Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABXR98_RS03210 Genome accession   NZ_CP160206
Coordinates   662403..664082 (+) Length   559 a.a.
NCBI ID   WP_025329864.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 657403..669082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXR98_RS03195 (ABXR98_00620) - 658827..659429 (+) 603 WP_084585188.1 tyrosine-protein phosphatase -
  ABXR98_RS03200 (ABXR98_00615) - 659533..660507 (+) 975 WP_198190462.1 hypothetical protein -
  ABXR98_RS03205 (ABXR98_00610) recJ 660504..662201 (+) 1698 WP_374695112.1 single-stranded-DNA-specific exonuclease RecJ -
  ABXR98_RS03210 (ABXR98_00605) pilF 662403..664082 (+) 1680 WP_025329864.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABXR98_RS03215 (ABXR98_00600) pilG 664131..665363 (+) 1233 WP_025329863.1 type II secretion system F family protein Machinery gene
  ABXR98_RS03220 (ABXR98_00595) pilD 665370..666221 (+) 852 WP_370385764.1 A24 family peptidase Machinery gene
  ABXR98_RS03225 (ABXR98_00590) coaE 666218..666832 (+) 615 WP_084551530.1 dephospho-CoA kinase -
  ABXR98_RS03230 (ABXR98_00580) zapD 666838..667590 (+) 753 WP_025329860.1 cell division protein ZapD -
  ABXR98_RS03235 (ABXR98_00570) yacG 667590..667787 (+) 198 WP_025329859.1 DNA gyrase inhibitor YacG -
  ABXR98_RS03240 (ABXR98_00565) - 667834..668754 (+) 921 WP_370385763.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61890.40 Da        Isoelectric Point: 5.7840

>NTDB_id=1018857 ABXR98_RS03210 WP_025329864.1 662403..664082(+) (pilF) [Snodgrassella alvi strain Amel03]
MSLGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPALLQSGAINADKLASFLSGVFGIPYLNLEPYNQSLVPLDLINLKI
MQQYICVPLFKRGNRLFLAVSDPTSIQHYQKIATDAGCVLNLVIVRDSQLRQLLENLGQESTNLLEDMDSIGDSSAVENN
AMSADDEDGENGPIAKFVFKILGDALNSGASDIHFEFYEEMARVRFRVDGQLREVVRMPLNIRGQIASRIKVMAKLDISE
KRIPQDGRIQVQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSDEMQRIIMNNGNEVDISTQALKEGLVDLRHAGLLKVMEGITSLDEILASTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1018857 ABXR98_RS03210 WP_025329864.1 662403..664082(+) (pilF) [Snodgrassella alvi strain Amel03]
ATGAGTCTTGGACTGATCAGGGTTTTATATAAGCACCAGATTATTGACGCAGAAAAAGCAGAAAAACTGCGTCAGGAACT
GGAACAGAAACGTCCGGTTATACCGGCACTACTACAATCTGGAGCCATCAATGCTGATAAACTGGCCAGCTTTCTGTCCG
GTGTATTCGGTATTCCTTACCTGAATCTGGAGCCATATAATCAAAGTCTGGTACCGCTGGATTTAATTAATCTGAAAATT
ATGCAGCAATACATTTGCGTGCCGCTATTCAAGCGCGGTAATCGACTGTTTTTGGCTGTATCTGATCCTACATCAATTCA
GCATTACCAGAAAATTGCCACAGATGCCGGCTGCGTACTGAATCTGGTTATAGTCCGCGATAGTCAGTTACGCCAATTAC
TGGAAAATCTTGGTCAGGAATCCACCAACCTTTTAGAAGATATGGATTCAATTGGTGACTCCAGTGCTGTAGAAAATAAC
GCCATGTCTGCAGATGACGAAGATGGCGAAAATGGACCAATTGCAAAATTTGTTTTTAAAATACTAGGTGATGCATTAAA
CAGCGGCGCATCAGATATCCATTTTGAATTTTATGAAGAAATGGCACGAGTACGCTTTCGCGTTGACGGACAGTTGCGTG
AAGTGGTACGTATGCCACTGAATATTCGCGGCCAGATCGCTTCACGTATTAAAGTAATGGCCAAACTGGACATTTCAGAA
AAACGTATTCCTCAGGACGGACGTATTCAGGTTCAATTCCGTAAAGGTGCCAAGCCGATTGATTTCCGTGTCAGCACACT
GCCCACTCTGTTCGGGGAAAAAATTGTAATGCGTATCCTGAATTCGGATGCGGCTACTCTGAATATTGAACAACTTGGTT
TTGAGCCATTTCAGAAAGAATTACTGCTTGAGGCCATCCACCGGCCATACGGTATGGTGCTGGTTACCGGCCCTACTGGT
TCCGGTAAAACCGTATCACTGTATACCTGCCTTAATATTCTTAATACCGACAGTGTTAACATTTCCACTGCTGAAGACCC
TGCGGAAATTAACCTGCCTGGTATTAATCAGGTAAATGTTAATGATAAACAAGGACTGACATTTGCTGCTGCGTTAAAAT
CATTTCTGCGCCAGGATCCGGATATCATTATGGTGGGGGAAATCCGAGATCTGGAAACCGCAGATATTGCCATTAAAGCT
GCACAAACCGGCCATATGGTATTTTCAACTCTGCATACCAATAATGCCCCTGCTACATTATCCCGTATGCTGAATATGGG
CGTTGCTCCGTTTAATATTGCCAGCTCAGTAAATCTGATTATGGCACAGCGTCTGGTGCGGCGTTTATGTACCTGCAAGG
CACCGGCAGAGCGTCCGCCTGAATTTGCACTGAAAAAAGCAGGTTTTTCAGATGAAGATCTCGCCAAAGAATGGACATTT
TATCGGCCGGTTGGCTGTGAAAGGTGTAAGGGCAAAGGCTATAAAGGACGTGCCGGCGTATATGAAGTTATGCCGGTCAG
TGATGAAATGCAACGTATTATCATGAATAATGGTAATGAAGTAGATATCAGCACTCAGGCGCTCAAGGAAGGTCTGGTCG
ATTTACGCCATGCCGGTTTACTGAAAGTGATGGAAGGCATTACGTCTCTGGACGAGATTCTTGCAAGCACTAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

67.857

100

0.68
  pilB Acinetobacter baylyi ADP1

51.601

100

0.519
  pilB Acinetobacter baumannii D1279779

52.741

94.633

0.499
  pilB Legionella pneumophila strain ERS1305867

49.033

100

0.499
  pilB Vibrio cholerae strain A1552

44.92

100

0.451
  pilB Vibrio campbellii strain DS40M4

44.405

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

43.816

100

0.444
  pilF Thermus thermophilus HB27

40.299

95.886

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.341

94.454

0.381

Multiple sequence alignment