Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB3G31_RS04350 Genome accession   NZ_CP162006
Coordinates   895864..897597 (-) Length   577 a.a.
NCBI ID   WP_367848983.1    Uniprot ID   -
Organism   Rhodoferax sp. WC2427     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 890864..902597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3G31_RS04320 (AB3G31_04320) - 891598..892065 (+) 468 WP_367848977.1 NUDIX domain-containing protein -
  AB3G31_RS04325 (AB3G31_04325) - 892117..892317 (-) 201 WP_367848978.1 DNA gyrase inhibitor YacG -
  AB3G31_RS04330 (AB3G31_04330) zapD 892375..893130 (-) 756 WP_367848979.1 cell division protein ZapD -
  AB3G31_RS04335 (AB3G31_04335) coaE 893152..893694 (-) 543 WP_367848980.1 dephospho-CoA kinase -
  AB3G31_RS04340 (AB3G31_04340) pilD 893765..894604 (-) 840 WP_367848981.1 A24 family peptidase Machinery gene
  AB3G31_RS04345 (AB3G31_04345) pilC 894613..895830 (-) 1218 WP_367848982.1 type II secretion system F family protein Machinery gene
  AB3G31_RS04350 (AB3G31_04350) pilB 895864..897597 (-) 1734 WP_367848983.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB3G31_RS04355 (AB3G31_04355) - 897638..898630 (-) 993 WP_367848984.1 RluA family pseudouridine synthase -
  AB3G31_RS04360 (AB3G31_04360) - 899225..902455 (+) 3231 WP_367848985.1 Rne/Rng family ribonuclease -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62961.59 Da        Isoelectric Point: 6.1508

>NTDB_id=1023449 AB3G31_RS04350 WP_367848983.1 895864..897597(-) (pilB) [Rhodoferax sp. WC2427]
MAAVEPFTKESSSVALPGLGRVLISAGKLGQRAAEDIYRKAQSNRASFIAELMSSGAVSASDLAHTMSTAFAAPLLDIDA
VDRHRLPKDLLDAKICQAYRVVVLSKRNNRLIVATADPSDQEAAEKIKFATQLGVDWIIAEYDKLSRMVDANAVTTGEAM
DNIIGGDFDFDDAIMGTPVEAAEINMTEVEDAPVVKFLHKMLLDAYGMRASDLHFEPYEHNYRVRFRIDGELREIASPPV
AIKDKLASRIKVISRLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
VMQAIGRPYGMVLVTGPTGSGKTVSLYTFLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGMTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKALIDIPQ
KTLLESGYKPEELDGSWKPYGPVGCNACNNGYKGRVGIYQVMPISEEIQRIILRDGSALDIAEQAKIEGVRTLRQAGLHK
VKLGLTSLEEVLAVSNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1023449 AB3G31_RS04350 WP_367848983.1 895864..897597(-) (pilB) [Rhodoferax sp. WC2427]
ATGGCCGCTGTCGAACCCTTCACCAAAGAATCATCTTCTGTTGCGCTTCCCGGCTTGGGAAGGGTTCTGATTTCTGCAGG
CAAACTCGGCCAACGCGCGGCCGAAGACATCTACCGCAAAGCCCAGTCCAACCGCGCCAGCTTCATCGCTGAACTGATGT
CCTCCGGGGCGGTGTCGGCATCGGACCTGGCGCACACCATGTCCACCGCGTTTGCCGCGCCTCTGCTGGACATCGACGCG
GTCGACCGCCACCGCCTGCCCAAAGACCTGCTGGACGCCAAAATCTGCCAAGCCTACCGGGTGGTGGTGCTGAGCAAACG
CAACAACCGCCTGATCGTTGCCACCGCCGACCCGTCGGACCAGGAAGCCGCCGAGAAAATCAAGTTTGCGACCCAGCTGG
GGGTGGACTGGATCATCGCCGAATACGACAAGCTGTCGCGCATGGTGGATGCCAACGCCGTCACCACCGGTGAGGCGATG
GACAACATCATCGGCGGCGACTTCGACTTTGACGACGCGATCATGGGCACGCCGGTCGAGGCCGCCGAAATCAACATGAC
CGAGGTGGAAGATGCCCCGGTCGTCAAGTTTTTGCACAAGATGCTGCTCGATGCCTATGGCATGCGCGCATCCGACCTGC
ACTTTGAGCCCTACGAGCACAACTACCGCGTGCGCTTTCGTATCGACGGTGAACTGCGCGAAATCGCCTCGCCGCCGGTG
GCCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAAAAACGGGTGCCGCAAGACGG
CAAGATGAAGCTCAAAATCGGCCCGGACCGGGTGATCGACTTCCGCGTCAGCACCTTGCCCACCCTGTTCGGCGAAAAGA
TCGTGATCCGGATTCTGGACCCGAGCAGCGCCAAACTCGGCATCGATGCGCTGGGCTACGAGCCCGAAGAAAAAGAGCGG
GTGATGCAGGCCATTGGCCGCCCCTACGGCATGGTGCTGGTCACCGGGCCTACGGGATCGGGCAAAACGGTGTCGCTGTA
CACCTTCTTGAACCTGCTGAACAAGCCCGGCGTGAACATCGCCACGGCCGAAGACCCGTCCGAAATCAACCTGCCGGGCG
TGAACCAGGTCAATGTGAACGAAAAAGCCGGCATGACCTTTGCCGTGGCCCTGAAGTCGTTTCTGCGCCAAGACCCGGAC
ATCATCATGGTGGGCGAAATCCGCGATCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAAACCGGCCATTTGGTGTT
GTCCACGCTGCACACCAACGACGCACCCACCACCTTGACGCGCATGCGCAATATGGGCATTGCGCCGTTCAATATCGCCT
CCAGCGTGATCCTGATCACCGCCCAGCGGCTGGCACGCCGCCTGTGCCCCGCGTGCAAGGCGCTCATCGACATCCCGCAA
AAGACCTTGCTGGAGTCCGGCTACAAGCCCGAAGAGCTGGACGGCTCCTGGAAGCCCTACGGCCCGGTGGGCTGCAACGC
CTGCAACAACGGCTACAAAGGCCGGGTGGGTATCTACCAGGTGATGCCGATTTCCGAAGAAATCCAGCGCATCATCCTGC
GCGATGGCAGTGCGCTGGATATTGCAGAACAGGCCAAAATCGAAGGTGTGCGGACCCTGCGCCAGGCCGGGTTGCACAAG
GTGAAGCTGGGCTTGACCTCGCTGGAAGAAGTGCTCGCTGTGAGCAATGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.832

97.227

0.523
  pilB Acinetobacter baumannii D1279779

52.734

98.267

0.518
  pilF Neisseria gonorrhoeae MS11

51.95

97.747

0.508
  pilB Legionella pneumophila strain ERS1305867

49.733

97.227

0.484
  pilB Vibrio campbellii strain DS40M4

44.149

97.747

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

44.207

97.227

0.43
  pilB Vibrio cholerae strain A1552

46.667

90.988

0.425
  pilF Thermus thermophilus HB27

37.368

98.787

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.245

91.854

0.36

Multiple sequence alignment