Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH33_RS02700 Genome accession   NZ_AP027940
Coordinates   575332..576441 (+) Length   369 a.a.
NCBI ID   WP_042026652.1    Uniprot ID   A0AAU6TAV3
Organism   Aeromonas enteropelogenes strain VA118     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570332..581441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH33_RS02670 (VAWG007_05050) - 571335..571754 (-) 420 WP_026457681.1 DUF4426 domain-containing protein -
  AACH33_RS02675 (VAWG007_05060) yggU 571791..572084 (-) 294 WP_042026655.1 DUF167 family protein YggU -
  AACH33_RS02680 (VAWG007_05070) - 572084..572635 (-) 552 WP_026457844.1 YggT family protein -
  AACH33_RS02685 (VAWG007_05080) proC 572647..573471 (-) 825 WP_338611755.1 pyrroline-5-carboxylate reductase -
  AACH33_RS02690 (VAWG007_05090) - 573528..574229 (-) 702 WP_338611756.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH33_RS02695 (VAWG007_05110) pilT 574269..575303 (+) 1035 WP_026457847.1 type IVa pilus ATPase TapT Machinery gene
  AACH33_RS02700 (VAWG007_05120) pilU 575332..576441 (+) 1110 WP_042026652.1 type IVa pilus ATPase TapU Machinery gene
  AACH33_RS02705 (VAWG007_05130) yaaA 576485..577258 (+) 774 WP_042026651.1 peroxide stress protein YaaA -
  AACH33_RS02710 (VAWG007_05140) - 577448..577873 (+) 426 WP_042026650.1 hypothetical protein -
  AACH33_RS02715 (VAWG007_05150) - 578228..578719 (-) 492 WP_338611757.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  AACH33_RS02720 (VAWG007_05160) arfB 578750..579166 (-) 417 WP_026456194.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  AACH33_RS02725 (VAWG007_05170) glnK 579368..579706 (+) 339 WP_026456195.1 P-II family nitrogen regulator -
  AACH33_RS02730 (VAWG007_05180) - 579845..580315 (-) 471 WP_026456196.1 MarR family transcriptional regulator -
  AACH33_RS02735 (VAWG007_05190) - 580475..581002 (+) 528 WP_042026648.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41169.16 Da        Isoelectric Point: 6.2710

>NTDB_id=103018 AACH33_RS02700 WP_042026652.1 575332..576441(+) (pilU) [Aeromonas enteropelogenes strain VA118]
MNLDQMLAELVSRKGSDLFVTVGSPPTLKVNGHLVALGNEPLDKKEALTLVRETLSSDHFERYIRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDDLSLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRGIVAQQLVPSIDGRRRFAAFEILLNTPLITDIIRKGEMHRLKEVMTKSSELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGHEQLGSGMLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=103018 AACH33_RS02700 WP_042026652.1 575332..576441(+) (pilU) [Aeromonas enteropelogenes strain VA118]
ATGAATCTGGATCAGATGCTGGCCGAGCTGGTCAGCCGCAAGGGCTCGGATCTGTTTGTGACGGTGGGCTCGCCTCCCAC
CCTCAAGGTCAATGGTCACCTGGTGGCGCTGGGCAACGAGCCCCTCGACAAGAAGGAGGCGCTGACCCTGGTCAGGGAGA
CCCTCAGCTCGGACCATTTTGAACGCTACATCCGAACCAAGGAGGCCAACTACGCCATCCATCGTGAGGAGCTGGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAAGAGCTGCCTGGCATGGTGCTGCGCCGCATCGAGACTCGCATTCCCACCTTCGA
TGATCTGAGCCTGCCTCCCATATTGCAGGACGTGGCGATGGCCAAGCGGGGACTGGTGTTGTTCGTCGGGGCCACGGGGG
CGGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGCGGATGGGCACATACTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCACCAACACGGCCGCAGCCTGGTGACCCAGCGCGAGGTGGGGATAGACACCGAATCGTTCGACGT
GGCGCTGAAGAGCTCTCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACTGGTCACCTCTGTCTGGCAACTCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAACACCGCCAGTTTCTGTTCGATCTCTCGTTCAACCTGCGTGGCATCGTTGCCCAGCA
ACTGGTGCCCAGTATCGACGGACGGCGACGTTTTGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCACCGGCTCAAGGAAGTGATGACCAAATCCTCAGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTGCCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCATGCCGACTCGGCCAATGACCTGCGACT
GCTGATCAAGCTTTCGGGTCATGAACAGCTGGGATCCGGCATGCTGGACAATGTGACGCTAGACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.689

95.122

0.558
  pilU Acinetobacter baylyi ADP1

56.091

95.664

0.537
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377

Multiple sequence alignment