Detailed information NTG1026836

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB3464_RS02685
• Genome accession: NZ_CP162519
• Coordinates: 712579..713745 (-)
• Length: 388 a.a.
• NCBI ID: WP_010451351.1
• Uniprot ID: A0A1H1XWA0
• Organism: Pseudomonas asplenii strain UPB 526
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 707579..718745

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3464_RS02670 (AB3464_02635)	-	708395..709873 (+)	1479	WP_397458872.1	mannitol dehydrogenase family protein	-
AB3464_RS02675 (AB3464_02640)	xylB	710053..711543 (+)	1491	WP_397458873.1	xylulokinase	-
AB3464_RS02680 (AB3464_02645)	-	711550..712488 (+)	939	WP_010451352.1	carbohydrate kinase	-
AB3464_RS02685 (AB3464_02650)	pilU	712579..713745 (-)	1167	WP_010451351.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB3464_RS02690 (AB3464_02655)	-	713881..716415 (+)	2535	WP_397458874.1	PAS domain-containing protein	-
AB3464_RS02695 (AB3464_02660)	-	716474..716749 (+)	276	WP_007932676.1	peptidylprolyl isomerase	-
AB3464_RS02700 (AB3464_02665)	-	716737..717669 (-)	933	WP_397458875.1	sugar kinase	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 42995.19 Da
• Isoelectric Point: 6.1925

>NTDB_id=1026836 AB3464_RS02685 WP_010451351.1 712579..713745(-) (pilU) [Pseudomonas asplenii strain UPB 526]
MEIHALLKLLAAQDGSDLYLSTGAPPCAKFNGVLKPLGTDALKAGEVALIADGIMDAEQRQQFERELEMNLALSLPGVGR
FRVNIFKQRNEVSIVARNIKLEIPRFEDLKLPPILLDTIMEKRGLVLFVGATGSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLQAFVSQRLVRTTDGKRRAAVEVMLGTPTVRDFIKRNEFSELKGIMEKSEVSGMLTFDTAL
FNLVVEGAIDEDEALKNADSVNNLRLRLKLHQDGAAIQQKTPPAPPVAAQELDTKDWGLVDDIDTPAQ

Nucleotide

• Length: 1167 bp

>NTDB_id=1026836 AB3464_RS02685 WP_010451351.1 712579..713745(-) (pilU) [Pseudomonas asplenii strain UPB 526]
ATGGAAATCCACGCCTTATTGAAACTCCTTGCCGCCCAGGACGGCTCGGATCTTTACCTGTCGACCGGCGCACCGCCCTG
TGCCAAGTTCAATGGCGTGCTCAAGCCGCTGGGCACCGACGCACTCAAGGCCGGCGAGGTGGCGCTGATTGCCGACGGTA
TCATGGACGCCGAACAGCGCCAGCAGTTCGAACGCGAGCTGGAAATGAACCTGGCGTTGTCATTGCCGGGCGTCGGGCGT
TTTCGGGTGAATATTTTCAAGCAACGCAACGAAGTCTCCATCGTTGCCCGCAATATCAAGCTGGAGATCCCTCGTTTCGA
AGACCTCAAGCTGCCGCCGATCCTGCTCGACACCATCATGGAAAAGCGCGGCCTGGTGCTCTTCGTCGGTGCTACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGGCACAGCAGTGGCCATATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAAGTGGGGGTCGACACCCGCAGTTTCCATGC
GGCGCTGAAAAATACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTTTTCCCGGAAGACCGCCGCCCGCAGTTGCTCAACGACCTGGGCAACAACCTGCAGGCCTTCGTCTCCCAACG
GCTGGTCCGTACCACCGATGGCAAGCGCCGGGCGGCGGTCGAGGTGATGCTGGGGACGCCGACCGTTCGCGACTTCATCA
AGCGCAACGAGTTCTCTGAACTCAAGGGCATTATGGAGAAGTCCGAAGTCAGCGGCATGCTGACGTTCGATACGGCGCTG
TTCAACCTGGTGGTGGAGGGCGCGATCGACGAGGACGAGGCACTGAAGAATGCCGACTCGGTGAACAACCTGCGTCTGCG
GCTGAAATTGCACCAGGACGGCGCGGCGATCCAGCAGAAAACCCCACCGGCCCCTCCCGTGGCGGCACAGGAGCTGGATA
CCAAGGATTGGGGCCTGGTCGACGACATCGATACACCGGCTCAGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-271)

3D structure

• AlphaFold DB: A0A1H1XWA0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.98	90.464	0.515
pilU	Acinetobacter baylyi ADP1	52.809	91.753	0.485
pilU	Vibrio cholerae strain A1552	50.571	90.206	0.456
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40	95.361	0.381
pilT	Pseudomonas stutzeri DSM 10701	41.159	88.918	0.366
pilT	Pseudomonas aeruginosa PAK	40.58	88.918	0.361