Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB6N02_RS00570 Genome accession   NZ_CP163510
Coordinates   112065..113174 (+) Length   369 a.a.
NCBI ID   WP_042882153.1    Uniprot ID   A0A2L0TPJ0
Organism   Aeromonas caviae strain 103     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 107065..118174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB6N02_RS00535 (AB6N02_00535) - 107100..107930 (+) 831 WP_223933205.1 CPBP family intramembrane glutamic endopeptidase -
  AB6N02_RS00540 (AB6N02_00540) - 108006..108425 (-) 420 WP_041210940.1 DUF4426 domain-containing protein -
  AB6N02_RS00545 (AB6N02_00545) yggU 108444..108743 (-) 300 WP_010673054.1 DUF167 family protein YggU -
  AB6N02_RS00550 (AB6N02_00550) - 108743..109294 (-) 552 WP_010673053.1 YggT family protein -
  AB6N02_RS00555 (AB6N02_00555) proC 109318..110142 (-) 825 WP_041210938.1 pyrroline-5-carboxylate reductase -
  AB6N02_RS00560 (AB6N02_00560) - 110266..110967 (-) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB6N02_RS00565 (AB6N02_00565) pilT 111007..112041 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  AB6N02_RS00570 (AB6N02_00570) pilU 112065..113174 (+) 1110 WP_042882153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB6N02_RS00575 (AB6N02_00575) yaaA 113207..113980 (+) 774 WP_103857984.1 peroxide stress protein YaaA -
  AB6N02_RS00580 (AB6N02_00580) srmB 114168..115388 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  AB6N02_RS00585 (AB6N02_00585) - 115516..116226 (+) 711 WP_202040292.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  AB6N02_RS00590 (AB6N02_00590) brnQ 116417..117724 (+) 1308 WP_223933202.1 branched-chain amino acid transport system II carrier protein -
  AB6N02_RS00595 (AB6N02_00595) - 117814..118041 (-) 228 WP_039039956.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40901.04 Da        Isoelectric Point: 6.3155

>NTDB_id=1030207 AB6N02_RS00570 WP_042882153.1 112065..113174(+) (pilU) [Aeromonas caviae strain 103]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1030207 AB6N02_RS00570 WP_042882153.1 112065..113174(+) (pilU) [Aeromonas caviae strain 103]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCTCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAACGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACAGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACTATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TPJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment