Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABSY19_RS08535 Genome accession   NZ_CP159956
Coordinates   1810637..1812370 (+) Length   577 a.a.
NCBI ID   WP_382161347.1    Uniprot ID   -
Organism   Hydrogenophaga sp. ANAO-22     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1805637..1817370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABSY19_RS08515 (ABSY19_08510) - 1806950..1807207 (-) 258 Protein_1688 IS21 family transposase -
  ABSY19_RS08520 (ABSY19_08515) - 1807315..1809135 (-) 1821 Protein_1689 DEAD/DEAH box helicase family protein -
  ABSY19_RS08525 (ABSY19_08520) - 1809132..1809371 (-) 240 WP_382161341.1 helix-turn-helix domain-containing protein -
  ABSY19_RS08530 (ABSY19_08525) - 1809646..1809969 (-) 324 WP_382161344.1 helix-turn-helix transcriptional regulator -
  ABSY19_RS08535 (ABSY19_08530) pilB 1810637..1812370 (+) 1734 WP_382161347.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABSY19_RS08540 (ABSY19_08535) pilC 1812412..1813629 (+) 1218 WP_291121454.1 type II secretion system F family protein Machinery gene
  ABSY19_RS08545 (ABSY19_08540) - 1813629..1814492 (+) 864 WP_382161350.1 prepilin peptidase -
  ABSY19_RS08550 (ABSY19_08545) coaE 1814516..1815103 (+) 588 WP_382161353.1 dephospho-CoA kinase -
  ABSY19_RS08555 (ABSY19_08550) zapD 1815147..1815902 (+) 756 WP_291121446.1 cell division protein ZapD -
  ABSY19_RS08560 (ABSY19_08555) - 1815911..1816114 (+) 204 WP_382161355.1 DNA gyrase inhibitor YacG -
  ABSY19_RS08565 (ABSY19_08560) - 1816145..1816597 (-) 453 WP_382161358.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62657.09 Da        Isoelectric Point: 5.6281

>NTDB_id=1016887 ABSY19_RS08535 WP_382161347.1 1810637..1812370(+) (pilB) [Hydrogenophaga sp. ANAO-22]
MASVETVPQETPSMALPGLGRALVSAGKLGQKAAEDLYRKAQSGRSSFIAELTGSGAVSASDLAHTMSVAFAAPLLDLDA
IDVQRLPAGLLDPKICSDYRIVVLSKRNNRLMVATADPADQQAAEKIKFATQMGVDWVIAEYDKLSKLVETQTTSVNDTM
DNIIGGEFEFDEAATETSVSDVTDKASEVDDAPVVKFLHKMLIDAFNMRASDLHFEPYEHNYRVRFRVDGELREIASPPI
VIKDKLASRIKVISRMDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKVGIDALGYEPEEKER
LLNAISRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKAFLRQDPD
VIMVGEIRDLETADISIKAAQTGHMVLSTLHTNDAPTTLTRMMNMGIPTFNIASSVILITAQRLARRLCATCKAPLDVPR
KALVDAGFKPEDLDGTWTPYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRGGSALDIAQQAGKEGVRTLRESGLLK
VKLGMTSLEEVLSVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1016887 ABSY19_RS08535 WP_382161347.1 1810637..1812370(+) (pilB) [Hydrogenophaga sp. ANAO-22]
ATGGCGTCCGTCGAAACCGTCCCCCAGGAGACCCCGTCCATGGCCTTGCCAGGCCTGGGCCGCGCGCTCGTGTCTGCGGG
GAAGCTTGGCCAGAAGGCGGCTGAAGATCTTTATCGCAAAGCACAAAGCGGCCGCTCCAGCTTCATCGCAGAACTGACCG
GCTCAGGGGCTGTGTCCGCTTCGGATCTGGCCCACACCATGTCGGTGGCTTTTGCCGCGCCACTGCTCGATCTGGACGCG
ATCGACGTGCAACGCCTGCCCGCCGGCCTGCTCGATCCCAAGATCTGCAGCGACTACCGCATCGTCGTCCTGAGCAAACG
CAACAACCGCTTGATGGTGGCCACGGCCGATCCAGCCGACCAACAGGCCGCCGAGAAGATCAAGTTCGCGACCCAGATGG
GCGTGGACTGGGTGATTGCCGAGTACGACAAGCTCAGCAAACTGGTGGAGACCCAGACCACCAGCGTCAACGACACGATG
GACAACATCATCGGTGGCGAGTTCGAGTTCGACGAGGCGGCCACCGAGACGAGCGTGAGCGACGTGACCGACAAGGCGTC
GGAAGTCGACGACGCGCCCGTGGTCAAGTTCCTGCACAAGATGCTGATCGACGCATTCAACATGCGGGCATCCGACTTGC
ACTTTGAGCCTTACGAGCACAACTACCGGGTCCGTTTTCGGGTGGACGGCGAACTGCGTGAAATTGCGTCGCCGCCGATC
GTGATCAAGGACAAGCTGGCGTCGCGGATCAAGGTGATCTCCCGCATGGACATCTCGGAGAAGCGTGTGCCCCAGGACGG
GCGCATGAAACTCAAGATCGGACCGGACCGCGTGATCGACTTTCGGGTCAGCACCCTGCCCACCTTGTTCGGCGAGAAGA
TCGTGATCCGTATTCTGGACCCGAGCAGCGCCAAGGTCGGCATCGATGCCCTGGGCTACGAACCCGAGGAAAAAGAGCGG
CTGTTGAACGCGATCAGCCGGCCATACGGCATGGTGTTGGTCACCGGCCCCACGGGGTCGGGGAAAACTGTGTCGCTGTA
CACCTGCCTGAACATCCTGAACAAGCCAGGCATCAACATTTCCACGGCGGAAGATCCGTCGGAAATCAACCTGCCGGGTG
TCAATCAGGTCAACGTCAACGAGAAGGCGGGCCTGACCTTCGCGGCGGCCCTCAAGGCATTTCTGCGCCAGGATCCCGAC
GTGATCATGGTGGGTGAGATTCGCGATCTCGAAACGGCGGACATCTCGATCAAGGCCGCACAAACCGGTCACATGGTGCT
GTCCACCCTGCACACGAACGACGCGCCGACCACACTGACGCGGATGATGAACATGGGCATTCCCACGTTCAACATCGCCT
CCAGCGTGATCCTGATCACCGCCCAGCGGCTGGCCCGCCGCCTGTGCGCCACCTGCAAGGCGCCGCTGGACGTACCGCGC
AAGGCCCTGGTGGACGCCGGATTCAAGCCGGAAGACCTTGATGGCACCTGGACGCCCTACAAGCCCGTGGGGTGCTCGGC
CTGCAACAACGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCCATTTCGGAAGAAATCCAACGCATCATCCTGC
GTGGTGGCAGCGCGCTCGACATTGCCCAGCAGGCCGGCAAGGAGGGCGTTCGCACGCTGCGCGAATCGGGTCTGCTCAAG
GTGAAGCTGGGCATGACTTCGCTGGAAGAAGTGCTCAGCGTGACCAACGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.345

100

0.539
  pilB Acinetobacter baylyi ADP1

54.189

97.227

0.527
  pilF Neisseria gonorrhoeae MS11

52.827

98.094

0.518
  pilB Legionella pneumophila strain ERS1305867

49.737

98.96

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

45.714

97.054

0.444
  pilB Vibrio cholerae strain A1552

48.369

90.295

0.437
  pilB Vibrio campbellii strain DS40M4

43.915

98.267

0.432
  pilF Thermus thermophilus HB27

37.961

98.614

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.696

99.307

0.374

Multiple sequence alignment