Detailed information NTG1019176

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB0R80_RS13305
• Genome accession: NZ_CP160213
• Coordinates: 2716725..2717339 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JM199
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2711725..2722339

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0R80_RS13275 (AB0R80_13275)	rpsT	2712029..2712295 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB0R80_RS13280 (AB0R80_13280)	holA	2712312..2713353 (-)	1042	Protein_2571	DNA polymerase III subunit delta	-
AB0R80_RS13285 (AB0R80_13285)	-	2713393..2713544 (-)	152	Protein_2572	hypothetical protein	-
AB0R80_RS13290 (AB0R80_13290)	-	2713585..2713719 (+)	135	WP_003152870.1	YqzM family protein	-
AB0R80_RS13295 (AB0R80_13295)	comEC	2713738..2716089 (-)	2352	WP_254003296.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB0R80_RS13300 (AB0R80_13300)	-	2716090..2716659 (-)	570	WP_003152868.1	ComE operon protein 2	-
AB0R80_RS13305 (AB0R80_13305)	comEA	2716725..2717339 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB0R80_RS13310 (AB0R80_13310)	comER	2717398..2718219 (+)	822	WP_254003297.1	late competence protein ComER	-
AB0R80_RS13315 (AB0R80_13315)	-	2718288..2719025 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
AB0R80_RS13320 (AB0R80_13320)	rsfS	2719022..2719378 (-)	357	WP_007408260.1	ribosome silencing factor	-
AB0R80_RS13325 (AB0R80_13325)	yqeK	2719396..2719956 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB0R80_RS13330 (AB0R80_13330)	-	2719946..2720515 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
AB0R80_RS13335 (AB0R80_13335)	yhbY	2720526..2720816 (-)	291	WP_061860725.1	ribosome assembly RNA-binding protein YhbY	-
AB0R80_RS13340 (AB0R80_13340)	aroE	2720810..2721652 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=1019176 AB0R80_RS13305 WP_032866693.1 2716725..2717339(-) (comEA) [Bacillus velezensis strain JM199]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1019176 AB0R80_RS13305 WP_032866693.1 2716725..2717339(-) (comEA) [Bacillus velezensis strain JM199]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363