Detailed information NTG1019100

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB0R70_RS12380
• Genome accession: NZ_CP160212
• Coordinates: 2552152..2552766 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JM204
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2547152..2557766

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0R70_RS12350 (AB0R70_12350)	rpsT	2547455..2547721 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB0R70_RS12355 (AB0R70_12355)	holA	2547738..2548779 (-)	1042	Protein_2386	DNA polymerase III subunit delta	-
AB0R70_RS12360 (AB0R70_12360)	-	2548819..2548970 (-)	152	Protein_2387	hypothetical protein	-
AB0R70_RS12365 (AB0R70_12365)	-	2549011..2549145 (+)	135	WP_003152870.1	YqzM family protein	-
AB0R70_RS12370 (AB0R70_12370)	comEC	2549164..2551515 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB0R70_RS12375 (AB0R70_12375)	-	2551516..2552085 (-)	570	WP_094031848.1	ComE operon protein 2	-
AB0R70_RS12380 (AB0R70_12380)	comEA	2552152..2552766 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB0R70_RS12385 (AB0R70_12385)	comER	2552825..2553646 (+)	822	WP_012118027.1	late competence protein ComER	-
AB0R70_RS12390 (AB0R70_12390)	-	2553715..2554452 (-)	738	WP_076424998.1	class I SAM-dependent methyltransferase	-
AB0R70_RS12395 (AB0R70_12395)	rsfS	2554449..2554805 (-)	357	WP_007408260.1	ribosome silencing factor	-
AB0R70_RS12400 (AB0R70_12400)	yqeK	2554823..2555383 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB0R70_RS12405 (AB0R70_12405)	-	2555373..2555942 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
AB0R70_RS12410 (AB0R70_12410)	yhbY	2555953..2556243 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
AB0R70_RS12415 (AB0R70_12415)	aroE	2556237..2557079 (-)	843	WP_059368084.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=1019100 AB0R70_RS12380 WP_043021637.1 2552152..2552766(-) (comEA) [Bacillus velezensis strain JM204]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1019100 AB0R70_RS12380 WP_043021637.1 2552152..2552766(-) (comEA) [Bacillus velezensis strain JM204]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363