Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB4P91_RS10080 Genome accession   NZ_CP162590
Coordinates   2258529..2260229 (-) Length   566 a.a.
NCBI ID   WP_395604725.1    Uniprot ID   -
Organism   Pseudomonas sp. B21128     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2253529..2265229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P91_RS10050 (AB4P91_10040) - 2254642..2255331 (+) 690 WP_395604723.1 energy-coupling factor ABC transporter permease -
  AB4P91_RS10055 (AB4P91_10045) - 2255397..2255612 (+) 216 WP_395604724.1 hypothetical protein -
  AB4P91_RS10060 (AB4P91_10050) yacG 2255618..2255818 (-) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  AB4P91_RS10065 (AB4P91_10055) coaE 2255815..2256438 (-) 624 WP_150597110.1 dephospho-CoA kinase -
  AB4P91_RS10070 (AB4P91_10060) pilD 2256435..2257307 (-) 873 WP_150595329.1 A24 family peptidase Machinery gene
  AB4P91_RS10075 (AB4P91_10065) pilC 2257309..2258526 (-) 1218 WP_150595330.1 type II secretion system F family protein Machinery gene
  AB4P91_RS10080 (AB4P91_10070) pilB 2258529..2260229 (-) 1701 WP_395604725.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB4P91_RS10085 (AB4P91_10075) - 2260455..2260886 (+) 432 WP_150688181.1 pilin -
  AB4P91_RS10090 (AB4P91_10080) - 2261187..2261543 (+) 357 WP_150597113.1 BON domain-containing protein -
  AB4P91_RS10095 (AB4P91_10085) - 2261819..2262118 (-) 300 WP_150597114.1 DUF2845 domain-containing protein -
  AB4P91_RS10100 (AB4P91_10090) - 2262115..2265066 (-) 2952 WP_395604726.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62606.48 Da        Isoelectric Point: 5.8308

>NTDB_id=1027291 AB4P91_RS10080 WP_395604725.1 2258529..2260229(-) (pilB) [Pseudomonas sp. B21128]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAISDIQFSTGLSTEAILVEDDKLSDAIEKFFDTHGTGLEDMADVDLDG
LDVESIDDNKQDAIGGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKRAIEIPRETLIKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTADLQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLRKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1027291 AB4P91_RS10080 WP_395604725.1 2258529..2260229(-) (pilB) [Pseudomonas sp. B21128]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACAGAAAAAAGCGCCCAGCAGGC
CTGGCAACAGGCCCAGCGCAATCGGCTGTCGCTGGTCAGTTATCTGGTGCAGAACAAACTGGTTAAGAGCGCGCAGGTCG
CCGAGATCGCCTCCGAGCACTTCGGCATGGCGTTCCTCGACCTTAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCTTGTGGCGACGCGGCAACAAGCTGTTCGTGGGGATTTCCGA
CCCAAGCAATCATCAGGCAATCAGCGACATCCAGTTCAGCACCGGGTTGAGCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGACACCCACGGCACCGGTCTCGAAGACATGGCCGACGTCGATCTCGACGGC
CTCGATGTCGAATCGATCGACGACAACAAGCAGGACGCCATTGGCGGACAAGACGCCGACGATGCGCCGGTGGTGCGTTT
CGTCCACAAGATGCTGCTCGATGCGATCAAAAGCGGCTCCTCTGACCTGCATTTCGAGCCTTACGAAAAAAACTACCGGG
TACGGGTGCGCACCGACGGCATTCTGCGCGAGGTGGCCAAGCCACCGATCCAACTGGCCGGGCGCATCGCCGCACGGTTG
AAAGTCATGGCCAGCCTCGACATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCCAAAAGCAA
GTCGATCGACTTCCGGGTCAATACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTTATCCGGATTCTCGACCCCTCCAGCG
CGCAAATCGGCATCGACGCCCTCGGCTATGAGCCTGAGCAGAAAGACCTGTATTTGGCAGCCCTCAAGCAGCCACAAGGG
ATGATTCTGGTCACCGGCCCCACTGGCTCGGGCAAAACGGTGTCGCTGTACACCGGGCTGAATATTCTCAACACGGTCGA
CATCAACATATCCACCGCCGAAGACCCGGTGGAGATCAATATGGAGGGCATCAACCAGGTCAACGTCAACCCCAGACAGG
GGCTCGACTTTGCCCAGGCCTTGCGCTCGTTTCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGCCACCTCGTGCTTTCGACACTGCACACCAACAGCGCGGCGGA
AACCCTGACGCGACTGCATAACATGGGCATCCCCGGCTTCAACATCGCCACTTCGGTCAGCCTGATCATCGCCCAGCGGC
TGGCGCGCAAGTTGTGCAGCCACTGCAAGAGAGCCATCGAGATTCCGCGCGAAACCCTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTACGAGCCAGTCGGTTGCGATCACTGCAATGGCGGTTACAAGGGACGCGTGGGAAT
TTATGAAGTGGTGAAGAACACAGCAGACCTCCAACGACTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACATCCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGTACCTCAGGCCTGCGCAAGGCCATGCAAGGCATTACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.092

99.647

0.569
  pilB Legionella pneumophila strain ERS1305867

55.575

99.823

0.555
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

49.296

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.36

93.816

0.369

Multiple sequence alignment