Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB0R82_RS12365 Genome accession   NZ_CP160220
Coordinates   2418709..2419779 (-) Length   356 a.a.
NCBI ID   WP_129134318.1    Uniprot ID   -
Organism   Bacillus subtilis strain JM553     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2413709..2424779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R82_RS12320 (AB0R82_12320) tapA 2414188..2414949 (-) 762 WP_198878634.1 amyloid fiber anchoring/assembly protein TapA -
  AB0R82_RS12325 (AB0R82_12325) yqzG 2415221..2415547 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  AB0R82_RS12330 (AB0R82_12330) spoIITA 2415589..2415768 (-) 180 WP_072175549.1 YqzE family protein -
  AB0R82_RS12335 (AB0R82_12335) comGG 2415839..2416213 (-) 375 WP_133953114.1 ComG operon protein ComGG Machinery gene
  AB0R82_RS12340 (AB0R82_12340) comGF 2416214..2416597 (-) 384 WP_032726158.1 ComG operon protein ComGF Machinery gene
  AB0R82_RS12345 (AB0R82_12345) comGE 2416623..2416970 (-) 348 WP_080529537.1 ComG operon protein 5 Machinery gene
  AB0R82_RS12350 (AB0R82_12350) comGD 2416954..2417385 (-) 432 WP_080529538.1 comG operon protein ComGD Machinery gene
  AB0R82_RS12355 (AB0R82_12355) comGC 2417375..2417671 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  AB0R82_RS12360 (AB0R82_12360) comGB 2417685..2418722 (-) 1038 WP_044052501.1 comG operon protein ComGB Machinery gene
  AB0R82_RS12365 (AB0R82_12365) comGA 2418709..2419779 (-) 1071 WP_129134318.1 competence protein ComGA Machinery gene
  AB0R82_RS12370 (AB0R82_12370) - 2419991..2420188 (-) 198 WP_129134319.1 hypothetical protein -
  AB0R82_RS12375 (AB0R82_12375) corA 2420190..2421143 (-) 954 WP_198878633.1 magnesium transporter CorA -
  AB0R82_RS12380 (AB0R82_12380) yqhB 2421286..2422611 (+) 1326 WP_198878632.1 hemolysin family protein -
  AB0R82_RS12385 (AB0R82_12385) rsbRD 2422668..2423504 (-) 837 WP_015714260.1 RsbT co-antagonist protein RsbRD -
  AB0R82_RS12395 (AB0R82_12395) mgsR 2423728..2424108 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  AB0R82_RS12400 (AB0R82_12400) yqgY 2424340..2424585 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40442.58 Da        Isoelectric Point: 9.1529

>NTDB_id=1019703 AB0R82_RS12365 WP_129134318.1 2418709..2419779(-) (comGA) [Bacillus subtilis strain JM553]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEESVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1019703 AB0R82_RS12365 WP_129134318.1 2418709..2419779(-) (comGA) [Bacillus subtilis strain JM553]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGAGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997
  pilF Thermus thermophilus HB27

36.827

99.157

0.365

Multiple sequence alignment