Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABU614_RS23125 Genome accession   NZ_CP159925
Coordinates   5321948..5323117 (-) Length   389 a.a.
NCBI ID   WP_064749330.1    Uniprot ID   A0AAU8MWI3
Organism   Lysobacter firmicutimachus strain SR10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5316948..5328117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU614_RS23125 (ABU614_23125) pilU 5321948..5323117 (-) 1170 WP_064749330.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABU614_RS23130 (ABU614_23130) pilT 5323236..5324273 (-) 1038 WP_336132370.1 type IV pilus twitching motility protein PilT Machinery gene
  ABU614_RS23135 (ABU614_23135) - 5324448..5325125 (+) 678 WP_363800962.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABU614_RS23140 (ABU614_23140) proC 5325268..5326104 (+) 837 WP_064749333.1 pyrroline-5-carboxylate reductase -
  ABU614_RS23145 (ABU614_23145) - 5326101..5326571 (+) 471 WP_336132373.1 DUF4426 domain-containing protein -
  ABU614_RS23150 (ABU614_23150) - 5326826..5327620 (+) 795 WP_336132374.1 cobalamin-binding protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43113.50 Da        Isoelectric Point: 6.8285

>NTDB_id=1016831 ABU614_RS23125 WP_064749330.1 5321948..5323117(-) (pilU) [Lysobacter firmicutimachus strain SR10]
MNNPAPNAPASPATGAIDFTSFLKLMAHQRASDLFITAGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMNPQQREEFE
KTHECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMI
GYRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLV
LCTLHANNANQAMDRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKE
LMKDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=1016831 ABU614_RS23125 WP_064749330.1 5321948..5323117(-) (pilU) [Lysobacter firmicutimachus strain SR10]
ATGAACAACCCCGCACCCAACGCTCCGGCCAGCCCTGCGACCGGCGCCATCGACTTCACCTCCTTCCTCAAGCTGATGGC
GCACCAGCGCGCCTCGGACTTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCAGCCCGATCA
CCCAGAACCCGCTGACGCCGCAGCAGAGCCGCGATCTCGTGCTGAACGTGATGAATCCGCAGCAGCGCGAAGAGTTCGAA
AAAACCCACGAGTGCAACTTCGCCATCGGCGTGACCGGCGTCGGCCGCTTCCGCGTCAGCTGCTTCTATCAGCGCAATCA
GGTCGGCATGGTGCTGCGCCGGATCGAAACCAAGATCCCGACCGTCGAAGAGCTGAACCTGCCGCCGATCATCAAGACGC
TGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGTACCGGTAAGTCGACTTCGCTGGCGGCGATGATC
GGCTACCGCAACCAGAATTCGACCGGACACATCATCACCATCGAAGACCCGATCGAATTCGTGCACCGCCACGAGGGTTG
CATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGACGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGG
ACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTCGTG
CTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGTCGCGGCCA
GTTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGATCCCGACTCCGGACGGCAAGGGCCGGC
GCGTGGCGATGGAAATCCTGCTCGGCACCCCGCTGGCCCAGGACTACATCCGTGACGGCGAGATCCACAAGCTCAAGGAA
CTGATGAAGGACTCCGTGCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTATCAGGCCGGCGAGATCAG
CTACGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGATGCGC
GGACGCTGTCGCAGGGGTTGGATGGCGTCGAAGTGGCCGAAGTGCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(131-285)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.937

94.344

0.65
  pilU Acinetobacter baylyi ADP1

64.146

91.774

0.589
  pilU Vibrio cholerae strain A1552

53.09

91.517

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.735

90.231

0.386

Multiple sequence alignment