350 (AB8P72_00350) rlmB 77211..77981 (+) 771 WP_068407911.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AB8P72_RS00355 (AB8P72_00355) coaE 77944..78633 (-) 690 WP_068407908.1 dephospho-CoA kinase -
  AB8P72_RS00360 (AB8P72_00360) pilD 78750..79649 (-) 900 WP_068407907.1 A24 family peptidase Machinery gene
  AB8P72_RS00365 (AB8P72_00365) - 79675..80898 (-) 1224 Protein_72 type II secretion system F family protein -
  AB8P72_RS00370 (AB8P72_00370) pilB 81121..82755 (-) 1635 WP_369957881.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8P72_RS00375 (AB8P72_00375) tpiA 83220..84020 (+) 801 WP_082894377.1 triose-phosphate isomerase -
  AB8P72_RS00380 (AB8P72_00380) secG 84287..84595 (+) 309 WP_025652589.1 preprotein translocase subunit SecG -
  AB8P72_RS00395 (AB8P72_00395) rimP 85672..86172 (+) 501 WP_068407904.1 ribosome maturation factor RimP -

Sequence

Protein


Download         Length: 544 a.a.        Molecular weight: 60387.55 Da        Isoelectric Point: 5.2340

>NTDB_id=1033241 AB8P72_RS00370 WP_369957881.1 81121..82755(-) (pilB) [Psychrobacter sp. CLB018]
MKTAQTESQQQKMGLVPYLVDNKLANAYQVAQMLSQAFGDPLFDLDALSSDVIPKNLVDEKIVRKFNALPLFKRGQRLFV
ALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKKRIENLYADTMQSFDSFSDSDLNIGFNESEEDEGETKLSDGVDEAPV
VKFVNKMLVDAIRMGASDLHFEPYEKTYRVRFRVDGVMQKMASPPVQLAGKIAARLKVMSQMDISERRVPQDGRIKLKIS
KDKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQKEMFLEALHKPQGMLLITGPTGSGKTVSLYTGINILN
IGETNISTAEDPVEINLEGINQVNVNPKVGLTFANALKSFLRQDPDIVMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNS
APETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPINIPRQSLLELGFTDADLDNPDNVIYEPIGCNECREGYK
GRVGIYEVMKVSPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGILKVLQGVTSIQEMMRVTSE

Nucleotide


Download         Length: 1635 bp        

>NTDB_id=1033241 AB8P72_RS00370 WP_369957881.1 81121..82755(-) (pilB) [Psychrobacter sp. CLB018]
ATGAAAACCGCGCAAACAGAATCACAACAGCAGAAAATGGGCTTGGTTCCCTATTTGGTTGACAATAAGCTGGCCAATGC
TTATCAAGTAGCGCAGATGCTATCGCAAGCGTTTGGTGATCCTTTGTTTGATCTTGATGCCTTAAGCTCAGATGTCATTC
CAAAAAACTTGGTCGATGAAAAAATCGTACGTAAGTTCAACGCCCTACCACTGTTTAAACGCGGGCAACGCTTATTTGTG
GCATTGAGTGACCCGACCCGTATCGATGCCATCGACGCTATTGCCTTTAACTCGCGCCTGTCTATCGAGACTGTCGTCGT
TGAAGAAGATAAGCTCAAAAAGCGTATTGAGAATCTGTACGCTGATACCATGCAAAGTTTTGATAGCTTTTCTGACAGCG
ATTTAAATATTGGCTTTAATGAAAGCGAAGAAGACGAAGGCGAAACCAAACTTAGCGACGGCGTCGATGAAGCGCCCGTG
GTGAAGTTTGTTAATAAAATGTTGGTTGATGCCATTCGTATGGGCGCCTCGGACCTACATTTTGAACCTTATGAAAAAAC
CTATCGCGTGCGCTTTCGTGTCGATGGCGTCATGCAAAAAATGGCCAGTCCGCCGGTACAACTTGCGGGTAAAATTGCCG
CGCGTCTAAAAGTCATGTCGCAAATGGACATCTCTGAGCGCCGTGTACCGCAAGATGGTCGTATTAAGCTTAAAATCTCT
AAAGACAAAGCCATCGATTTCCGCGTAAACTCCCTACCTACCTTATTTGGCGAAAAGCTGGTTTTACGTATTTTGGATCC
ATCATCTGCCATGCTTGGTATTGAAGCATTAGGTTATGAGCCCGACCAAAAAGAGATGTTTTTAGAAGCCTTACATAAGC
CTCAAGGTATGCTGCTGATTACTGGTCCTACTGGTTCAGGTAAAACCGTCTCCCTTTATACCGGTATTAATATTTTAAAT
ATTGGCGAGACCAATATCTCAACCGCCGAAGACCCCGTTGAGATTAATCTTGAAGGTATCAATCAGGTCAACGTTAACCC
GAAAGTCGGTTTAACCTTTGCCAATGCGCTCAAATCATTCTTACGTCAAGATCCTGATATCGTCATGGTCGGTGAGATTC
GTGACCTTGAAACGGCTGAGATTGCGATTAAAGCAGCACAAACTGGACATATGGTGCTGTCTACCCTGCATACCAATTCT
GCCCCTGAAACCTTGACGCGTTTGCGCAATATGGGAGTTGCCTCATTTAACATTGCAACGTCAGTGAACTTGGTTATTGC
CCAGCGATTGGCAAGACGTTTATGCAAAAATTGCAAAAAACCCATCAATATACCGCGCCAAAGCTTGCTTGAGCTTGGTT
TTACCGATGCTGATTTAGACAACCCAGACAATGTCATTTATGAACCTATTGGCTGTAATGAATGCCGTGAAGGCTACAAA
GGCCGTGTCGGTATTTATGAAGTGATGAAAGTCAGCCCCGATATTTCGCGCATTATTATGGAAGATGGTAACGCCATAGA
TATCAAAGACGCTGCCCTTAAAAACGGCTTTAGAGATTTACGCCGCTCGGGTATTTTAAAGGTGCTGCAAGGGGTAACCA
GTATTCAAGAAATGATGCGCGTGACTTCTGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(166-433)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

69.343

100

0.699
  pilB Acinetobacter baylyi ADP1

66.055

100

0.662
  pilB Legionella pneumophila strain ERS1305867

54.004

98.713

0.533
  pilF Neisseria gonorrhoeae MS11

51.381

99.816

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

50.093

98.346

0.493
  pilB Vibrio cholerae strain A1552

50.095

97.243

0.487
  pilB Vibrio campbellii strain DS40M4

49.905

97.243

0.485
  pilB/pilB1 Synechocystis sp. PCC 6803

38.846

95.588

0.371
  pilF Thermus thermophilus HB27

36.697

100

0.368

Multiple sequence alignment