Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABX025_RS07575 Genome accession   NZ_CP160073
Coordinates   1660903..1662582 (+) Length   559 a.a.
NCBI ID   WP_371751717.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1655903..1667582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABX025_RS07560 (ABX025_14335) - 1657395..1657931 (+) 537 WP_371751719.1 tyrosine-protein phosphatase -
  ABX025_RS07565 (ABX025_14340) - 1658034..1659008 (+) 975 WP_369432851.1 hypothetical protein -
  ABX025_RS07570 (ABX025_14345) recJ 1659005..1660702 (+) 1698 WP_371751718.1 single-stranded-DNA-specific exonuclease RecJ -
  ABX025_RS07575 (ABX025_14355) pilF 1660903..1662582 (+) 1680 WP_371751717.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABX025_RS07580 (ABX025_14360) pilG 1662629..1663861 (+) 1233 WP_084547381.1 type II secretion system F family protein Machinery gene
  ABX025_RS07585 (ABX025_14365) pilD 1663868..1664719 (+) 852 WP_367449354.1 A24 family peptidase Machinery gene
  ABX025_RS07590 (ABX025_14370) coaE 1664716..1665330 (+) 615 WP_078475026.1 dephospho-CoA kinase -
  ABX025_RS07595 (ABX025_14375) zapD 1665336..1666088 (+) 753 WP_025329860.1 cell division protein ZapD -
  ABX025_RS07600 (ABX025_14380) yacG 1666088..1666285 (+) 198 WP_078475027.1 DNA gyrase inhibitor YacG -
  ABX025_RS07605 (ABX025_14385) - 1666332..1667252 (+) 921 WP_025329858.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61957.49 Da        Isoelectric Point: 5.7531

>NTDB_id=1017622 ABX025_RS07575 WP_371751717.1 1660903..1662582(+) (pilF) [Snodgrassella alvi strain Amel09]
MSLGLIRVLYKHQIIDAEKAEKLRQELEQKHPVIPALLQSGVISADKLASFLSGVFGIPYLNLEPYNQNLVPLDLINLKI
MQQYICVPLFKRGNRLFLAVSDPTSIQHYQKIATDAGCVLNLVIVRDSQLRQLLENLGQESTTLLEDMDSIGDSSAVENN
AMSADDEDGENGPIAKFVFKILGDALNSGASDIHFEFYEEMARVRFRVDGQLREVVRMPLNIRSQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSDEMQRIIMNNGNEVDISNQALKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1017622 ABX025_RS07575 WP_371751717.1 1660903..1662582(+) (pilF) [Snodgrassella alvi strain Amel09]
ATGAGTCTTGGACTGATCAGGGTTTTATATAAGCACCAGATTATTGACGCAGAAAAAGCAGAAAAGCTGCGTCAGGAACT
GGAACAGAAGCATCCGGTTATACCGGCACTACTACAATCCGGAGTCATCAGTGCTGATAAACTGGCCAGCTTTCTGTCCG
GTGTATTCGGTATTCCTTACCTGAATCTGGAGCCATATAACCAAAATCTGGTACCGTTGGATCTAATTAATCTGAAAATT
ATGCAGCAATACATTTGCGTGCCGCTATTCAAGCGCGGTAACCGACTGTTTTTAGCTGTATCTGATCCTACATCAATTCA
GCATTACCAGAAAATTGCCACAGATGCCGGCTGCGTACTGAATCTGGTTATAGTCCGCGATAGTCAGTTACGCCAATTAC
TGGAAAATCTTGGTCAGGAATCCACCACCCTTCTGGAAGATATGGATTCAATTGGTGACTCCAGTGCTGTAGAAAATAAC
GCCATGTCTGCAGATGACGAAGATGGCGAAAATGGACCAATTGCAAAATTTGTTTTTAAAATACTAGGTGATGCATTAAA
TAGCGGCGCATCAGATATCCATTTTGAATTTTATGAAGAAATGGCACGAGTACGCTTTCGCGTTGACGGACAGTTGCGTG
AAGTGGTACGTATGCCACTGAATATTCGCAGCCAGATCGCTTCACGTATTAAAGTGATGGCCAAACTGGATATTTCAGAA
AAACGTATTCCTCAGGACGGACGTATTCAGATTCAGTTCCGTAAAGGTGCCAAGCCAATTGATTTCCGTGTCAGCACACT
GCCCACTCTGTTTGGGGAAAAAATCGTAATGCGTATCCTGAATTCGGATGCGGCTACTCTGAATATTGAACAACTTGGTT
TTGAGCCATTTCAGAAAGAATTACTGCTTGAAGCCATCCACCGGCCATACGGTATGGTGCTGGTTACCGGCCCTACCGGT
TCCGGTAAAACCGTATCACTGTATACCTGCCTTAATATTCTTAATACCGACAGTGTTAACATTTCCACTGCTGAAGACCC
TGCGGAAATTAACCTGCCCGGTATTAATCAGGTAAATGTTAATGATAAACAGGGACTGACATTTGCTGCAGCGTTAAAAT
CATTTCTGCGCCAGGATCCGGATATCATTATGGTGGGGGAAATCCGTGATTTGGAAACCGCAGATATTGCCATTAAAGCT
GCACAAACCGGCCATATGGTATTTTCAACTCTGCATACCAATAATGCCCCTGCTACATTATCCCGTATGCTGAATATGGG
CGTTGCTCCGTTTAATATTGCCAGCTCAGTAAATCTGATTATGGCACAGCGTCTGGTGCGGCGTTTATGTACCTGCAAGG
CACCGGCAGAGCGTCCGCCTGAATTCGCACTGAAAAAAGCAGGTTTTTCAGATGAAGATCTCGCCAAAGAATGGACATTT
TATCGGCCGGTCGGCTGTGAAAGGTGTAAGGGTAAAGGCTATAAAGGACGTGCCGGCGTATATGAAGTTATGCCGGTCAG
TGATGAAATGCAACGTATTATCATGAATAATGGTAATGAAGTTGATATCAGCAATCAGGCACTCAAGGAAGGGCTAGTTG
ATTTACGTCATGCCGGTTTACTGAAAGTCATGGAAGGCATAACGTCTCTGGATGAAATTCTGGCAACCACCAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.036

100

0.682
  pilB Acinetobacter baylyi ADP1

51.408

100

0.522
  pilB Legionella pneumophila strain ERS1305867

49.385

100

0.503
  pilB Acinetobacter baumannii D1279779

52.336

95.707

0.501
  pilB Vibrio campbellii strain DS40M4

44.405

100

0.447
  pilB Vibrio cholerae strain A1552

44.563

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

43.816

100

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.678

94.991

0.386
  pilF Thermus thermophilus HB27

40.112

95.886

0.385

Multiple sequence alignment