Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABV523_RS00645 Genome accession   NZ_CP159758
Coordinates   133579..135258 (+) Length   559 a.a.
NCBI ID   WP_267408994.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Acer02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 128579..140258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABV523_RS00630 (ABV523_16115) - 128896..130662 (+) 1767 WP_369608788.1 M61 family metallopeptidase -
  ABV523_RS00635 (ABV523_10330) - 130711..131685 (+) 975 WP_369608787.1 hypothetical protein -
  ABV523_RS00640 (ABV523_10325) recJ 131676..133379 (+) 1704 WP_369608786.1 single-stranded-DNA-specific exonuclease RecJ -
  ABV523_RS00645 (ABV523_10320) pilF 133579..135258 (+) 1680 WP_267408994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABV523_RS00650 (ABV523_10315) pilG 135305..136537 (+) 1233 WP_100156468.1 type II secretion system F family protein Machinery gene
  ABV523_RS00655 (ABV523_10310) pilD 136544..137395 (+) 852 WP_369608785.1 A24 family peptidase Machinery gene
  ABV523_RS00660 (ABV523_10305) coaE 137392..138006 (+) 615 WP_100156466.1 dephospho-CoA kinase -
  ABV523_RS00665 (ABV523_10300) zapD 138012..138764 (+) 753 WP_367430673.1 cell division protein ZapD -
  ABV523_RS00670 (ABV523_10295) yacG 138764..138961 (+) 198 WP_100140841.1 DNA gyrase inhibitor YacG -
  ABV523_RS00675 (ABV523_10290) - 139011..139928 (+) 918 WP_100156465.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62245.82 Da        Isoelectric Point: 5.9508

>NTDB_id=1015658 ABV523_RS00645 WP_267408994.1 133579..135258(+) (pilF) [Snodgrassella alvi strain Acer02]
MSVGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPMLLQSGVIDADKLASFLSKVFSIPYLNLELYNQSLVPQDLINLKI
MQQYICVPIFKRGNRLFLAVSDPTSIQHYQKIATDAGCILNLVIVRDDQLRQILENLGQESTSLLEGMDTVGDAEATGNN
AMSADDEEGENGPVARFVFKVLNDALNSGASDIHFEFYEEMARVRFRIDGQLREVVRMPLNIRNQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPISDEMQRIIMNNGNEVDISQQAHKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1015658 ABV523_RS00645 WP_267408994.1 133579..135258(+) (pilF) [Snodgrassella alvi strain Acer02]
ATGAGTGTAGGATTGATTCGAGTTTTATATAAGCACCAGATTATCGATGCAGAAAAAGCTGAAAAACTGCGTCAGGAGCT
TGAGCAGAAACGTCCAGTTATACCGATGCTGTTGCAGTCTGGTGTCATCGATGCTGATAAACTGGCCAGCTTCCTGTCCA
AGGTATTCAGTATTCCTTACCTCAATCTGGAACTATATAACCAAAGCCTTGTACCGCAGGACTTAATCAATCTGAAAATC
ATGCAGCAATATATCTGCGTGCCCATTTTCAAACGTGGAAATCGTCTGTTTCTGGCTGTATCTGACCCCACTTCCATTCA
GCATTATCAGAAAATTGCCACAGATGCCGGCTGCATACTGAATCTGGTCATTGTACGCGATGATCAGCTTCGTCAGATAC
TAGAAAATCTCGGACAGGAATCCACCAGCCTACTAGAAGGAATGGACACAGTTGGTGATGCTGAAGCAACAGGCAATAAT
GCCATGTCTGCCGATGATGAGGAGGGCGAAAACGGCCCTGTGGCCAGATTTGTATTCAAAGTTTTGAATGATGCACTCAA
CAGCGGCGCCTCAGATATTCACTTTGAATTTTACGAAGAAATGGCACGAGTACGTTTTCGTATTGACGGGCAGCTACGGG
AAGTAGTGCGTATGCCACTGAATATTAGAAACCAGATTGCCTCCCGTATTAAAGTGATGGCTAAACTGGACATTTCAGAA
AAACGTATACCTCAGGATGGTCGAATTCAGATTCAATTCCGCAAAGGCGCCAAACCGATTGATTTTCGTGTCAGTACTCT
TCCGACCCTGTTTGGTGAAAAAATTGTGATGCGTATTCTGAATTCGGATGCCGCTACCCTGAATATCGAACAGCTCGGCT
TCGAACCATTTCAGAAAGAATTACTGCTGGAAGCCATTCACCGGCCGTATGGTATGGTACTGGTTACCGGTCCAACCGGT
TCCGGTAAAACTGTATCCCTTTACACCTGCCTGAATATTCTGAATACCGACAGTGTGAACATTTCTACTGCAGAAGATCC
TGCGGAAATTAATTTACCCGGTATCAATCAGGTCAATGTAAATGATAAACAGGGATTAACATTTGCCGCAGCTCTAAAAT
CTTTCCTACGTCAGGATCCAGACATCATCATGGTGGGGGAAATCCGTGATTTAGAAACGGCCGATATTGCCATCAAAGCA
GCACAAACCGGTCATATGGTATTTTCTACCCTGCACACCAATAACGCGCCTGCCACCTTATCACGTATGCTGAATATGGG
AGTTGCTCCATTCAACATCGCAAGTTCAGTGAATCTGATTATGGCACAGCGTTTGGTTCGACGTTTATGTACCTGCAAAG
CACCAGCCGAACGGCCACCAGAATTCGCACTGAAAAAAGCCGGTTTCTCTGATGAAGATCTAGCTAAAGAATGGACATTT
TATCGACCTGTAGGCTGTGAGCGCTGTAAAGGCAAAGGTTACAAGGGGCGCGCTGGTGTTTATGAAGTAATGCCTATCAG
TGATGAAATGCAGCGAATCATCATGAACAATGGTAATGAAGTCGATATCAGTCAGCAGGCGCATAAAGAAGGACTGGTCG
ATTTGCGTCATGCTGGTCTGCTTAAAGTTATGGAGGGCATCACTTCGCTTGACGAAATTCTAGCCACCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.107

100

0.692
  pilB Acinetobacter baylyi ADP1

50.528

100

0.513
  pilB Acinetobacter baumannii D1279779

51.583

96.064

0.496
  pilB Legionella pneumophila strain ERS1305867

47.979

100

0.488
  pilB Vibrio cholerae strain A1552

45.583

100

0.462
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

43.262

100

0.436
  pilF Thermus thermophilus HB27

40.335

96.243

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

95.17

0.385

Multiple sequence alignment