Detailed information NTG1013035

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABQ274_RS00215
• Genome accession: NZ_CP159281
• Coordinates: 33124..33771 (+)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis strain FNZ339
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 28124..38771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABQ274_RS00190 (ABQ274_00190)	-	28253..29431 (+)	1179	WP_012898345.1	SLC13 family permease	-
ABQ274_RS00195 (ABQ274_00195)	-	29604..30167 (+)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
ABQ274_RS00200 (ABQ274_00200)	-	30271..30669 (+)	399	WP_004255236.1	hypothetical protein	-
ABQ274_RS00205 (ABQ274_00205)	-	30774..31706 (+)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
ABQ274_RS00210 (ABQ274_00210)	-	31703..33064 (+)	1362	WP_023164473.1	ABC transporter permease	-
ABQ274_RS00215 (ABQ274_00215)	comEA	33124..33771 (+)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
ABQ274_RS00220 (ABQ274_00220)	comEC	33752..35962 (+)	2211	WP_353727314.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABQ274_RS00225 (ABQ274_00225)	-	36247..37023 (+)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
ABQ274_RS00230 (ABQ274_00230)	-	37208..37423 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
ABQ274_RS00235 (ABQ274_00235)	atpB	37468..38181 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
ABQ274_RS00240 (ABQ274_00240)	atpF	38196..38702 (+)	507	WP_023164471.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=1013035 ABQ274_RS00215 WP_010906131.1 33124..33771(+) (comEA) [Lactococcus lactis strain FNZ339]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=1013035 ABQ274_RS00215 WP_010906131.1 33124..33771(+) (comEA) [Lactococcus lactis strain FNZ339]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386