Detailed information NTG1026982

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: ECTO148_RS25215
• Genome accession: NZ_LS992190
• Coordinates: 5058516..5060036 (+)
• Length: 506 a.a.
• NCBI ID: WP_000058957.1
• Uniprot ID: A0A0H3EP04
• Organism: Escherichia coli isolate Escherichia coli str. TO148
• Function: require for natural transformation (predicted from homology) Unclear

Genomic Context

Location: 5053516..5065036

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECTO148_RS25190 (ECTO148_04993)	ilvE	5055070..5055999 (-)	930	WP_000208520.1	branched-chain-amino-acid transaminase	-
ECTO148_RS25195 (ECTO148_04994)	ilvM	5056019..5056282 (-)	264	WP_000983255.1	acetolactate synthase 2 small subunit	-
ECTO148_RS25200 (ECTO148_04995)	ilvG	5056279..5057925 (-)	1647	WP_001012588.1	acetolactate synthase 2 catalytic subunit	-
ECTO148_RS25205	ilvX	5057928..5057978 (-)	51	WP_001387183.1	peptide IlvX	-
ECTO148_RS25210	ilvL	5058065..5058163 (-)	99	WP_001311244.1	ilv operon leader peptide	-
ECTO148_RS25215 (ECTO148_04996)	comM	5058516..5060036 (+)	1521	WP_000058957.1	YifB family Mg chelatase-like AAA ATPase	Machinery gene
ECTO148_RS25220 (ECTO148_04997)	maoP	5060061..5060399 (-)	339	WP_000841001.1	macrodomain Ori organization protein MaoP	-
ECTO148_RS25225 (ECTO148_04998)	hdfR	5060518..5061357 (+)	840	WP_000379257.1	HTH-type transcriptional regulator HdfR	-

Sequence

Protein

• Length: 506 a.a.
• Molecular weight: 55265.37 Da
• Isoelectric Point: 7.4104

>NTDB_id=1026982 ECTO148_RS25215 WP_000058957.1 5058516..5060036(+) (comM) [Escherichia coli isolate Escherichia coli str. TO148]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide

• Length: 1521 bp

>NTDB_id=1026982 ECTO148_RS25215 WP_000058957.1 5058516..5060036(+) (comM) [Escherichia coli isolate Escherichia coli str. TO148]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCAGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTTGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTGCCTATCGCTATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTTTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAACGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCTCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAACAGCACCACCGTTAAACAACGTGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAACGCCTG
GCTGGATAATCCGGAAATACGCCAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterProScan.

• Subunit ChlI of Mg-chelatase (ChlI) (21-142)

• Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C) (400-492)

• Magnesium chelatase, subunit ChlI (Mg_chelatase) (189-386)

3D structure

• AlphaFold DB: A0A0H3EP04

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Haemophilus influenzae Rd KW20	58.317	100	0.589
comM	Glaesserella parasuis strain SC1401	58.58	100	0.587
comM	Vibrio cholerae strain A1552	58.765	99.209	0.583
comM	Vibrio cholerae O1 biovar El Tor strain E7946	58.765	99.209	0.583
comM	Vibrio campbellii strain DS40M4	57.769	99.209	0.573
comM	Acinetobacter baylyi ADP1	48.996	98.419	0.482
comM	Legionella pneumophila str. Paris	47.791	98.419	0.47
comM	Legionella pneumophila strain ERS1305867	47.791	98.419	0.47
RA0C_RS07335	Riemerella anatipestifer ATCC 11845 = DSM 15868	44.071	100	0.441