Detailed information NTG1018948

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB0R71_RS12375
• Genome accession: NZ_CP160210
• Coordinates: 2552143..2552757 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JM907
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2547143..2557757

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0R71_RS12345 (AB0R71_12350)	rpsT	2547446..2547712 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB0R71_RS12350 (AB0R71_12355)	holA	2547729..2548770 (-)	1042	Protein_2385	DNA polymerase III subunit delta	-
AB0R71_RS12355 (AB0R71_12360)	-	2548810..2548961 (-)	152	Protein_2386	hypothetical protein	-
AB0R71_RS12360 (AB0R71_12365)	-	2549002..2549136 (+)	135	WP_003152870.1	YqzM family protein	-
AB0R71_RS12365 (AB0R71_12370)	comEC	2549155..2551506 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB0R71_RS12370 (AB0R71_12375)	-	2551507..2552076 (-)	570	WP_094031848.1	ComE operon protein 2	-
AB0R71_RS12375 (AB0R71_12380)	comEA	2552143..2552757 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB0R71_RS12380 (AB0R71_12385)	comER	2552816..2553637 (+)	822	WP_012118027.1	late competence protein ComER	-
AB0R71_RS12385 (AB0R71_12390)	-	2553706..2554443 (-)	738	WP_076424998.1	class I SAM-dependent methyltransferase	-
AB0R71_RS12390 (AB0R71_12395)	rsfS	2554440..2554796 (-)	357	WP_007408260.1	ribosome silencing factor	-
AB0R71_RS12395 (AB0R71_12400)	yqeK	2554814..2555374 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB0R71_RS12400 (AB0R71_12405)	-	2555364..2555933 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
AB0R71_RS12405 (AB0R71_12410)	yhbY	2555944..2556234 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
AB0R71_RS12410 (AB0R71_12415)	aroE	2556228..2557070 (-)	843	WP_059368084.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=1018948 AB0R71_RS12375 WP_043021637.1 2552143..2552757(-) (comEA) [Bacillus velezensis strain JM907]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1018948 AB0R71_RS12375 WP_043021637.1 2552143..2552757(-) (comEA) [Bacillus velezensis strain JM907]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363