Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB4P93_RS16180 Genome accession   NZ_CP162596
Coordinates   3396649..3398349 (+) Length   566 a.a.
NCBI ID   WP_210705183.1    Uniprot ID   -
Organism   Pseudomonas sp. B26140     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3391649..3403349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P93_RS16160 (AB4P93_16140) - 3391822..3394773 (+) 2952 WP_210705221.1 DUF748 domain-containing protein -
  AB4P93_RS16165 (AB4P93_16145) - 3394770..3395069 (+) 300 WP_210705186.1 DUF2845 domain-containing protein -
  AB4P93_RS16170 (AB4P93_16150) - 3395335..3395691 (-) 357 WP_150597113.1 BON domain-containing protein -
  AB4P93_RS16175 (AB4P93_16155) - 3395991..3396422 (-) 432 WP_210705185.1 pilin -
  AB4P93_RS16180 (AB4P93_16160) pilB 3396649..3398349 (+) 1701 WP_210705183.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB4P93_RS16185 (AB4P93_16165) pilC 3398352..3399566 (+) 1215 WP_395593182.1 type II secretion system F family protein Machinery gene
  AB4P93_RS16190 (AB4P93_16170) pilD 3399568..3400440 (+) 873 WP_210705179.1 A24 family peptidase Machinery gene
  AB4P93_RS16195 (AB4P93_16175) coaE 3400437..3401060 (+) 624 WP_210705178.1 dephospho-CoA kinase -
  AB4P93_RS16200 (AB4P93_16180) yacG 3401057..3401257 (+) 201 WP_210705177.1 DNA gyrase inhibitor YacG -
  AB4P93_RS16205 (AB4P93_16185) - 3401263..3401478 (-) 216 WP_042561116.1 hypothetical protein -
  AB4P93_RS16210 (AB4P93_16190) - 3401544..3402233 (-) 690 WP_210705176.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62655.55 Da        Isoelectric Point: 5.8308

>NTDB_id=1027337 AB4P93_RS16180 WP_210705183.1 3396649..3398349(+) (pilB) [Pseudomonas sp. B26140]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDTHGTGLEDMADVDLDG
LDVESIDDNKQDAIGGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREAAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKRAIEIPRETLIKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRMGIYEVVKNTADLQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLRKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1027337 AB4P93_RS16180 WP_210705183.1 3396649..3398349(+) (pilB) [Pseudomonas sp. B26140]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAAAAAGTGCCCAGCAGGC
CTGGCAACAGGCCCAGCGCAATCGGCTGTCGCTGGTCAGTTATCTGGTGCAGAACAAACTGGTAAAGAGCGCACAGGTCG
CCGAGATCGCCTCCGAGCACTTCGGCATGGCGTTCCTCGACCTTAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCCTATGGCGACGCGGCAACAAGCTGTTCGTGGGAATTTCCGA
CCCGAGCAATCATCAGGCAATCAACGACATCCAGTTCAGCACCGGGTTGAGCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAAAAATTCTTCGACACCCATGGCACCGGTCTCGAAGACATGGCCGACGTCGATCTCGACGGC
CTCGATGTCGAATCGATCGACGACAACAAGCAGGACGCCATCGGCGGACAAGACGCCGACGATGCGCCGGTGGTGCGTTT
CGTCCACAAGATGCTGCTCGATGCGATCAAAAGCGGCTCCTCCGACCTGCATTTCGAGCCTTACGAAAAAAACTACCGGG
TACGGGTGCGCACCGACGGCATTCTGCGCGAGGCGGCCAAGCCACCGATCCAACTGGCCGGGCGCATCGCCGCACGTTTG
AAAGTCATGGCCAGCCTCGACATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCCAAAAGCAA
GTCGATCGACTTCCGGGTCAATACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCCTCCAGCG
CGCAAATGGGCATTGACGCCCTCGGCTATGAGCCTGAGCAGAAAGACCTGTATTTGGCAGCCCTCAAGCAGCCACAAGGG
ATGATTCTGGTCACCGGCCCCACTGGCTCGGGCAAAACGGTGTCGCTGTACACCGGGCTGAATATTCTCAACACGGTCGA
CATCAACATATCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAACCCCAGACAGG
GGCTCGACTTTGCCCAAGCCTTGCGCTCGTTTCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTA
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGCCACCTCGTGCTTTCGACACTGCACACCAACAGCGCGGCGGA
AACCCTGACGCGACTGCATAACATGGGTATCCCGGGCTTCAACATCGCCACTTCGGTCAGCCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAGAGCCATCGAGATTCCGCGCGAAACCCTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTACGAGCCAGTCGGTTGCGATCACTGCAATGGCGGTTACAAGGGACGCATGGGGAT
TTATGAAGTGGTGAAGAACACAGCAGACCTGCAACGACTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACATCCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGTACCTCAGGCCTGCGCAAAGCCATGCAAGGCATTACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

56.915

99.647

0.567
  pilB Legionella pneumophila strain ERS1305867

55.221

99.823

0.551
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

49.296

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.548

93.816

0.371

Multiple sequence alignment