Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N027_RS23995 Genome accession   NZ_CP159278
Coordinates   5454456..5456561 (-) Length   701 a.a.
NCBI ID   WP_024658016.1    Uniprot ID   A0AAU8M7H8
Organism   Pseudomonas syringae USA007     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5449456..5461561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N027_RS23980 (N027_23980) - 5451081..5452700 (-) 1620 WP_024658018.1 SPOR domain-containing protein -
  N027_RS23985 (N027_23985) aroB 5452711..5453814 (-) 1104 WP_005621200.1 3-dehydroquinate synthase -
  N027_RS23990 (N027_23990) aroK 5453933..5454451 (-) 519 WP_024658017.1 shikimate kinase AroK -
  N027_RS23995 (N027_23995) pilQ 5454456..5456561 (-) 2106 WP_024658016.1 type IV pilus secretin PilQ Machinery gene
  N027_RS24000 (N027_24000) pilP 5456609..5457136 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  N027_RS24005 (N027_24005) pilO 5457133..5457756 (-) 624 WP_003378850.1 type 4a pilus biogenesis protein PilO -
  N027_RS24010 (N027_24010) - 5457753..5458367 (-) 615 WP_024658015.1 PilN domain-containing protein -
  N027_RS24015 (N027_24015) pilM 5458367..5459431 (-) 1065 WP_024658014.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75340.82 Da        Isoelectric Point: 5.1001

>NTDB_id=1013025 N027_RS23995 WP_024658016.1 5454456..5456561(-) (pilQ) [Pseudomonas syringae USA007]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPVPASAKPFIPA
GVKAIRNVDFQRGELGGGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKAEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNG
KWTTYGLDNNGDEAGNTSGNLTSNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=1013025 N027_RS23995 WP_024658016.1 5454456..5456561(-) (pilQ) [Pseudomonas syringae USA007]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATAGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCAGGCGATCGCATCGAGCTCAAGCTTGCCTTTGACGCACCGGTGCCCGCGCCTCGCG
GCTACACCACCGCGCAGCCGGCGCGCATCGCTCTGGACCTGCCGGGCGTGACCAGCCAGCTGGAGACCAAAAGCCGTGAG
CTGGGCACCGGCAATGCACGCAGCGTAGTGGTGGTGCAGGCCCAGGATCGCACGCGAGTGATCATCAACCTGACGACGCT
TGCGCCTTACAGCTCCCGCGTCGACGGCAACAATCTGTTCGTGGTCATTGGCCAGGGTGCCGCCGCAGCGCAGGCTTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTAGCAGTGCCGCCGCCTGTCCCGGCGTCTGCCAAGCCCTTTATACCGGCG
GGCGTCAAAGCCATCCGCAATGTCGATTTTCAGCGCGGTGAGCTGGGTGGAGGCAATGTGGTCATCGACCTGAGTAACCC
GAGCATCGCGCCTGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACCCAGTTGCCCGAGCCTCTGC
GTGTGCGGCTGGACGTCAAGGATTTCGCGACCCCTGTGCAGTTCGTCAGTGCGTCGGCCACGGGCGACAAGGCCAGTATT
CTGATCGAGCCATCGGGTGCCTTCGACTACTCTGCGTTCCAGACCGAAAACAAGCTGACCATCAGCGTGCGTCCGTTGAC
CAACGAAGATCTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTTAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGCCTGCAAAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAACGTAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCAGCGGATGAAATTGCCGCCCGCGAACGCCAGGAGCTGGAATCGCTCAAGCAGATTG
CCGAACTGGCACCGCTGCGCCGCGAGCTGCTGCAGGTCAATTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCCAGTCG
GTGACCAGTGCCGAGTCCAAGGCTGAAGAGCGTGGCTCGATCACCGTGGACGACCGCACCAACAACATCATTGCTTACCA
GACCCAGGACCGTCTCGACGAACTGCGTCGCATCGTTTCGCAACTGGACATCCCGGTGCGCCAAGTGATGATCGAAGCAC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTGGGCGTACGCTGGGGCGGTTCGACCAACACCAGCGGCAACGGG
AAATGGACGACGTACGGGCTGGATAACAATGGCGATGAGGCCGGTAACACGAGCGGCAATCTGACTTCCAACGTTCCGTT
CGTAGACTTGGGGGCCGCCGGGGCGACGTCCGGCATCGGCCTGGGCTTTGTGACCAATAACACCTTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGCAACGGCGAAATCGTCTCCCAGCCCAAAGTGGTCACGTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACAGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACCACCGTAAGCTTCAAGGAGGCTTCGCT
GTCGCTTGAAGTCACGCCGCAGATTACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCGCCGATCAAGAAAAACGAGGTTAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTGATCGGCGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.476

100

0.748
  comQ Acinetobacter baylyi ADP1

41.774

99.715

0.417
  pilQ Acinetobacter baumannii strain A118

38.753

100

0.408
  pilQ Acinetobacter baumannii D1279779

38.753

100

0.408
  pilQ Legionella pneumophila strain Lp02

38.986

98.431

0.384
  pilQ Legionella pneumophila strain ERS1305867

38.653

97.432

0.377

Multiple sequence alignment