Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABZM26_RS22990 Genome accession   NZ_CP160044
Coordinates   5100168..5101868 (+) Length   566 a.a.
NCBI ID   WP_056790439.1    Uniprot ID   A0AAQ3G636
Organism   Pseudomonas atacamensis strain GZ-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5095168..5106868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZM26_RS22970 - 5095368..5098319 (+) 2952 WP_123443322.1 DUF748 domain-containing protein -
  ABZM26_RS22975 - 5098316..5098615 (+) 300 WP_071173628.1 DUF2845 domain-containing protein -
  ABZM26_RS22980 - 5098851..5099204 (-) 354 WP_016773155.1 BON domain-containing protein -
  ABZM26_RS22985 - 5099504..5099941 (-) 438 WP_122698547.1 pilin -
  ABZM26_RS22990 pilB 5100168..5101868 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABZM26_RS22995 pilC 5101871..5103088 (+) 1218 WP_366300579.1 type II secretion system F family protein Machinery gene
  ABZM26_RS23000 pilD 5103090..5103962 (+) 873 WP_123443320.1 A24 family peptidase Machinery gene
  ABZM26_RS23005 coaE 5103959..5104582 (+) 624 WP_123443319.1 dephospho-CoA kinase -
  ABZM26_RS23010 yacG 5104579..5104779 (+) 201 WP_366300580.1 DNA gyrase inhibitor YacG -
  ABZM26_RS23015 - 5104787..5105002 (-) 216 WP_056790451.1 hypothetical protein -
  ABZM26_RS23020 - 5105068..5105757 (-) 690 WP_366300581.1 energy-coupling factor ABC transporter permease -
  ABZM26_RS23025 - 5105875..5106501 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62620.67 Da        Isoelectric Point: 6.1319

>NTDB_id=1017288 ABZM26_RS22990 WP_056790439.1 5100168..5101868(+) (pilB) [Pseudomonas atacamensis strain GZ-3]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1017288 ABZM26_RS22990 WP_056790439.1 5100168..5101868(+) (pilB) [Pseudomonas atacamensis strain GZ-3]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAACAAGTGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGCGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAAATTG
CCGAGATCGCCTCGGAACATTTCGGCATGGCCCTGCTCGATCTGCATTGCCTCGACAAGGAGACTCAGCCCAAGGGGCTG
GTCAGTGAAAAACTGGTTCGTCAGCATTGCGCCCTGCCCTTGTGGCGGCGCGGCAACAAACTGTTCGTGGGCCTTTCCGA
CCCGAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTCGAGGACGACA
AACTCAGCGACGCCATCGACAAGTTCTTCGACAGCCACGCCTCGGGTCTGGAAGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATCGATGACAACAAACAGGACGTGATCGGCGGCCTCGATGCCGACGACGCCCCGGTCGTGCGCTT
CGTGCACAAGATGCTGCTCGATGCGATCAAGAGTGGCTCTTCCGACCTGCATTTCGAGCCGTACGAAAAAAACTACCGGG
TGCGCATGCGCACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATCCAGCTGGCCGGGCGTATTGCCGCTCGCCTG
AAGGTGATGGCCAGTCTGGATATTTCGGAACGACGCAAACCCCAGGACGGACGGATCAAGATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CGCAAATGGGCATCGACGCATTGGGTTACGAGGCCGAACAAAAGGCCTTGTACCTGGCCGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACCGGCCCCACCGGCTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCCGCCAAG
GGCTGGACTTCGCCCAGGCGCTGCGCTCATTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCAATCAAGGCTGCGCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCGGCGGA
AACCCTCACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATTGCCACCTCGGTCAGCCTGATCATCGCCCAGCGGC
TGGCGCGCAAATTGTGCGCGCACTGCAAGCGACCGCTGGAAATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTGAAGAACACACCGGACCTGCAACGGTTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACATCCAGA
TGCGCCGCGACGGTTTCGCCGATCTGCGCACTTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

99.117

0.5
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.837

94.17

0.366

Multiple sequence alignment