Detailed information NTG1019477

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB0R90_RS13280
• Genome accession: NZ_CP160217
• Coordinates: 2688027..2688641 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain AP52
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2683027..2693641

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0R90_RS13250 (AB0R90_13250)	rpsT	2683330..2683596 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB0R90_RS13255 (AB0R90_13255)	holA	2683613..2684654 (-)	1042	Protein_2566	DNA polymerase III subunit delta	-
AB0R90_RS13260 (AB0R90_13260)	-	2684712..2684845 (-)	134	Protein_2567	hypothetical protein	-
AB0R90_RS13265 (AB0R90_13265)	-	2684886..2685020 (+)	135	WP_003152870.1	YqzM family protein	-
AB0R90_RS13270 (AB0R90_13270)	comEC	2685039..2687390 (-)	2352	WP_076424994.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB0R90_RS13275 (AB0R90_13275)	-	2687391..2687960 (-)	570	WP_003152868.1	ComE operon protein 2	-
AB0R90_RS13280 (AB0R90_13280)	comEA	2688027..2688641 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB0R90_RS13285 (AB0R90_13285)	comER	2688700..2689521 (+)	822	WP_015240246.1	late competence protein ComER	-
AB0R90_RS13290 (AB0R90_13290)	-	2689590..2690327 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
AB0R90_RS13295 (AB0R90_13295)	rsfS	2690324..2690680 (-)	357	WP_007408260.1	ribosome silencing factor	-
AB0R90_RS13300 (AB0R90_13300)	yqeK	2690698..2691258 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB0R90_RS13305 (AB0R90_13305)	-	2691248..2691817 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
AB0R90_RS13310 (AB0R90_13310)	yhbY	2691828..2692118 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
AB0R90_RS13315 (AB0R90_13315)	aroE	2692112..2692954 (-)	843	WP_059368084.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=1019477 AB0R90_RS13280 WP_032866693.1 2688027..2688641(-) (comEA) [Bacillus velezensis strain AP52]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1019477 AB0R90_RS13280 WP_032866693.1 2688027..2688641(-) (comEA) [Bacillus velezensis strain AP52]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTATAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363