Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB4P92_RS28590 Genome accession   NZ_CP162581
Coordinates   6197355..6198464 (+) Length   369 a.a.
NCBI ID   WP_395598557.1    Uniprot ID   -
Organism   Pseudomonas sp. A0308     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6192355..6203464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P92_RS28575 (AB4P92_28515) - 6193305..6194237 (+) 933 WP_261735754.1 sugar kinase -
  AB4P92_RS28580 (AB4P92_28520) - 6194363..6194638 (-) 276 WP_025112520.1 peptidylprolyl isomerase -
  AB4P92_RS28585 (AB4P92_28525) - 6194690..6197227 (-) 2538 WP_395598556.1 PAS domain-containing protein -
  AB4P92_RS28590 (AB4P92_28530) pilU 6197355..6198464 (+) 1110 WP_395598557.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB4P92_RS28595 (AB4P92_28535) - 6198595..6202362 (+) 3768 WP_395598558.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40987.95 Da        Isoelectric Point: 6.8375

>NTDB_id=1027169 AB4P92_RS28590 WP_395598557.1 6197355..6198464(+) (pilU) [Pseudomonas sp. A0308]
MEIDALLKQLASRHGLDLFLSTGAPPSARFEGVLTPLSEQSFKPGEVATVATSLMDAEQRREFDRDLEMNLAISRPGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLVLFVGATDSGKSTSLAALIDHRNRHSSGHIVTIED
PIEYIHRHQRSIINQREVGVDTRSFHSALKNTLRQAPDVVLIGEIRDRETMEHALSFAETGHLVLSTLHANNANQALDRI
INMFAEERRPQLLQALGNNLKACISQRLVRTVDGNRRAAVEVLLGTPTVADLVRRCEFGELKAMMEKSAAMGMQTFDSAL
FALVSTGAITEEEALRNADSVNNLKLRLKLIGESNIDRNSTAGEWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1027169 AB4P92_RS28590 WP_395598557.1 6197355..6198464(+) (pilU) [Pseudomonas sp. A0308]
ATGGAAATCGATGCACTGCTCAAACAACTGGCGAGTCGTCATGGTTTGGACCTGTTCCTCTCCACGGGCGCACCGCCCAG
TGCACGTTTTGAAGGCGTGCTTACCCCGTTGAGCGAGCAATCATTCAAACCTGGCGAGGTGGCAACGGTAGCCACCTCCC
TCATGGACGCCGAGCAGCGCAGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCGCGCCCCGGCATCGGTCGC
TTCCGGGTCAACATCTTTAAACAGCGCAACGATGTATCCATTGTGATCCGCAACGTCAAACTCGATATTCCGCGTTTCGA
GGATCTCAAACTGCCGCCAGTATTGCTCGAAACCGTGATGCTCAAGCAGGGGCTGGTACTGTTTGTCGGCGCCACCGATT
CGGGAAAATCAACCTCGCTGGCAGCGCTCATCGATCACCGCAACCGCCACAGCAGCGGACATATCGTCACCATCGAAGAC
CCGATCGAGTACATCCATCGCCATCAGCGGTCGATCATCAACCAGCGTGAAGTCGGGGTCGACACTCGAAGTTTTCACTC
CGCGCTTAAAAATACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGTGAAATTCGTGACCGTGAAACCATGGAGCACG
CGTTGTCATTTGCCGAGACAGGGCATCTGGTTTTGTCGACGTTGCACGCTAATAACGCCAATCAGGCGTTGGATCGAATT
ATCAATATGTTCGCAGAAGAGCGCCGGCCGCAGTTGCTACAGGCGCTTGGTAACAACTTGAAAGCATGTATTTCGCAGCG
TCTTGTGCGCACGGTTGATGGAAACCGCAGAGCTGCCGTAGAGGTGTTGCTGGGTACGCCGACCGTAGCCGATCTGGTCC
GACGTTGTGAGTTCGGGGAGCTCAAAGCCATGATGGAAAAATCCGCGGCTATGGGCATGCAGACATTTGACTCAGCCCTG
TTTGCGTTGGTATCGACGGGTGCAATTACTGAGGAAGAGGCGTTAAGGAATGCCGATTCGGTCAACAATCTGAAGTTGCG
CTTGAAATTGATCGGAGAGAGCAATATTGATCGAAATTCAACGGCGGGAGAGTGGGGTTTAATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.618

96.477

0.537
  pilU Acinetobacter baylyi ADP1

52.381

96.748

0.507
  pilU Vibrio cholerae strain A1552

50.857

94.851

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.058

92.683

0.371
  pilT Neisseria gonorrhoeae MS11

38.617

94.038

0.363
  pilT Neisseria meningitidis 8013

38.617

94.038

0.363

Multiple sequence alignment