Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB8E34_RS15170 Genome accession   NZ_CP165964
Coordinates   3303027..3304733 (-) Length   568 a.a.
NCBI ID   WP_369958750.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain jeju3-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3298027..3309733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E34_RS15140 (AB8E34_15100) - 3298343..3298972 (-) 630 WP_318643449.1 DUF1780 domain-containing protein -
  AB8E34_RS15145 (AB8E34_15105) - 3299215..3299898 (+) 684 WP_369958745.1 energy-coupling factor ABC transporter permease -
  AB8E34_RS15150 (AB8E34_15110) yacG 3299958..3300158 (-) 201 WP_369958746.1 DNA gyrase inhibitor YacG -
  AB8E34_RS15155 (AB8E34_15115) coaE 3300155..3300763 (-) 609 WP_369958747.1 dephospho-CoA kinase -
  AB8E34_RS15160 (AB8E34_15120) pilD 3300924..3301796 (-) 873 WP_369958748.1 A24 family peptidase Machinery gene
  AB8E34_RS15165 (AB8E34_15125) pilC 3301800..3303023 (-) 1224 WP_369958749.1 type II secretion system F family protein Machinery gene
  AB8E34_RS15170 (AB8E34_15130) pilB 3303027..3304733 (-) 1707 WP_369958750.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8E34_RS15175 (AB8E34_15135) - 3304957..3305065 (+) 109 Protein_2965 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB8E34_RS15180 (AB8E34_15140) - 3305652..3305747 (+) 96 Protein_2966 transposase -
  AB8E34_RS15185 (AB8E34_15145) - 3305839..3306234 (+) 396 WP_369958751.1 nucleotidyltransferase domain-containing protein -
  AB8E34_RS15190 (AB8E34_15150) - 3306224..3306658 (+) 435 WP_369958752.1 DUF86 domain-containing protein -
  AB8E34_RS15195 (AB8E34_15155) nadC 3306727..3307575 (-) 849 WP_369958753.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62492.54 Da        Isoelectric Point: 5.0050

>NTDB_id=1032605 AB8E34_RS15170 WP_369958750.1 3303027..3304733(-) (pilB) [Pseudomonas benzenivorans strain jeju3-2]
MNESMSLTGLAKQMVLAELLDEKAAQQAQQQAQRNKTSLVTYLVQNKLVKSRALAELAADQFGVAFLDLASLDKEGQPRD
LVSEKLVRQHRALPLYRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIDRFFESATSGMEDLADVDLD
GLDVQAVDEDAQGNDAGGQEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRLRTDGMLHEVARPPIQLAPRISA
RLKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMTALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSSCKQEMDIPCEALLEEGF
KENQIGTFKIYAPVGCDNCKGGYKGRVGIYEVVKITPALQRIIMEEGNSIDIAAQMRKDGFNDLRTSALVKAMQGVTSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1032605 AB8E34_RS15170 WP_369958750.1 3303027..3304733(-) (pilB) [Pseudomonas benzenivorans strain jeju3-2]
ATGAACGAAAGCATGTCCCTCACCGGCCTGGCCAAACAGATGGTCCTCGCCGAGTTGCTCGACGAAAAAGCTGCCCAACA
AGCCCAACAACAGGCCCAGCGCAACAAGACCTCACTGGTCACCTATCTGGTACAAAACAAGCTGGTAAAGAGCCGGGCAC
TGGCCGAACTGGCTGCCGACCAATTTGGCGTGGCTTTTCTCGACCTAGCCTCACTCGATAAGGAAGGCCAACCCAGGGAT
CTGGTCAGCGAGAAGCTGGTTCGCCAGCACCGCGCCCTACCACTGTATCGCCGTGGCAATAAGCTGTTTATCGGCATATC
CGACCCCACCAACCACCAGGCCGTAACCGATATCCAGTTCAGCACCGGCCTGAACACCGAGGCCATCCTGGTCGAAGACG
ACAAGCTCGGCGACGCCATCGACAGATTCTTCGAGTCTGCCACCAGCGGCATGGAGGACCTGGCGGACGTCGACCTGGAT
GGGTTGGACGTTCAGGCCGTCGACGAGGACGCCCAGGGCAACGACGCTGGCGGCCAGGAAGCGGACGATGCTCCTGTGGT
GCGCTTCGTCAACAAGATGCTGCTCGATGCCATCAAGGGCGGCTCCTCCGACCTGCATTTCGAGCCCTACGAGAAGGTCT
ATCGGGTGCGTCTGCGCACCGACGGCATGCTCCACGAAGTCGCCCGCCCGCCCATCCAGCTGGCGCCGCGCATCTCCGCG
CGACTCAAGGTGATGGCCGCCCTGGACATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAAATATCCAA
GACCAAGGCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTCATGCGCATCCTCGACCCGA
GCAGCGCGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAAGAGCTGTACATGACAGCCCTCAAGCAGCCC
CAGGGCATGATCCTGGTGACCGGCCCTACCGGCTCGGGCAAGACCGTCTCCCTCTACACCGGCCTGAACATCCTCAACAC
CCCGGACGTGAATATCTCCACCGCAGAAGACCCGGTGGAGATCAACCTGGAGGGCATCAATCAGGTCAACGTCAACCCCA
AGCAGGGCATGGACTTCTCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGCGAGATTCGC
GACCTGGAGACTGCCGAGATCGCCATCAAGGCGGCCCAGACCGGCCATATGGTGATGTCCACCCTGCACACCAACAGCGC
CGCGGAAACTCTCACCCGCCTGCGCAACATGGGCGTGCCGGCGTTCAACATCGCCACCTCGGTCAACCTGATCATCGCCC
AGCGCCTGGCGCGCAAGCTGTGCAGCAGCTGCAAGCAGGAAATGGACATCCCCTGCGAGGCCCTGCTCGAGGAAGGTTTC
AAGGAAAACCAGATCGGCACCTTCAAGATCTACGCCCCGGTCGGTTGCGACAACTGCAAGGGCGGCTACAAGGGCCGCGT
CGGGATTTATGAAGTGGTTAAAATCACGCCAGCCCTGCAGCGGATTATCATGGAGGAAGGTAACTCCATCGATATCGCCG
CACAGATGCGCAAAGACGGCTTTAATGACCTACGCACCTCGGCACTGGTAAAGGCCATGCAAGGCGTCACCAGCCTCGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.649

100

0.599
  pilB Acinetobacter baylyi ADP1

58.156

99.296

0.577
  pilB Legionella pneumophila strain ERS1305867

56.316

100

0.565
  pilB Vibrio cholerae strain A1552

49.912

99.472

0.496
  pilF Neisseria gonorrhoeae MS11

49.027

99.472

0.488
  pilB Vibrio campbellii strain DS40M4

48.496

99.472

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

50

96.127

0.481
  pilF Thermus thermophilus HB27

37.478

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.658

92.782

0.368

Multiple sequence alignment