Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABX022_RS04415 Genome accession   NZ_CP160072
Coordinates   1011307..1012986 (-) Length   559 a.a.
NCBI ID   WP_198224852.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1006307..1017986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABX022_RS04385 (ABX022_02335) - 1006637..1007557 (-) 921 WP_025329858.1 ABC transporter ATP-binding protein -
  ABX022_RS04390 (ABX022_02330) yacG 1007604..1007801 (-) 198 WP_078475027.1 DNA gyrase inhibitor YacG -
  ABX022_RS04395 (ABX022_02325) zapD 1007801..1008553 (-) 753 WP_025329860.1 cell division protein ZapD -
  ABX022_RS04400 (ABX022_02320) coaE 1008559..1009173 (-) 615 WP_084565125.1 dephospho-CoA kinase -
  ABX022_RS04405 (ABX022_02315) pilD 1009170..1010021 (-) 852 WP_198224850.1 A24 family peptidase Machinery gene
  ABX022_RS04410 (ABX022_02310) pilG 1010028..1011260 (-) 1233 WP_370580578.1 type II secretion system F family protein Machinery gene
  ABX022_RS04415 (ABX022_02305) pilF 1011307..1012986 (-) 1680 WP_198224852.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABX022_RS04420 (ABX022_02300) recJ 1013187..1014884 (-) 1698 WP_367483178.1 single-stranded-DNA-specific exonuclease RecJ -
  ABX022_RS04425 (ABX022_15440) - 1014881..1015855 (-) 975 WP_198224856.1 hypothetical protein -
  ABX022_RS04430 (ABX022_02285) - 1015958..1016560 (-) 603 WP_370580579.1 tyrosine-protein phosphatase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61915.41 Da        Isoelectric Point: 5.7531

>NTDB_id=1017588 ABX022_RS04415 WP_198224852.1 1011307..1012986(-) (pilF) [Snodgrassella alvi strain Amel07]
MSLGLIRVLYKHQIIDAEKAEKLRQELEQKHPVIPALLQSGAISADKLASFLSGVFGIPYLNLEPYNQNLVPLDLINLKI
MQQYICVPLFKRGNRLFLAVSDPTSIQHYQKIATDAGCVLNLVIVRDSQLRQLLENLGQESTTLLEDMDSIGDSSAVENN
AMSADDEDGENGPIAKFVFKILGDALNSGASDIHFEFYEEMARVRFRVDGQLREVVRMPLNIRSQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAASLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSDEMQRIIMNNGNEVDISNQALKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1017588 ABX022_RS04415 WP_198224852.1 1011307..1012986(-) (pilF) [Snodgrassella alvi strain Amel07]
ATGAGTCTTGGACTGATCAGGGTTTTATATAAGCACCAGATTATTGACGCAGAAAAAGCAGAAAAGCTGCGTCAGGAACT
GGAACAGAAACATCCGGTTATACCGGCATTACTACAATCTGGAGCCATCAGTGCTGATAAACTGGCCAGCTTTCTGTCCG
GTGTATTCGGTATTCCTTACCTGAATCTGGAGCCATATAACCAAAATCTGGTACCGTTGGATCTAATTAATCTGAAAATT
ATGCAGCAATACATTTGCGTGCCGCTATTCAAGCGCGGTAATCGACTGTTTTTAGCTGTATCTGATCCTACATCAATTCA
GCATTACCAGAAAATTGCCACAGATGCCGGCTGCGTACTGAATCTGGTTATAGTCCGCGATAGTCAGTTACGCCAATTAC
TGGAAAATCTTGGTCAGGAATCCACCACCCTTCTGGAAGATATGGATTCAATTGGTGACTCCAGTGCTGTAGAAAATAAC
GCCATGTCTGCAGATGACGAAGATGGCGAAAATGGACCAATTGCAAAATTTGTTTTTAAAATACTTGGAGATGCATTAAA
TAGCGGCGCATCAGATATCCATTTTGAATTTTATGAAGAAATGGCACGAGTACGCTTTCGCGTTGACGGACAGTTGCGTG
AAGTGGTACGTATGCCACTGAATATTCGCAGCCAGATCGCTTCACGTATTAAAGTGATGGCCAAACTGGATATTTCAGAA
AAACGTATTCCTCAGGACGGACGTATTCAGATTCAGTTCCGTAAAGGTGCCAAACCAATTGATTTCCGTGTCAGCACACT
GCCCACTCTGTTTGGGGAAAAAATCGTAATGCGTATCCTGAATTCGGATGCGGCTAGTCTGAATATTGAACAACTTGGTT
TTGAGCCATTTCAGAAAGAATTACTGCTTGAGGCCATCCACCGACCATACGGTATGGTGTTGGTTACCGGCCCTACCGGT
TCCGGTAAAACCGTATCACTATATACCTGCCTTAATATTCTTAATACCGATAGTGTTAACATTTCCACTGCTGAAGACCC
TGCGGAAATTAACCTGCCCGGTATTAATCAGGTAAATGTTAATGATAAACAGGGACTGACATTCGCTGCAGCGTTAAAAT
CATTTCTGCGCCAGGATCCGGATATCATTATGGTGGGGGAAATCCGTGATTTGGAAACCGCAGATATTGCCATTAAAGCT
GCACAAACCGGCCATATGGTATTTTCAACTCTGCATACCAATAATGCCCCTGCTACATTATCCCGTATGCTGAATATGGG
CGTTGCTCCGTTTAATATTGCCAGCTCAGTAAATCTGATTATGGCACAGCGTCTGGTGCGGCGTTTATGTACCTGCAAGG
CACCGGCAGAGCGTCCGCCTGAATTTGCACTGAAAAAAGCAGGTTTTTCAGATGAAGATCTCGCCAAAGAATGGACATTT
TACCGGCCGGTCGGCTGTGAAAGGTGTAAGGGTAAAGGCTATAAAGGACGTGCCGGCGTATATGAAGTTATGCCGGTCAG
TGATGAAATGCAACGTATTATCATGAATAATGGTAATGAAGTTGATATCAGCAATCAGGCACTCAAGGAAGGGCTAGTTG
ATTTACGTCATGCCGGTTTACTGAAAGTCATGGAAGGCATAACGTCTCTGGATGAAATTCTGGCAACCACCAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.036

100

0.682
  pilB Acinetobacter baylyi ADP1

51.408

100

0.522
  pilB Legionella pneumophila strain ERS1305867

49.385

100

0.503
  pilB Acinetobacter baumannii D1279779

52.336

95.707

0.501
  pilB Vibrio campbellii strain DS40M4

44.405

100

0.447
  pilB Vibrio cholerae strain A1552

44.563

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

43.993

100

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.678

94.991

0.386
  pilF Thermus thermophilus HB27

40.112

95.886

0.385

Multiple sequence alignment