Detailed information NTG1011934

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABUH86_RS12415
• Genome accession: NZ_CP158669
• Coordinates: 2554805..2555419 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain XY40-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2549805..2560419

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABUH86_RS12385 (ABUH86_12385)	rpsT	2550108..2550374 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ABUH86_RS12390 (ABUH86_12390)	holA	2550391..2551432 (-)	1042	Protein_2393	DNA polymerase III subunit delta	-
ABUH86_RS12395 (ABUH86_12395)	-	2551472..2551623 (-)	152	Protein_2394	hypothetical protein	-
ABUH86_RS12400 (ABUH86_12400)	-	2551664..2551798 (+)	135	WP_003152870.1	YqzM family protein	-
ABUH86_RS12405 (ABUH86_12405)	comEC	2551817..2554168 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABUH86_RS12410 (ABUH86_12410)	-	2554169..2554738 (-)	570	WP_094031848.1	ComE operon protein 2	-
ABUH86_RS12415 (ABUH86_12415)	comEA	2554805..2555419 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
ABUH86_RS12420 (ABUH86_12420)	comER	2555478..2556299 (+)	822	WP_012118027.1	late competence protein ComER	-
ABUH86_RS12425 (ABUH86_12425)	-	2556368..2557105 (-)	738	WP_076424998.1	class I SAM-dependent methyltransferase	-
ABUH86_RS12430 (ABUH86_12430)	rsfS	2557102..2557458 (-)	357	WP_007408260.1	ribosome silencing factor	-
ABUH86_RS12435 (ABUH86_12435)	yqeK	2557476..2558036 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ABUH86_RS12440 (ABUH86_12440)	-	2558026..2558595 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ABUH86_RS12445 (ABUH86_12445)	yhbY	2558606..2558896 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ABUH86_RS12450 (ABUH86_12450)	aroE	2558890..2559732 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=1011934 ABUH86_RS12415 WP_043021637.1 2554805..2555419(-) (comEA) [Bacillus velezensis strain XY40-1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1011934 ABUH86_RS12415 WP_043021637.1 2554805..2555419(-) (comEA) [Bacillus velezensis strain XY40-1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363