Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABZN20_RS00450 Genome accession   NZ_CP160399
Coordinates   94202..95917 (+) Length   571 a.a.
NCBI ID   WP_367026430.1    Uniprot ID   -
Organism   Methylococcus sp. ANG     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 89202..100917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZN20_RS00435 (ABZN20_00435) - 89374..90867 (+) 1494 WP_218806192.1 leucyl aminopeptidase -
  ABZN20_RS00440 (ABZN20_00440) - 90864..91295 (+) 432 WP_218806193.1 DNA polymerase III subunit chi -
  ABZN20_RS00445 (ABZN20_00445) - 91390..94152 (+) 2763 WP_367026429.1 valine--tRNA ligase -
  ABZN20_RS00450 (ABZN20_00450) pilB 94202..95917 (+) 1716 WP_367026430.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABZN20_RS00455 (ABZN20_00455) pilC 95922..97136 (+) 1215 WP_218806196.1 type II secretion system F family protein Machinery gene
  ABZN20_RS00460 (ABZN20_00460) pilD 97147..98025 (+) 879 WP_218806197.1 A24 family peptidase Machinery gene
  ABZN20_RS00465 (ABZN20_00465) coaE 98033..98629 (+) 597 WP_367026431.1 dephospho-CoA kinase -
  ABZN20_RS00470 (ABZN20_00470) zapD 98761..99498 (+) 738 WP_367026432.1 cell division protein ZapD -
  ABZN20_RS00475 (ABZN20_00475) yacG 99509..99724 (+) 216 WP_218806200.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63154.89 Da        Isoelectric Point: 5.8943

>NTDB_id=1020880 ABZN20_RS00450 WP_367026430.1 94202..95917(+) (pilB) [Methylococcus sp. ANG]
MAAVSGVQQLSGLAKSLVKAGMLSEADAQAHFEDARRKKTPFVSYLVSNKILDGLQIAQVAHQEFGLPLLDLDAVVFDIP
PTQWVSEKLIRRHHILPLLRRGNHLFVAVSDPTNFLGLDEITFQTRLVTDCILVEENKLSRCIEAVLDAADTSLKQLLEE
DLDSLQITAGDDEGSHAEPEISGIDDAPIVRFVNRLLLDAVRKGASDIHIEPYEKFLRIRFRHDGILHEVANPPAALGIK
ICARIKVMSRMDIAEHRIPQDGRIKMTLSRSRAIDFRVNTCPTLFGEKIVLRILDPGSAQIGVEMLGFEPEQRDYFLRAL
EKPYGMILVTGPTGSGKTVTLYTGLNLLNRVEANISTVEDPVEITVPGINQVNVNYKTGLTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVLLIMAQRLARRLCPHCKREEKLPPEVLLA
SGFREEDIGNFTLYGPGGCEQCVKGYKGRVGIYQVMPISEEINRIILEGGHVMALTEQAHAEGIADLRESGLKKVRAGIT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1020880 ABZN20_RS00450 WP_367026430.1 94202..95917(+) (pilB) [Methylococcus sp. ANG]
ATGGCCGCGGTTTCCGGCGTCCAGCAACTGAGCGGCCTGGCGAAAAGCCTGGTCAAGGCCGGGATGCTTTCCGAGGCGGA
TGCCCAGGCACATTTCGAGGATGCGCGCCGGAAGAAGACCCCTTTCGTCAGCTATCTCGTCTCCAACAAGATCCTCGACG
GTCTCCAGATCGCCCAGGTCGCCCACCAGGAGTTCGGGCTGCCGCTGCTCGATCTGGACGCGGTCGTCTTCGATATTCCC
CCGACCCAGTGGGTCAGCGAGAAGCTGATACGGCGCCATCACATCCTGCCCTTGCTGCGGCGGGGCAACCATCTGTTCGT
CGCGGTCTCGGATCCGACCAATTTTCTGGGGCTGGACGAAATCACCTTCCAGACCCGTCTCGTCACCGATTGCATCCTGG
TCGAGGAGAACAAACTCTCCCGCTGCATCGAAGCGGTCCTGGACGCGGCCGACACCAGCCTCAAGCAACTGCTCGAGGAG
GACCTGGACAGTCTCCAGATCACCGCCGGGGACGACGAAGGCTCGCATGCCGAACCGGAAATTTCCGGCATCGACGATGC
GCCGATCGTGCGTTTCGTCAACCGGCTCCTGCTGGACGCGGTGCGAAAAGGGGCGTCGGACATCCATATCGAGCCCTACG
AAAAATTCCTGCGCATCCGGTTCCGGCACGACGGCATCCTGCACGAGGTGGCGAACCCGCCGGCGGCGCTGGGAATCAAG
ATCTGCGCCCGCATCAAGGTGATGTCGCGCATGGACATCGCGGAACACCGGATACCCCAGGACGGCCGCATCAAGATGAC
GTTGTCGCGTTCCCGCGCCATCGATTTCCGTGTCAATACCTGCCCGACGCTGTTCGGGGAAAAGATCGTTCTGCGTATCC
TCGATCCGGGTTCAGCCCAGATCGGCGTCGAAATGCTGGGATTCGAACCGGAGCAGCGGGACTATTTCCTGCGTGCCCTG
GAAAAACCCTACGGCATGATTCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCGTCACCCTCTACACCGGCCTGAACCT
GCTCAATCGCGTCGAAGCCAACATCTCCACGGTGGAGGACCCGGTCGAAATCACGGTCCCGGGGATCAATCAGGTCAACG
TCAACTACAAGACCGGCCTCACGTTCGCCGAGGCGCTGCGCGCATTTCTGCGCCAGGACCCCGACATCATCATGGTGGGC
GAGATCCGCGACCTGGAGACCGCCGAGATCGCCGTCAAGGCGGCCCAGACCGGCCATCTGGTGCTTTCGACCCTGCATAC
CAACGATGCCCCTCAGACCTTGAACCGTCTGATGCAGATGGGCATCCCGGCGTTCAACATCGCCTCCTCCGTCCTCCTGA
TCATGGCGCAGCGGCTGGCCCGCAGGCTTTGTCCGCACTGCAAGCGGGAGGAGAAGCTGCCGCCCGAGGTTCTGCTCGCG
TCCGGATTCCGGGAGGAAGATATCGGCAATTTCACGCTTTACGGACCGGGGGGCTGCGAGCAGTGCGTGAAAGGCTACAA
GGGGCGGGTCGGCATCTACCAGGTCATGCCGATTTCGGAGGAAATCAACCGCATCATCCTGGAAGGCGGGCACGTGATGG
CCCTGACCGAGCAGGCGCATGCGGAAGGCATCGCCGATCTGAGGGAGTCCGGCTTGAAGAAAGTGAGGGCGGGCATCACC
AGCCTCGAGGAAATCGACCGCGTGACGAGGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56

100

0.564
  pilB Acinetobacter baylyi ADP1

52.941

100

0.536
  pilB Acinetobacter baumannii D1279779

51.832

100

0.52
  pilB Vibrio cholerae strain A1552

48.85

98.949

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

47.865

98.424

0.471
  pilF Neisseria gonorrhoeae MS11

47.34

98.774

0.468
  pilB Vibrio campbellii strain DS40M4

46.536

98.599

0.459
  pilF Thermus thermophilus HB27

38.313

99.65

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

38.779

97.548

0.378

Multiple sequence alignment