Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB4P97_RS19075 Genome accession   NZ_CP162582
Coordinates   4076808..4078508 (+) Length   566 a.a.
NCBI ID   WP_395601472.1    Uniprot ID   -
Organism   Pseudomonas sp. A1230     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4071808..4083508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P97_RS19055 (AB4P97_19010) - 4071981..4074932 (+) 2952 WP_395602957.1 DUF748 domain-containing protein -
  AB4P97_RS19060 (AB4P97_19015) - 4074929..4075228 (+) 300 WP_115146562.1 DUF2845 domain-containing protein -
  AB4P97_RS19065 (AB4P97_19020) - 4075494..4075850 (-) 357 WP_042561109.1 BON domain-containing protein -
  AB4P97_RS19070 (AB4P97_19025) - 4076150..4076581 (-) 432 WP_042561110.1 pilin -
  AB4P97_RS19075 (AB4P97_19030) pilB 4076808..4078508 (+) 1701 WP_395601472.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB4P97_RS19080 (AB4P97_19035) pilC 4078511..4079728 (+) 1218 WP_042561112.1 type II secretion system F family protein Machinery gene
  AB4P97_RS19085 (AB4P97_19040) pilD 4079730..4080602 (+) 873 WP_150595329.1 A24 family peptidase Machinery gene
  AB4P97_RS19090 (AB4P97_19045) coaE 4080599..4081222 (+) 624 WP_395601473.1 dephospho-CoA kinase -
  AB4P97_RS19095 (AB4P97_19050) yacG 4081219..4081419 (+) 201 WP_042561115.1 DNA gyrase inhibitor YacG -
  AB4P97_RS19100 (AB4P97_19055) - 4081425..4081640 (-) 216 WP_042561116.1 hypothetical protein -
  AB4P97_RS19105 (AB4P97_19060) - 4081706..4082395 (-) 690 WP_042561117.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62621.52 Da        Isoelectric Point: 5.8308

>NTDB_id=1027183 AB4P97_RS19075 WP_395601472.1 4076808..4078508(+) (pilB) [Pseudomonas sp. A1230]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDNKQDAIGGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKRAIEIPREALIKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTADLQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLRKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1027183 AB4P97_RS19075 WP_395601472.1 4076808..4078508(+) (pilB) [Pseudomonas sp. A1230]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAAAAAGTGCCCAGCAGGC
CTGGCAACAGGCCCAGCGCAATCGGCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTAAAGAGCGCGCAGGTCG
CCGAGATCGCCTCCGAGCACTTCGGCATGGCGTTCCTCGACCTTAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCATGCCCTGCCCTTGTGGCGGCGCGGCAACAAGCTGTTCGTGGGAATTTCCGA
CCCGAGCAATCATCAGGCAATCAACGACATCCAGTTCAGCACCGGGTTGAGCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAAAAATTCTTCGACACTCACGCCAGCGGTCTCGAAGACATGGCCGACGTCGATCTCGACGGC
CTCGATGTCGAATCGATCGACGACAACAAGCAGGACGCCATCGGCGGACAAGACGCCGACGATGCGCCGGTGGTGCGTTT
CGTCCACAAGATGCTGCTCGATGCGATCAAAAGCGGCTCCTCCGACCTGCATTTCGAGCCTTACGAAAAAAACTACCGGG
TACGGGTGCGCACCGACGGCATTCTGCGCGAGGTTGCCAAGCCGCCGATCCAACTGGCCGGGCGCATCGCCGCACGTTTG
AAAGTCATGGCCAGCCTCGACATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCCAAAAGCAA
GTCGATCGACTTCCGGGTCAATACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATTCTCGACCCCTCCAGCG
CGCAAATGGGCATCGACGCCCTCGGCTATGAGCCTGAGCAGAAAGACCTGTATTTGGCAGCCCTCAAGCAGCCACAAGGG
ATGATTCTGGTCACCGGCCCCACTGGCTCGGGCAAAACGGTGTCGCTGTACACCGGGCTGAATATTCTCAACACGGTCGA
CATCAACATATCCACCGCCGAAGACCCGGTGGAGATCAATATGGAAGGCATCAACCAGGTCAACGTCAACCCCAGACAGG
GGCTCGACTTTGCCCAGGCCTTGCGCTCGTTTCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGCCACCTCGTGCTTTCGACACTGCACACCAACAGCGCCGCGGA
AACCCTGACGCGACTGCATAACATGGGCATCCCCGGCTTCAACATCGCCACTTCGGTCAGCCTGATCATCGCCCAGCGGC
TGGCGCGCAAGTTGTGCAGCCACTGCAAGAGAGCCATCGAGATTCCGCGCGAAGCCCTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTACGAGCCAGTCGGTTGCGATCACTGCAATGGCGGTTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAGAACACAGCAGACCTGCAACGACTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACATCCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGTACCTCAGGCCTGCGCAAGGCCATGCAAGGCATTACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

55.752

99.823

0.557
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

49.472

100

0.496
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.171

93.816

0.367

Multiple sequence alignment