Detailed information NTG1020133

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB0R86_RS12465
• Genome accession: NZ_CP160227
• Coordinates: 2560455..2561069 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JJ947
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2555455..2566069

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0R86_RS12435 (AB0R86_12435)	rpsT	2555758..2556024 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB0R86_RS12440 (AB0R86_12440)	holA	2556041..2557082 (-)	1042	Protein_2403	DNA polymerase III subunit delta	-
AB0R86_RS12445 (AB0R86_12445)	-	2557140..2557273 (-)	134	Protein_2404	hypothetical protein	-
AB0R86_RS12450 (AB0R86_12450)	-	2557314..2557448 (+)	135	WP_003152870.1	YqzM family protein	-
AB0R86_RS12455 (AB0R86_12455)	comEC	2557467..2559818 (-)	2352	WP_007408265.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB0R86_RS12460 (AB0R86_12460)	-	2559819..2560388 (-)	570	WP_003152868.1	ComE operon protein 2	-
AB0R86_RS12465 (AB0R86_12465)	comEA	2560455..2561069 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB0R86_RS12470 (AB0R86_12470)	comER	2561128..2561949 (+)	822	WP_032866690.1	late competence protein ComER	-
AB0R86_RS12475 (AB0R86_12475)	-	2562018..2562755 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
AB0R86_RS12480 (AB0R86_12480)	rsfS	2562752..2563108 (-)	357	WP_007408260.1	ribosome silencing factor	-
AB0R86_RS12485 (AB0R86_12485)	yqeK	2563125..2563685 (-)	561	WP_335395283.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB0R86_RS12490 (AB0R86_12490)	-	2563675..2564244 (-)	570	WP_042635364.1	nicotinate-nucleotide adenylyltransferase	-
AB0R86_RS12495 (AB0R86_12495)	yhbY	2564255..2564545 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
AB0R86_RS12500 (AB0R86_12500)	aroE	2564539..2565381 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=1020133 AB0R86_RS12465 WP_032866693.1 2560455..2561069(-) (comEA) [Bacillus velezensis strain JJ947]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1020133 AB0R86_RS12465 WP_032866693.1 2560455..2561069(-) (comEA) [Bacillus velezensis strain JJ947]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363